Ecology of Viruses Isolated from Sand Flies in Italy and Characterization of a New Phlebovirus (Arbia Virus)

Verani, P; Ciufolini, Maria Grazia; Caciolli, S; Renzi, Alessia; Nicoletti, Loredana; Sabatinelli, Guido; Bartolozzi, D; Volpi, G; Amaducci, L.; Cóluzzi, M.

doi:10.4269/ajtmh.1988.38.433

Cited by 109 publications

(95 citation statements)

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“…Its genetic distance with known TOSV was 10.2% (Figure 2A), thus confirming the persistence of TOSV in populations of P. perfiliewi in central Italy. The overall minimum field infection rate was higher (1.0) than that previously recorded in Italy, 4,11 indicating possible annual variations in infection rates.…”

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confidence: 46%

“…Furthermore, four isolates that were negative by nested PCR amplification for members of the genus Phlebovirus showed a cytopathic effect in Vero cell cultures and their identification is in progress. Previous studies of viral ecology showed the simultaneous presence of TOSV and ARBV in the same population of a sandfly species, 4 as well as mechanisms for potential virus maintenance and amplification by transovarial 11 and venereal transmission. 12 These ecologic niche characteristics, associated with possible co-infections in the same vector, could result in the emergence of reassortants of viruses with different degrees of pathogenecity.…”

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confidence: 99%

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Viral Isolates of a Novel Putative Phlebovirus in the Marche Region of Italy

Remoli

Fortuna

Marchi

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Thirty pools from 900 (540 females and 360 males) Phlebotomus perfiliewi sandflies collected during the summer of 2012 in the Fermo area (Marche Region, central Italy) were tested for the presence of Phleboviruses. A nested polymerase chain reaction was performed using degenerated primers amplifying a fragment of the polymerase gene (large segment) and a fragment of the nucleoprotein gene (small segment) of the genus Phlebovirus. One pool was positive for Toscana virus, as expected from results of studies in the area, and six pools were positive for a putative novel Phlebovirus. Virus isolation in Vero cells was performed. Minimum field infection rates/1,000 insects processed for the novel and Toscana viruses were 6.7 and 1.0, respectively. Phylogenetic analysis of the novel Phlebovirus, tentatively named Fermo virus, placed it in the Sandfly Fever Naples virus serocomplex.

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confidence: 46%

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confidence: 99%

Viral Isolates of a Novel Putative Phlebovirus in the Marche Region of Italy

Remoli

Fortuna

Marchi

et al. 2014

The American Society of Tropical Medicine and Hygiene

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“…According to ICTV Salehabad virus consists of two viruses: Salehabad virus isolated in 1959 from sand flies in Iran (40), and Arbia virus, isolated in 1988 from sand flies in Italy (41).…”

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confidence: 99%

Isolation, Genetic Characterization, and Seroprevalence of Adana Virus, a Novel Phlebovirus Belonging to the Salehabad Virus Complex, in Turkey

Alkan

Alwassouf

Piorkowski

et al. 2015

J Virol

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A new phlebovirus, Adana virus, was isolated from a pool of Phlebotomus spp. (Diptera; Psychodidae) in the province of Adana, in the Mediterranean region of Turkey. Genetic analysis based on complete coding of genomic sequences indicated that Adana virus belongs to the Salehabad virus species of the genus Phlebovirus in the family Bunyaviridae. Adana virus is the third virus of the Salehabad virus species for which the complete sequence has been determined. To understand the epidemiology of Adana virus, a seroprevalence study using microneutralization assay was performed to detect the presence of specific antibodies in human and domestic animal sera collected in Adana as well as Mersin province, located 147 km west of Adana. The results demonstrate that the virus is present in both provinces. High seroprevalence rates in goats, sheep, and dogs support intensive exposure to Adana virus in the region, which has not been previously reported for any virus included in the Salehabad serocomplex; however, low seroprevalence rates in humans suggest that Adana virus is not likely to constitute an important public health problem in exposed human populations, but this deserves further studies. IMPORTANCE Until recently, in the genus

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“…Toscana virus (TOSV) is an arthropod-borne virus (Phlebovirus genus, Bunyaviridae family) transmitted by sand flies of Phlebotomus species (Phlebotomus perniciosus and Phlebotomus perfiliewi) residing in humid areas around the Mediterranean (11,14). TOSV has been associated with acute meningitis and encephalitis occurring during the summer in individuals living in central Italy (1,7,12), Spain (3,6), and France (5), as well as in travelers returning from Mediterranean countries (2,4,10).…”

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confidence: 99%