Ecotoxicological effects of erythromycin on a multispecies biofilm model, revealed by metagenomic and metabolomic approaches

Yang, Pu; Pan, Jie; Yao, Yuan; Ngan, Wing Yui; Yang, Yang; Li, Meng; Habimana, Olivier

doi:10.1016/j.envpol.2021.116737

Cited by 20 publications

(12 citation statements)

References 47 publications

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“…6). River biofilms exposed to erythromycin and Ag nanoparticles had also showed a change in their meta-metabolome, while prokaryotic community diversity appeared unaffected after 6 and 12 weeks of exposure, respectively (Pu et al, 2021; Yang et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

Colas,

Marie,

Morin

et al. 2023

Preprint

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Untargeted metabolomics is a non-a priorianalysis of biomolecules that characterizes the metabolome variations induced by short- and long-term exposures to stressors. Even if the metabolite annotation remains lacunar due to database gaps, the global metabolomic fingerprint allows for trend analyses of dose-response curves for hundreds of cellular metabolites. The combination of untargeted metabolomic features and benchmark-dose (BMD) calculations then makes it possible to determine concentration range inducing defense responses (CRIDeR) and concentration range inducing damage responses (CRIDaR). To develop this approach in a context of time-dependent microbial community changes, mature river biofilms were exposed for 1 month to four cobalt (Co) concentrations (background concentration, 1 x 10-7, 5 x 10-7and 1 x 10-6M) in an open system of artificial streams. The meta-metabolomic response of biofilms was compared against a multitude of biological parameters (including bioaccumulation, biomass, chlorophyllacontent, composition and structure of prokaryotic and eukaryotic communities) monitored at set exposure times (from 1 hour to 28 days). Cobalt exposure induced extremely rapid responses of the meta-metabolome, with time range inducing defense responses (TRIDeR) of around ten seconds, and time range inducing damage responses (TRIDaR) of several hours. Even in biofilms whose structure had been altered by Co bioaccumulation (reduced biomass, chlorophyllacontents and changes in the composition and diversity of prokaryotic and eukaryotic communities), CRIDeRs with similar initiation thresholds (1.41 ± 0.77 x 10-10M Co2+added in the exposure medium) were set up at the meta-metabolome level at every time point. In contrast, the CRIDaR initiation thresholds increased by 10 times in long-term Co exposed biofilms. The present study demonstrates that defense and damage responses of biofilm meta-metabolome exposed to Co are rapidly and sustainably impacted, even within tolerant and resistant microbial communities.Graphical abstractHighlightsProkaryotic community structures were impacted after 1 h of exposure to CoBiofilm meta-metabolome was impacted after 36 s of exposure to CoBiofilm meta-metabolome response was faster than changes in biofilm communitiesShort- and long-term exposed biofilms have similar CRIDeR initiation thresholdsLong-term exposed biofilms have higher CRIDaR initiation thresholds

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Section: Discussionmentioning

confidence: 99%

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

Colas,

Marie,

Morin

et al. 2023

Preprint

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“…This may indicate that EPS can act as a protective barrier for biofilm and increase its resistance to antibiotics, as noted above (Robson et al, 2020). Pu et al (2021) studied the effects of erythromycin (6 μg/L) on freshwater biofilms. The biofilm used in this study was cultured in reactors, in aquarium water, allowing the lab to replicate a freshwater environment.…”

Section: Antibioticsmentioning

confidence: 92%

Do pharmaceuticals affect microbial communities in aquatic environments? A review

Świacka

Maculewicz

Kowalska

et al. 2023

Front. Environ. Sci.

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Pharmaceuticals have been identified as a significant threat to the environment. Their constant flow into aquatic ecosystems means that organisms are chronically exposed. To date, there has been a large number of scientific papers assessing the impact of pharmaceuticals on individual organisms from different taxonomic groups. However, the effects of drugs on the environment can be much broader than what can be determined in toxicity tests on individual organisms. These compounds can disrupt entire communities. In this context, special attention should be paid to microbial communities, which regulate many essential processes underpinning aquatic food webs and ecosystem services. This paper reviews current developments related to the effects of pharmaceuticals on microorganisms with a particular focus on whole-community investigations, in both fresh and salt water. We also summarize the opportunities associated with both in situ and laboratory studies, and highlight important knowledge gaps.

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“…The intensity of each peak was recorded, and a three-dimensional matrix containing arbitrarily assigned peak indices (retention time– m / z pairs), sample names (observations), and ion intensity information (variables) was generated. Data preprocessing method are described in the literature ( Pu et al., 2021 ). Briefly, the primary identification of metabolites was carried out using the Human Metabolome Database (HMDB) and Kyoto Encyclopedia of Genes and Genomes database (KEGG).…”

Section: Methodsmentioning

confidence: 99%

Metabonomic profiling of clubroot-susceptible and clubroot-resistant radish and the assessment of disease-resistant metabolites

Huang

et al. 2022

Front. Plant Sci.

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Plasmodiophora brassicae causes a serious threat to cruciferous plants including radish (Raphanus sativus L.). Knowledge on the pathogenic regularity and molecular mechanism of P. brassicae and radish is limited, especially on the metabolism level. In the present study, clubroot-susceptible and clubroot-resistant cultivars were inoculated with P. brassicae Race 4, root hairs initial infection of resting spores (107 CFU/mL) at 24 h post-inoculation and root galls symptom arising at cortex splitting stage were identified on both cultivars. Root samples of cortex splitting stage of two cultivars were collected and used for untargeted metabonomic analysis. We demonstrated changes in metabolite regulation and pathways during the cortex splitting stage of diseased roots between clubroot-susceptible and clubroot-resistant cultivars using untargeted metabonomic analysis. We identified a larger number of differentially regulated metabolites and heavier metabolite profile changes in the susceptible cultivar than in the resistant counterpart. The metabolites that were differentially regulated in both cultivars were mostly lipids and lipid-like molecules. Significantly regulated metabolites and pathways according to the P value and variable important in projection score were identified. Moreover, four compounds, including ethyl α-D-thioglucopyranoside, imipenem, ginsenoside Rg1, and 6-gingerol, were selected, and their anti-P. brassicae ability and effects on seedling growth were verified on the susceptible cultivar. Except for ethyl α-D-thioglucopyranoside, the remaining could inhibit clubroot development of varing degree. The use of 5 mg/L ginsenoside Rg1 + 5 mg/L 6-gingerol resulted in the lowest disease incidence and disease index among all treatments and enhanced seedling growth. The regulation of pathways or metabolites of carbapenem and ginsenoside was further explored. The results provide a preliminary understanding of the interaction between radish and P. brassicae at the metabolism level, as well as the development of measures for preventing clubroot.

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Ecotoxicological effects of erythromycin on a multispecies biofilm model, revealed by metagenomic and metabolomic approaches

Cited by 20 publications

References 47 publications

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

Do pharmaceuticals affect microbial communities in aquatic environments? A review

Metabonomic profiling of clubroot-susceptible and clubroot-resistant radish and the assessment of disease-resistant metabolites

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