Economical novel formulation and evaluation of herbal oils for mosquito and house fly repellent activities

Abstract

Aromatic pla nts conta in compou nds that they use in preventing attack from phytophagous insects with the multiple mechanisms like repellents, feeding deterrents, toxins, and growth regulators etc. Looking at that the present study was carried out with the aim of mosquito and house fly repellent activities with the novel herbal oil formulations. The oils of patchouli, eucalyptus, rosemary, citronella and neem leaves were extracted by hydro distillation method using Clevenger apparatus and various formulations were prepared, viz., tincture, candle and crystal cake. Tincture was evaluated by sprayed in known mosquito larvae and observed for death rate using acetone as control; candle was evaluated on flammability, burning time as well as mosquito and insect repellency test. Furthermore crystal cake formulation was evaluated on appearance, volatility time, stability of fragrance, mosquito and insect repellency test. All the formulations showed remarkable significant dual activities against mosquito and insect population. Based on these preliminary actions, all these formulations were tested in Varthur locality (30 houses and 20 chicken shops) for one month where mosquito and insect populations were more and resulted significant elimination of both the populations. This resu lt may be due to the presence of the active constituents like volatile alcohol, ketone and other constituents in the oils. The result revealed the formulated tincture spray and candle were more effective than crystal cake in relation to killing mosquitoes, insects, stability of fragrance, etc.

Bio-Insecticidal Nanoemulsions of Essential Oil and Lipid-Soluble Fractions of Pogostemon cablin

“…A preliminary investigation on NEs of P. cablin oil (18%) with polyoxyethylene suggested excellent formicidal action on Atta opaciceps and Atta sexdens (Rocha et al, 2018). Furthermore, P. cablin oil-based tincture, candle, and crystal cake have been found effective as mosquito repellent (Das et al, 2016). Recently, patchouli oil has been nano-encapsulated on chitosan and evaluated for enhanced shelf life of maize seeds (Roshan et al, 2022).…”

Metabolite Profiling and Quantitation of Cucurbitacins in Cucurbitaceae Plants by Liquid Chromatography coupled to Tandem Mass Spectrometry

“…We present herein a comprehensive study focusing on the generation of metabolic profile of various plants of the Cucurbitaceae family, along with quantitation of four biologically active cucurbitacins in ten species belonging to six different genera of the Cucurbitaceae family, along with Cissampelos pareira belonging to the Menispermaceae family. C. pareira is also reported to possess antioxidant 19 , anti-inflammatory 20 , antiviral 21 , antidiabetic 22 , anticancer 23,24 and other activities 25 . There are studies in literature that report of the profiling of Cucurbitaceae family plants, such as profiling of phenolics and other polar components in watermelon 26 and zucchini 27 through LC-MS/MS.…”