Economic analysis of automated micropropagation

Chu, Irwin Y. E.

doi:10.1007/978-94-015-8461-6_2

Cited by 28 publications

(6 citation statements)

References 2 publications

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“…Automation is a practical strategy since in vitro propagation necessitates a lot of repetitive effort and is time-consuming, especially if the output is kept to a minimum during the acclimation period due to plantlet mortality. Reduced contamination risk and decreased labor cost are two additional advantages of automating the acclimatization process [51][52][53][54]. Modern precision agriculture practices, including glasshouse technology, are largely automated, and information technology paired with IoT (internet of things) technology is well-developed and efficient.…”

Section: Introductionmentioning

confidence: 99%

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

2022

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One of the technological advancements in agricultural production is the tissue culture propagation technique, commonly used for mass multiplication and disease-free plants. The necessity for date palm tissue culture emerged from the inability of traditional propagation methods’ offshoots to meet the immediate demands for significant amounts of planting material for commercial cultivars. Tissue culture plantlets are produced in a protected aseptic in vitro environment where all growth variables are strictly controlled. The challenges occur when these plantlets are transferred to an ex vitro climate for acclimatization. Traditional glasshouses are frequently used; however, this has substantial mortality consequences. In the present study, a novel IoT-based automated ex vitro acclimatization system (E-VAS) was designed and evaluated for the acclimatization of date palm plantlets (cv. Khalas) to enhance their morpho-physiological attributes and reduce the mortality rate and the contamination risk through minimal human contact. The experimental findings showed that the morpho-physiological parameters of 6- and 12-month-old plants were higher when acclimatized in the prototype E-VAS compared to the traditional glasshouse acclimatization system (TGAS). The maximum plant mortality percentage occurred within the first month of the transfer from the in vitro to ex vitro environment in both systems, which gradually declined up to six months; after that, no significant plant mortality was observed. About 6% mortality was recorded in E-VAS, whereas 18% in TGAS within the first month of acclimatization. After six months of study, an overall 14% mortality was recorded in E-VAS compared to 41% in TGAS. The proposed automated system has a significant potential to address the growing demand for the rapid multiplication of tissue culture-produced planting materials since the plant survival rate and phenotype quality were much higher in E-VAS than in the conventional manual system that the present industry follows for commercial production.

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Section: Introductionmentioning

confidence: 99%

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

2022

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“…Also, encapsulation of propagules that were produced in vitro could reduce the cost of micropropagation of plantlets for commercialization and final delivery (Chu 1995, Nieves et al 2003. This technology may be of value in breeding programs and allows the propagation of many elite genotypes in a short time (Nieves et al 1998).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

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“…The high cost of tissue culture plants is a result of labour-intensive operations, especially for propagation (stages 1 and 2) which need to be done repeatedly to obtain large numbers of reproductions. Chu (1995) analysed a private company and noted that labour costs accounted for 50% of the total cost of a tissue culture plant. Zandvoort and Holdgate (1991) stated that labour costs as a portion of total production cost ranged from 13 to 41% in India, 60 to 85% in US and 70% in Europe.…”

Section: Introductionmentioning

confidence: 99%