Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato

Bouaziz, Donia; Pirrello, Julien; Amor, Hela Ben; Hammami, Asma; Charfeddine, Mariam; Dhieb, A; Bouzayen, Mondher; Gargouri-Bouzid, Radhia

doi:10.1016/j.plaphy.2012.07.029

Cited by 75 publications

(52 citation statements)

References 60 publications

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“…Results of biochemical analysis indicated significant increase in proline and total free amino acids under salt stress, in agreement with the results of Daneshmand et al (2010), Bouaziz et al (2012) and Marcek et al (2014). The increase in amino acids in potato plantlet under salt stress was also reported by Asensi-Fabado et al (2014).…”

Section: Discussionsupporting

confidence: 87%

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In Vitro Screening of Different Potato Genotypes for Salinity Tolerance

El-Magawry

Mohamed

Elwan

et al. 2015

HJSC

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In vitro screening of several potato genotypes (29 cultivars and lines) to salinity stress was conducted. Single node explants were evaluated on the basis of their growth, microtuberization and biochemical analysis under different concentrations of salt (NaCl) stress. In most cases, increasing NaCl in the tissue culture medium resulted in reduction in plantlet growth, rooting and microtuberization potential in varying degrees, depending on the cultivar. Some genotypes such as Oceania, 97f-267 and Picasso produced higher number of microtuber under 150 mM NaCl than control. Generally, 97f-267, Oceania and Universa genotypes were ranked among salt tolerant, while Nicola, Safran, Diamant were ranked as moderate and Elodi, Triomph, Marabel, Bolista and 99-981 were ranked as salt sensitive based on morphological characters. Photosynthetic pigments decreased under salt stress, however, Universa and Safran maintained higher chlorophyll content under salt conditions (100 mM NaCl) comparing with control (0.0 mM NaCl) plantlets. Also, Universa and Safran accumulated more proline and free amino acids under stress treatment comparing with control, than other tested genotypes. The activities of antioxidant enzymes were different among potato genotypes. In this respect, Universa and Safran had higher catalase (CAT) activity under salt stress (100 mM NaCl) than control treatment (0.0 mM NaCl), however, superoxide dismutase (SOD) activity was not good indicator for salinity tolerance in the tested potato genotypes.

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Section: Discussionsupporting

confidence: 87%

“…Bouaziz et al (2012) and Marcek et al (2014) reported an increase in proline accumulation. Also, Asensia-Fabado et al (2014) found increase in total free amino acids with salinity stress.…”

Section: Introductionmentioning

confidence: 94%

In Vitro Screening of Different Potato Genotypes for Salinity Tolerance

El-Magawry

Mohamed

Elwan

et al. 2015

HJSC

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“…The His-tag fusion protein was purified by Ni-NTA affinity chromatography and the gel mobility shift assays were performed as described previously (Bouaziz et al 2012).…”

Section: Measurement Of Soluble Sugar Contentmentioning

confidence: 99%

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Bouaziz

Jbir

Charfeddine

et al. 2014

Plant Cell Tiss Organ Cult

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We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on the investigation of the StDREB1 target genes by an Electrophoretic mobility shift assay. The data obtains indicated that the StDREB1 protein can bind to both GCC and DRE boxes in the promoter sequence of target genes, suggesting that this transcription factor may play a key role in the response to abiotic-and biotic-stresses by the activation of the DRE-and GCCmediated signaling pathways. In a second step, since some DREB factors were related to the oxidative stress response, we showed that H 2 O 2 treatment led to a significant increase of StDREB1 expression in wild-type potato plants. Moreover, the analysis of the oxidative stress response of StDREB1 transgenic potato plants revealed lower levels of H 2 O 2 and malondialdehyde than wild-type control plants submitted in vitro to salt stress. An increase in the antioxidant enzyme activities including superoxide dismutase (SOD) and catalase was also observed in StDREB1 plants. In addition, an enhanced expression of the Cu/Zn SOD gene was noticed in these StDREB1 transgenic plants, cultivated under salt stress conditions. These results suggest that StDREB1 plays an essential role in the regulation of stress-response by regulating the oxidative stress response. The involvement of this transcription factor in the activation of osmoprotectant synthesis was also confirmed.

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“…We have focused on potato ERF genes because of the economic importance of this plant species worldwide (Bouaziz et al 2012(Bouaziz et al , 2013(Bouaziz et al , 2015Charfeddine et al 2015a, b).…”

Section: Introductionmentioning

confidence: 99%

“…The ectopic expression of these genes in potato conferred enhanced tolerance to salt stress in transgenic plants (Bouaziz et al 2012(Bouaziz et al , 2013.…”

Section: Introductionmentioning

confidence: 99%

Identification and functional characterization of ten AP2/ERF genes in potato

Bouaziz

Charfeddine

Jbir

et al. 2015

Plant Cell Tiss Organ Cult

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Ethylene-responsive element-binding factors (ERF) constitute one of the largest transcription factor families in plants. In this study, we describe the cloning and the characterization of ten cDNAs encoding ERF factors from potato. The alignment of their AP2/ERF (Apetala2/ethylene-responsive factor) domain led to the identification of six StERFs (Solanum tuberosum ERFs) and four StDREBs (dehydration responsive element binding). The phylogeny and the sequence characterization allowed the classification of these StERFs into five ERF families. Expression analysis by semi-quantitative RT-PCR of these genes revealed that most of them are induced by hormone treatment such as abscisic acid, ethephon, jasmonic acid and salicylic acid. However, salt stress induced the expression of all StDREB but only three StERF genes. These results suggest that these transcription factors are involved in salt stress response. The StDREB1 and StDREB2 genes showed strong increase in expression in response to drought stress. In an attempt to improve drought tolerance in potato, we overexpressed StDREB1 and StDREB2 in transgenic potato plants (S. tuberosum L. Group Tuberosum) cv. Belle de Fontenay (BF15) and Spunta, respectively. The level of drought tolerance of these transgenic lines was significantly greater than that of wild-type control plants as measured by relative water content H 2 O 2 content, free proline and total soluble sugars. The results suggest that the StDREB1 and StDREB2 as AP2/ERF transcription factors may play dual roles in response to drought stress in potato.

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Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato

Cited by 75 publications

References 60 publications

In Vitro Screening of Different Potato Genotypes for Salinity Tolerance

In Vitro Screening of Different Potato Genotypes for Salinity Tolerance

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Identification and functional characterization of ten AP2/ERF genes in potato

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