Immobilization of the white-rot fungus Anthracophyllum discolor to degrade the herbicide atrazine

Abstract

Herbicides cause environmental concerns because they are toxic and accumulate in the environment, food products and water supplies. There is a need to develop safe, efficient and economical methods to remove them from the environment, often by biodegradation. Atrazine is such herbicide. White-rot fungi have the ability to degrade herbicides of potential utility. This study formulated a novel pelletized support to immobilize the white-rot fungus Anthracophyllum discolor to improve its capability to degrade the atrazine using a biopurification system (BS). Different proportions of sawdust, starch, corn meal and flaxseed were used to generate three pelletized supports (F1, F2 and F3). In addition, immobilization with coated and uncoated pelletized supports (CPS and UPS, respectively) was assessed. UPS-F1 was determined as the most effective system as it provided high level of manganese peroxidase activity and fungal viability. The half-life (t1/2) of atrazine decreased from 14 to 6 days for the control and inoculated samples respectively. Inoculation with immobilized A. discolor produced an increase in the fungal taxa assessed by DGGE and on phenoloxidase activity determined. The treatment improves atrazine degradation and reduces migration to surface and groundwater.

Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates

“…LEJ701 and LEJ702 (Ellegaard‐Jensen et al, 2013). Elgueta et al (2016) formulated a novel pelletized support to immobilize Anthracophyllum discolor , a white rot fungus, for improving its ability to degrade the atrazine, a herbicide, by a bio‐purification system and for generating three pelletized supports (F1, F2, and F3), different proportions of starch, sawdust, flaxseed, and corn meal were used. The most effective system was F1 as it provided great manganese peroxidase activity and fungal viability (Elgueta et al, 2016).…”

“…intermedia DP8-1(Wang et al, 2017), Mortierella sp.LEJ701 and LEJ702 (Ellegaard-Jensen et al, 2013) Elgueta et al (2016). formulated a novel pelletized support to immobilize Anthracophyllum discolor, a white rot fungus, for improving its ability to degrade the atrazine, a herbicide, by a bio-purification system and for generating three pelletized supports (F1, F2, and F3), different proportions of starch, sawdust, flaxseed, and corn meal were used.…”

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

“…Laccase was extracted according to Elgueta et al [54], and the activity was measured by mixing 0.5 mL of the extracted sample, 0.25 mL of 100 mM glycine (pH 3.0), and 0.25 mL of 4 mM ABTS, and incubating at 25 • C for 5 min before the measurement. Then, 1 mL of the sample was used for Laccase determination by spectrophotometry at 405 nm in a BK-UV1800 spectrophotometer (Biobase).…”

Microbial Degradation of Pesticide Residues and an Emphasis on the Degradation of Cypermethrin and 3-phenoxy Benzoic Acid: A Review

“…Jayabarath et al [ 53 ] selected 319 actinomycetes from saline soils of Sangli District (Maharashtra) for carbofuran tolerance test, while only the seven strains of Streptomyces alanosinicus, Streptoverticillium album, Nocardia farcinia, Streptomyces atratus, Nocardia vaccini, Nocardia amarae, and Micromonospora chalcea can grow and degrade pesticides very well. Elgueta et al [ 54 ] used white-rot fungi to study the degradation of atrazine and found that the half-life of atrazine decreased to six days. Besides, Kabra et al [ 55 ] studied the ability of green microalga Chlamydomonas mexicana to degrade atrazine and found that microalgae could effectively degrade atrazine by accumulating atrazine in cells and then degrading it, reaching a degradation rate of 14–36%.…”