ECV304 (endothelial) is really T24 (bladder carcinoma): Cell line cross-contamination at source

Dirks, Wilhelm G.; MacLeod, Roderick A.F.; Drexler, Hans G.

doi:10.1007/s11626-999-0091-8

Cited by 127 publications

(74 citation statements)

References 16 publications

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“…11 However, it was shown by DNA fingerprinting that this cell line is a derivative of the human urinary bladder carcinoma cell line T-24. 12 Intracellular extracts containing oligomeric DNA were purified, and pool DNA was amplified by PCR using the terminal constant sequences as primerbinding sites. The resulting selected pool was used for a subsequent cycle of selection for cellular uptake.…”

Section: Resultsmentioning

confidence: 99%

Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment

Lehmann

Sczakiel

2004

Gene Ther

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Section: Resultsmentioning

confidence: 99%

Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment

Lehmann

Sczakiel

2004

Gene Ther

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“…Recent reports revealed genetic similarities between ECV304 and T24, a human bladder epithelial cancer cell line, by comparing their DNA fingerprints. 45,46 Nevertheless, ECV304 shows many endothelial characteristics, resembles Cl-2, a porcine brain microvascular endothelial cells and differs from T24 in phenotype. 47 ECV304 cells not only show endothelial morphology, but also express endothelial markers, uptake Ac-LDL and undergo tube formation in Matrigel.…”

Section: Discussionmentioning

confidence: 99%

Vaccination with xenogeneic tumor endothelial proteins isolated in situ inhibits tumor angiogenesis and spontaneous metastasis

Zhang

Liu

Tan

2009

Intl Journal of Cancer

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Angiogenesis is critical for tumor growth and metastasis. Tumor tissues induce the expression of angiogenesis-associated proteins on endothelial surface that can be targeted for tumor immunotherapy. In our study, the rat tumor endothelial proteins (EP) were isolated in situ via biotinylation of tumor vascular endothelial luminal surface followed by streptavidin affinity chromatography. The isolated tumor EP contained numerous up-regulated angiogenesis-associated endothelial proteins. The administration of these tumor EP as a vaccine to mice reduced the microvessel density in subcutaneous primary LLC tumors, delayed spontaneous LLC tumor metastasis and prolonged post-surgery life span. T lymphocytes from tumor EP-vaccinated mice lysed human umbilical vascular endothelial cells, but not tumor cells in vitro, in a dose-dependent manner. Furthermore, adoptive transfer of antitumor EP antibodies in vivo targeted to tumor endothelium and inhibited spontaneous LLC tumor metastasis. This study provides a successful preclinical exploration of the active immunotherapy for tumor by targeting tumor angiogenesis. ' UICCKey words: endothelium; cancer; dissemination; immunization; biotinylation Angiogenesis, the formation of new blood vessels out of preexisting capillaries by sprouting, is a complex process modulated by the interactions of vascular endothelium with a range of proand antiangiogenic factors. 1 Accumulating evidences show that angiogenesis plays a pivotal role in the progression, invasion and metastasis of solid tumors. 2-4 Antiangiogenesis, originally proposed by Dr. Judah Folkman over 30 years ago, 5 has been considered as a promising strategy to treat cancer by starving the tumors. Various approaches of antiangiogenesis have been developed in recent years, such as endothelial cell inhibitors, extracellular matrix degradation inhibitors, integrin antagonists and angiogenic signaling inhibitors, including specific antibodies targeting vascular endothelial growth factors (VEGFs) or their receptors. 6 However, these antiangiogenesis therapies are relatively expensive and inconvenient, because high doses of therapeutics have to be repeatedly administered for a long period of time because of their rapid clearance in vivo. Moreover, the tumor vasculature marked by highly disorganized anatomical structure, sluggish blood flow and high interstitial pressure reduces effective delivery of therapeutics. 7 Active immunotherapy targeting angiogenesis-related endothelial antigens claims promise overcoming these drawbacks.For vaccine development, researchers have tried various methods to evoke immune responses strong enough to inhibit tumor angiogenesis. The key mediators in the regulation of tumor angiogenesis, VEGFs and their receptors, are the most favorable targets for antiangiogenesis immunotherapy. [8][9][10] Other endothelial membrane proteins involved in angiogenesis such as integrin b3, Tie-2, endoglin and fibroblast growth factor receptor-1 (FGFR-1) have been under preclinical studies and shown therapeutic potenti...

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“…Cell Treatments-The cell line used throughout this study (human bladder carcinoma cells, T24) (25)(26)(27) was the same as that used previously (19) when it was marketed under the code name ECV304. Wild-type CHO cells (CHO-K1) and CHO cells deficient in HSPG synthesis (pgsD-677) were obtained from the American Type Culture Collection.…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide-dependent Processing of Heparan Sulfate in Recycling S-Nitrosylated Glypican-1 Takes Place in Caveolin-1-containing Endosomes

Cheng

Mani

Born

et al. 2002

Journal of Biological Chemistry

108

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However, such residues were scarce in cell surface glypican-1. Brefeldin A-arrested glypican-1, which was non-S-nitrosylated and carried side chains rich in Nunsubstituted glucosamines, colocalized extensively with caveolin-1 but not with Rab9. Suramin, which inhibits heparanase, induced the appearance of Snitrosylated glypican-1 in caveolin-1-rich compartments. Inhibition of deaminative cleavage did not prevent heparanase from generating heparan sulfate oligosaccharides that colocalized strongly with caveolin-1. Growthquiescent cells displayed extensive NO-dependent deaminative cleavage of heparan sulfate-generating anhydromannose-terminating fragments that were partly associated with acidic vesicles. Proliferating cells generated such fragments during polyamine uptake. We conclude that recycling glypican-1 that is associated with caveolin-1-containing endosomes undergoes sequential N-desulfation/N-deacetylation, heparanase cleavage, S-nitrosylation, NO release, and deaminative cleavage of its side chains in conjunction with polyamine uptake.Mammalian glypican-1 (Gpc-1) 1 is a member of a glycosylphosphatidylinositol (GPI)-linked cell-surface proteoglycan (PG) family with six known members to date. These PG, like other cell surface PG, are selective regulators of ligand-receptor encounters and thereby control growth and development (1-4). Gpc proteins are post-translationally modified by the addition of the glycosaminoglycan heparan sulfate (HS) at sites located close to the C-terminal GPI-membrane anchor (see Scheme 1). The central part of the protein consists of a cysteine-rich domain containing information that ensures a high level of HS substitution (5). Many of the functions of Gpc are dependent on the HS side chains, which are capable of binding and/or activating and/or transporting a variety of growth factors, cytokines, enzymes, viral proteins, and polyamines (6 -11).GPI-anchored proteins are usually associated with sphingolipid-and cholesterol-rich plasma membrane domains. Such enriched domains may exist either as small phase-separated "rafts" or, when associated with caveolin-1 (Cav-1), form flaskshaped plasmalemmal invaginations called caveolae, which are involved in signal transduction and special forms of non-clathrindependent endocytosis mediated by Cav-1-containing endosomes, also called caveosomes (12)(13)(14)(15)(16)(17).Biochemical studies using radioactively labeled precursors have demonstrated recycling of newly made Gpc-1 in normal fibroblasts as well as in transformed cells (18,19). During recycling, the HS side chains are degraded both by heparanase and by NO-dependent deaminative cleavage at N-unsubstituted glucosamine residues (GlcNH 3 ϩ ) (20). New HS chains can then be synthesized on the stubs remaining on the core protein (see Scheme 1). Biosynthesis of HS takes place in the Golgi and involves many interacting enzymes (21,22). The stubs should first be extended with a GlcNAc-hexuronic acid (HexUA) repeat backbone. The GlcNH 3 ϩ residues are either a result of inadequate sulfation dur...

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ECV304 (endothelial) is really T24 (bladder carcinoma): Cell line cross-contamination at source

Cited by 127 publications

References 16 publications

Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment

Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment

Vaccination with xenogeneic tumor endothelial proteins isolated in situ inhibits tumor angiogenesis and spontaneous metastasis

Nitric Oxide-dependent Processing of Heparan Sulfate in Recycling S-Nitrosylated Glypican-1 Takes Place in Caveolin-1-containing Endosomes

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