Ecotoxicology of Glyphosate-Based Herbicides on Aquatic Environment

Gonçalves, Bruno Bastos; Giaquinto, Percília Cardoso; Silva, Douglas dos Santos; Neto, Carlos de Melo e Silva; Lima, Amanda Alves de; Darosci, Adriano Antonio; Portinho, Jorge Laço; Carvalho, Wanessa Fernandes; Rocha, Thiago Lopes

doi:10.5772/intechopen.85157

Cited by 15 publications

(13 citation statements)

References 89 publications

(114 reference statements)

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“…The compound might also be genotoxic to cells, a fact substantiated by histopathological tests in fish hepatocytes where vacuolization and cytoplasm degradation were observed (Samsel and Seneffl, 2015 ). A biochemical analysis of the liver, heart, and kidneys conducted on freshwater fish Cyprinus Carpio L. documented an increase in the activity of alkaline phosphatase and the activity of glutamine-oxaloacetate transaminase (Gonçalves et al, 2019 ). Furthermore, GBH reduced the activity of antioxidant enzymes (such as catalase, glutathione peroxidase, and superoxide dismutase) in fish species like rainbow trout (Hidalgo et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, to mitigate side effects, governments have established limits on the usage and concentration of glyphosate-based pesticides in water reservoirs. For instance, the US permits 700 μg/l in tank water, Canada allows 280 μg/l in drinking water, while European regulations are more strict, permitting only 0.1 μg/l, thereby potentially offering more restrictive in terms of protecting aquatic biodiversity (Gonçalves et al, 2019 ). A study showed that concentrations of glyphosate below 0.1 μg/l present a low risk, while those above 1 μg/l – a level below the legal limit in some countries – pose a significant risk to aquatic organisms (Rehman et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide

KWIATKOWSKA

2023

bta

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The aim of this study was to investigate the effect of Roundup ® herbicide on the maturation of Prussian carp oocytes under laboratory conditions. The Prussian carp is currently one of the most common fish species in Polish freshwater ichthyofauna. For the investigation, oocytes from five sexually mature female Prussian carp were used, segmented into three groups, and incubated for 24 h in Cortland’s saline, treated with varied concentrations of the herbicide Roundup ® (0 ng – control, R1 – 10 ng/ml, and R2 – 100 ng/ml). Subsequent to this period, assays were performed using the prepared plates to determine the level of 17α,20β-dihydroxyprogesterone (17α,20β-P) utilizing the standard ELISA technique. In determining the 17α,20β-P via ELISA, the medium was extracted from each tested oocyte group. Oocyte maturity was assessed through preservation in serra fluid, and, to categorize the maturity stage of the oocytes utilizing a four-point scale – contingent upon the nucleus’s position – the formerly preserved oocytes were dehydrated and subsequently analyzed. A contrast was noted in the percentage of oocytes at varied stages between the control group and the experimental groups. Specifically, a higher concentration of Roundup ® (100 ng/ml) accelerated to expedite the initial migration of the nucleus in oocytes. In conclusion, the obtained results show the adverse effect of Roundup ® on hormonal regulation and maturation in Prussian carp oocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide

KWIATKOWSKA

2023

bta

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“…Furthermore, it emerges that the most frequently tested heavy metals are copper (Cu), cadmium (Cd), chromium (Cr), and zinc (Zn), while the plant species that are most widely used to test their effects are listed in Supplementary Table 1. The most commonly detected responses by these species in the presence of heavy metals are: reduction in the growth rate, increase in the rate of necrosis and chlorosis, oxidative stress and photosynthesis inhibition (Monferran et al 2009;Razinger et al 2010;Upadhyay et al 2011;Corcoll et al 2012), increase in the content of malondialdehyde (e.g., Das et al 2016;Decou et al 2018), and antioxidant responses (Gonçalves et al 2019;Li et al 2018).…”

Section: Heavy Metalmentioning

confidence: 99%

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Ceschin

Bellini

Scalici

2020

Environ Sci Pollut Res

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This paper reviews the current state-of-the-art, limitations, critical issues, and new directions in freshwater plant ecotoxicology. We selected peer-reviewed studies using relevant databases and for each (1) publication year, (2) test plant species, (3) reference plant group (microalgae, macroalgae, bryophytes, pteridophytes, flowering plants), (4) toxicant tested (heavy metal, pharmaceutical product, hydrocarbon, pesticide, surfactant, plastic), (5) experiment site (laboratory, field), and (6) toxicant exposure duration. Although aquatic plant organisms play a key role in the functioning of freshwater ecosystems, mainly linked to their primary productivity, their use as biological models in ecotoxicological tests was limited if compared to animals. Also, toxicant effects on freshwater plants were scarcely investigated and limited to studies on microalgae (80%), or only to a certain number of recurrent species (Pseudokirchneriella subcapitata, Chlorella vulgaris, Lemna minor, Myriophyllum spicatum). The most widely tested toxicants on plants were heavy metals (74%), followed by pharmaceutical products and hydrocarbons (7%), while the most commonly utilized endpoints in tests were plant growth inhibition, variations in dry or fresh weight, morpho-structural alterations, chlorosis, and/or necrosis. The main critical issues emerged from plant-based ecotoxicological tests were the narrow range of species and endpoints considered, the lack of environmental relevance, the excessively short exposure times, and the culture media potentially reacting with toxicants. Proposals to overcome these issues are discussed.

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“…Other processes that determine Gly fate are immobilization and leaching: the first one leads to soil adsorption/accumulation, while the second results in water contamination ( Bai and Ogbourne, 2016 ). Gly and AMPA are highly soluble in water and their persistence is variable depending on water conditions with half-lives ranging from few days to several weeks ( Tomlin, 2009 ; Grandcoin et al, 2017 ; ATSDR, 2020 ; Goncalyes et al, 2020 ). In soil, Gly and AMPA accumulate with a discrete persistence with half-lives depending on factors such as pH, salinity, microbial composition, spanning from few days up to about a year ( Bai and Ogbourne, 2016 ; Bento et al, 2016 ; Domínguez et al, 2016 ; Grandcoin et al, 2017 ; ATSDR, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Biological effects of sub-lethal doses of glyphosate and AMPA on cardiac myoblasts

et al. 2023

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Introduction: Glyphosate is the active compound of different non-selective herbicides, being the most used agriculture pesticide worldwide. Glyphosate and AMPA (one of its main metabolites) are common pollutants of water, soil, and food sources such as crops. They can be detected in biological samples from both exposed workers and general population. Despite glyphosate acts as inhibitor of the shikimate pathway, present only in plants and some microorganisms, its safety in mammals is still debated. Acute glyphosate intoxications are correlated to cardiovascular/neuronal damages, but little is known about the effects of the chronic exposure.Methods: We evaluated the direct biological effects of different concentrations of pure glyphosate/AMPA on a rat-derived cell line of cardiomyoblasts (H9c2) in acute (1–2 h) or sub-chronic (24–48 h) settings. We analyzed cell viability/morphology, ROS production and mitochondrial dynamics.Results: Acute exposure to high doses (above 10 mM) of glyphosate and AMPA triggers immediate cytotoxic effects: reduction in cell viability, increased ROS production, morphological alterations and mitochondrial function. When exposed to lower glyphosate concentrations (1 μM—1 mM), H9c2 cells showed only a slight variation in cell viability and ROS production, while mitochondrial dynamic was unvaried. Moreover, the phenotype was completely restored after 48 h of treatment. Surprisingly, the sub-chronic (48 h) treatment with low concentrations (1 μM—1 mM) of AMPA led to a late cytotoxic response, reflected in a reduction in H9c2 viability.Conclusion: The comprehension of the extent of human exposure to these molecules remains pivotal to have a better critical view of the available data.

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Ecotoxicology of Glyphosate-Based Herbicides on Aquatic Environment

Cited by 15 publications

References 89 publications

Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide

Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Biological effects of sub-lethal doses of glyphosate and AMPA on cardiac myoblasts

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