Ectopic nucleolus organizer regions (NORs) in human testicular tumors

DeLozier-Blanchet, C. D.; Walt, Heinrich; Engel, Edgard Eduard

doi:10.1159/000132212

Cited by 39 publications

(4 citation statements)

References 8 publications

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“…Our hypothesis that the distribution and rearrangement of NORs may be essential in tumor evolution is furthermore supported by the detection of ectopic NORs in human testicular tumors by DeLozier-Blanchet et al (1986. The results cited suggest that chromosome aberrations involving NORs could generally be important in tumor initiation and/or propagation.…”

Section: Discussionsupporting

confidence: 58%

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

et al. 1990

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The Hodgkin's lymphoma-derived cell line L540, which exhibits multiple marker chromosomes and a genotype characteristic of T-cell origin, showed expression of MET oncogene. L540 was studied by in situ hybridization for chromosomal rearrangements concerning the regions where genes for TCRA and TCRB chains, for MET oncogene, and for rRNA are located. All four genes were demonstrated to be involved in the formation of marker chromosomes. Especially remarkable was the participation of rDNA-bearing regions in two different translocations.

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Section: Discussionsupporting

confidence: 58%

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

et al. 1990

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“…Nucleolar organizer regions are located on the five acrocentric chromosome pairs; 2 however, they have been reported in abnormal locations (ectopic NOR's) in human testicular tumors. 7 We also examined the expression of NOR's in the chromosomes of cultured cells from human brain tumors.…”

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confidence: 99%

Nucleolar organizer regions in various human brain tumors

Shiraishi¹,

Mineta²,

Momozaki³

et al. 1991

Journal of Neurosurgery

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Nucleolar organizer regions (NOR's) are loops of deoxyribonucleic acid (DNA) which transcribe to ribosomal ribonucleic acid (RNA) by RNA polymerase I. They possess vital significance in the ultimate synthesis of cellular proteins. A silver colloid staining technique for demonstration of NOR-associated proteins (Ag-NOR's) was applied to paraffin-embedded sections from 128 varied brain tumors and to chromosomal preparations from cultured brain-tumor cells. There was a statistically significant difference in the mean number of Ag-NOR's per nucleus between low-grade tumors (1.98/nucleus) and high-grade tumors (2.95/nucleus). It is suggested that the mean number of Ag-NOR's may represent the proliferative potential of brain tumors. Furthermore, high-grade tumors usually showed relatively large Ag-NOR's in a scattered distribution. In chromosomal preparations, the cultured cells displayed five to 12 Ag-NOR's on acrocentric chromosomes. Five of eight cell lines examined demonstrated ectopic Ag-NOR's. This simple staining technique can be easily applied to routinely processed paraffin-embedded sections and will become a useful tool for quick estimation of the proliferative potential of human brain tumors.

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“…In this context, it has been observed that TGCTs as well as GCNIS are characterized by enlarged hyperchromatic nuclei, clumped chromatin and, often, prominent nucleoli. Ectopic NORs have been described in primary germ cell tumors and in teratocarcinoma cell lines that could have been originated by chromosomal rearrangement or by derepression of preexisting inactive NORs [ 82 , 83 ]. All together these features indicate that rRNA synthesis is a potential target process for anti-cancer therapy and that inhibition of Pol I represents an attractive therapeutic approach to block ribosome biogenesis [ 84 ].…”

Section: Non-coding Rnamentioning

confidence: 99%

Non-Coding RNAs and Splicing Activity in Testicular Germ Cell Tumors

Barchi

Bielli

Dolci

et al. 2021

Life

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Testicular germ cell tumors (TGCTs) are the most common tumors in adolescent and young men. Recently, genome-wide studies have made it possible to progress in understanding the molecular mechanisms underlying the development of tumors. It is becoming increasingly clear that aberrant regulation of RNA metabolism can drive tumorigenesis and influence chemotherapeutic response. Notably, the expression of non-coding RNAs as well as specific splice variants is deeply deregulated in human cancers. Since these cancer-related RNA species are considered promising diagnostic, prognostic and therapeutic targets, understanding their function in cancer development is becoming a major challenge. Here, we summarize how the different expression of RNA species repertoire, including non-coding RNAs and protein-coding splicing variants, impacts on TGCTs’ onset and progression and sustains therapeutic resistance. Finally, the role of transcription-associated R-loop misregulation in the maintenance of genomic stability in TGCTs is also discussed.

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Ectopic nucleolus organizer regions (NORs) in human testicular tumors

Cited by 39 publications

References 8 publications

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

Nucleolar organizer regions in various human brain tumors

Non-Coding RNAs and Splicing Activity in Testicular Germ Cell Tumors

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