Ecotoxicological analysis during the removal of carbofuran in fungal bioaugmented matrices

Ruíz-Hidalgo, Karla; Masís-Mora, Mario; Barbieri, Edison; Carazo-Rojas, Elizabeth; Rodríguez-Rodríguez, Carlos E.

doi:10.1016/j.chemosphere.2015.09.056

Cited by 34 publications

(12 citation statements)

References 34 publications

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“…To determine whether the fungal removal process is environmentally relevant, an ecotoxicological assay was employed to confirm detoxification, as some transformation products from pesticide degradation might present higher toxicity than the original compound (Ruíz-Hidalgo et al, 2016). An acute toxicity test was performed with the freshwater microcrustacean D. magna using untreated samples (initial work pesticide solutions in liquid culture media) and samples collected after 14 days for each treatment (abiotic and heat-killed controls, and viable fungus).…”

Section: Ecotoxicity Assay: Immobilization Test In Daphnia Magnamentioning

confidence: 99%

Tolerance and Biological Removal of Fungicides by Trichoderma Species Isolated From the Endosphere of Wild Rubiaceae Plants

Escudero-Leyva

Alfaro-Vargas²,

Muñoz-Arrieta³

et al. 2022

Front. Agron.

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The transition from conventional to organic agriculture is often challenged by the adaptation of biological control agents to environments heavily exposed to agrochemical pollutants. We studied Trichoderma species isolated from living leaf tissues of wild Rubiacaeae (coffee family) plants to determine their fungicide tolerance and potential for bioremoval. First, we assessed the in vitro tolerance to fungicides of four Trichoderma isolates (Trichoderma rifaii T1, T. aff. crassum T2, T. aff. atroviride T3, and T. aff. strigosellum T4) by placing mycelial plugs onto solid media supplemented with seven different systemic and non-systemic fungicides. After a week, most of the fungicides did not significantly inhibit the growth of the isolates, except in the case of cyproconazole, where the only isolate able to grow was T1; however, the colony morphology was affected by the presence of fungicides. Second, biological removal potential was established for selected isolates. For this experiment, the isolates T1, T2, and T4 were independently inoculated into liquid media with the fungicides azoxystrobin, chlorothalonil, cyproconazole, and trifloxystrobin. After 14 days of incubation, a removal of up to 89% was achieved for chlorothalonil, 46.4% for cyproconazole, and 33.1% for trifloxystrobin using viable biomass. In the case of azoxystrobin, the highest removal (82.2%) occurred by adsorption to fungal biomass. Ecotoxicological tests in Daphnia magna revealed that T1 has the highest removal potential, achieving significant elimination of every fungicide, while simultaneously detoxifying the aqueous matrix (except in the case of cyproconazole). Isolate T4 also exhibited an intermediate efficiency, while isolate T2 was unable to detoxify the matrix in most cases. The removal and detoxification of cyproconazole failed with all the isolates. These findings suggest that endosphere of wild plants could be an attractive guild to find new Trichoderma species with promising bioremediation capabilities. In addition, the results demonstrate that attention should be placed when combining certain types of agrochemicals with antagonistic fungi in Integrated Pest and Disease Management strategies or when transitioning to organic agriculture.

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Section: Ecotoxicity Assay: Immobilization Test In Daphnia Magnamentioning

confidence: 99%

Tolerance and Biological Removal of Fungicides by Trichoderma Species Isolated From the Endosphere of Wild Rubiaceae Plants

Escudero-Leyva

Alfaro-Vargas²,

Muñoz-Arrieta³

et al. 2022

Front. Agron.

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“…Ruiz-Hidalgo et al (2014) used rice husk inoculated with Trametes versicolor to degrade a 55% of carbofuran in 34 days using a biomixture. In addition, Ruíz-Hidalgo et al (2016) also evaluated the optimization of a bioaugmented biomixture for the degradation of carbofuran using T. versicolor. They found the use of rice husk as bioaugmentation agent supports the cost-effectiveness of the pesticide degradation in the system.…”

Section: Discussionmentioning

confidence: 99%

Immobilization of the white-rot fungus Anthracophyllum discolor to degrade the herbicide atrazine

et al. 2016

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Herbicides cause environmental concerns because they are toxic and accumulate in the environment, food products and water supplies. There is a need to develop safe, efficient and economical methods to remove them from the environment, often by biodegradation. Atrazine is such herbicide. White-rot fungi have the ability to degrade herbicides of potential utility. This study formulated a novel pelletized support to immobilize the white-rot fungus Anthracophyllum discolor to improve its capability to degrade the atrazine using a biopurification system (BS). Different proportions of sawdust, starch, corn meal and flaxseed were used to generate three pelletized supports (F1, F2 and F3). In addition, immobilization with coated and uncoated pelletized supports (CPS and UPS, respectively) was assessed. UPS-F1 was determined as the most effective system as it provided high level of manganese peroxidase activity and fungal viability. The half-life (t1/2) of atrazine decreased from 14 to 6 days for the control and inoculated samples respectively. Inoculation with immobilized A. discolor produced an increase in the fungal taxa assessed by DGGE and on phenoloxidase activity determined. The treatment improves atrazine degradation and reduces migration to surface and groundwater.

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“…Despite the fact that pesticides guarantee an increase in agricultural productivity, by protecting crops from their "natural enemies", their inadequate management can compromise soils, water sources and, consequently, non-target organisms (Gunningham and Sinclair 2005;Campos-Garcia et al 2016). In this sense, the constant use of these substances causes great concern, because once present in the environment, they affect all the biota that is exposed to them in different ways (Barbieri et al 2013;Santiago-Moreira 2013;Ruíz-Hidalgo et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Effect of exposure to carbofuran on the behavior and metabolism of the Atlantic Forest lambari Deuterodon iguape (Eigenmann 1907), a native species from Brazil

Mendes

Henriques

Barbieri

2021

Preprint

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One of the major causes of aquatic biodiversity loss is the contamination of the environment by pesticides. Although there is a considerable amount of studies on the subject, there are still few that deal with the effects of carbofuran on native species in Brazil. Although carbofuran is widely used in Brazil, its action on native organisms, such as the Atlantic Forest lambari Deuterodon iguape , has not yet been studied. This work aimed to evaluate the effects of exposure to carbofuran on the fish D. iguape , considering the behavior and specific oxygen consumption and specific ammonia excretion as end points. Opercular movements, dorsal fin movements and swimming speed were analyzed as behavioral parameters. To assess specific oxygen consumption and specific ammonia excretion, fish were subjected to concentrations of 0.0, 0.05, 0.1, 0.25, and 0.5 mg/L, for 24 hours. For behavior analysis, fish remained exposed to carbofuran at concentrations: 0.0, 0.01, 0.05, 0.1 and 0.5 mg/L, in periods of 0, 2, 24 and 48 hours. The behavior was studied through filming, analyzed with the free software, Tracker 4.92 (Open Source Physics). The results showed that there was a decrease in opercular movements (-8%±2.65), in dorsal fin movements (-18.6%±2.97), as well as in swimming speed (-66.4%±1.83). There was an increase in oxygen consumption of 58.4% and ammonia excretion by 90.6% in fish exposed to the highest concentration of carbofuran. Thus, it is concluded that carbofuran altered D. iguape 's behavior, oxygen consumption and ammonia excretion. The species was sensitive to carbofuran concentrations and can be used as a bioindicator.

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Ecotoxicological analysis during the removal of carbofuran in fungal bioaugmented matrices

Cited by 34 publications

References 34 publications

Tolerance and Biological Removal of Fungicides by Trichoderma Species Isolated From the Endosphere of Wild Rubiaceae Plants

Tolerance and Biological Removal of Fungicides by Trichoderma Species Isolated From the Endosphere of Wild Rubiaceae Plants

Immobilization of the white-rot fungus Anthracophyllum discolor to degrade the herbicide atrazine

Effect of exposure to carbofuran on the behavior and metabolism of the Atlantic Forest lambari Deuterodon iguape (Eigenmann 1907), a native species from Brazil

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