Economic production of Bacillus subtilis SPB1 biosurfactant using local agro‐industrial wastes and its application in enhancing solubility of diesel

Abstract

BACKGROUND: The present work aimed to optimize a new economic medium for lipopeptide biosurfactant production by Bacillus subtilis SPB1 for application in the environmental field as an enhancer of diesel solubility. Statistical experimental designs and response surface methodology were employed to optimize the medium components. RESULTS: A central composite design was applied to increase the production yield and predict the optimal values of the selected factors. An optimal medium, for biosurfactant production of about 4.5 g L−1, was found to be composed of sesame peel flour (33 g L−1) and diluted tuna fish cooking residue (40%) with an inoculum size of 0.22. Increased inoculum size (final OD600) significantly improved the production yield. The emulsifier produced was demonstrated to be an alternative to chemically synthesized surfactants since it shows high solubilization efficiency towards diesel oil in comparison with SDS and Tween 80. CONCLUSION: Optimization studies led to a strong improvement in production yield. The emulsifier produced, owing its high solubilization capacity and its large tolerance to acidic and alkaline pH values and salinity, shows great potential for use in bioremediation processes to enhance the solubility of hydrophobic compounds. © 2012 Society of Chemical Industry

Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation

“…This result is in very close agreement with the findings of Huszcza and Burczyk [29], who reported that the biosurfactant activity produced by B. coagulans was enhanced with the addition of salts. In addition, the maximum activity of the biosurfactant produced by B. subtilis was achieved with the addition of 15% NaCl [30]. The current results also indicated that B. salmalaya can grow under conditions of high salinity.…”

Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

“…This suggests the competence of B. subtilis biosurfactant in promoting methyl orange treatment by A. veronii GRI. SPB1-derived , +0.15 % biosurfactant biosurfactant has been previously described as enhancer of diesel solubility and mobility (Mnif et al 2012b(Mnif et al , 2013. In another study, in situ SPB1 biosurfactant production (Mnif et al 2014) and ex situ SPB1 biosurfactant addition were reported to enhance hydrocarbon assimilation .…”

Lipopeptide surfactants: Production, recovery and pore forming capacity

“…A variety of cheap raw materials, including plant-derived oils, oil wastes, starchy substances, lactic whey and distillery wastes have been reported to support biosurfactant production. Previous studies reported the use of rice bran [54], soy bean flour [55,56], soybean curd residue [57], potato process effluents [55], potato peels [58,59], sesame peel flour [60], orange peels [61], millet flour [62] tuna fish flour [59], molasses and whey [63], tuna fish cooking residue [60] and soybean sauce [64] for lipopeptide production. Also, numerous studies reported the production of lipopeptide using cassava wastewater as substrate [65][66][67].…”