Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice

Wu, Huantong; Yu, Yun; Li, Xixi; Lang, Xiu-Yuan; Gu, Run-Ze; Fan, Sheng-Rui; Fang, Xin; Bai, Jin-Peng; Lan, Rongfeng; Qin, Xiao-Yan

doi:10.1016/j.neuro.2021.05.005

Cited by 14 publications

(6 citation statements)

References 38 publications

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“…Moreover, in the present study, we confirmed that the third investigated compound ED in concentration up to 250 µM was safe for neuronal cells, as was evidenced by cell viability assays and by morphological observations, which was also reported previously in various neuronal and non-neuronal cell types [18,[54][55][56][57]. However, the noted increased level of LDH released to the medium after ED (100 and 250 µM) exposure could be explained by its nonspecific biochemical interaction with the assay rather than biological response (cytotoxic activity).…”

Section: Influence Of the Type Of Experimental Medium On Neuroprotect...supporting

confidence: 91%

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Jantas,

Warszyński,

Lasoń

2023

Molecules

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This study compared the neuroprotective efficacy of three antioxidants—the plant-derived carnosic acid (CA), and two synthetic free radical scavengers: edaravone (ED) and ebselen (EB)—in in vitro models of neuronal cell damage. Results showed that CA protected mouse primary neuronal cell cultures against hydrogen peroxide-induced damage more efficiently than ED or EB. The neuroprotective effects of CA were associated with attenuation of reactive oxygen species level and increased mitochondrial membrane potential but not with a reduction in caspase-3 activity. None of the tested substances was protective against glutamate or oxygen-glucose deprivation-evoked neuronal cell damage, and EB even increased the detrimental effects of these insults. Further experiments using the human neuroblastoma SH-SY5Y cells showed that CA but not ED or EB attenuated the cell damage induced by hydrogen peroxide and that the composition of culture medium is the critical factor in evaluating neuroprotective effects in this model. Our data indicate that the neuroprotective potential of CA, ED, and EB may be revealed in vitro only under specific conditions, with their rather narrow micromolar concentrations, relevant cellular model, type of toxic agent, and exposure time. Nevertheless, of the three compounds tested, CA displayed the most consistent neuroprotective effects.

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Section: Influence Of the Type Of Experimental Medium On Neuroprotect...supporting

confidence: 91%

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Jantas,

Warszyński,

Lasoń

2023

Molecules

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“…D‐galactose/AlCl 3 can cause a series of degenerative changes in cells, including decreased number of nerve cells and abnormal cell morphology (Wu et al, 2021 ). Meanwhile, studies have found that NA intervention can significantly alleviate morphological abnormalities of hippocampus caused by oxidative stress, such as the decreased proportion of pyknosis and apoptosis cells (Vozella et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…In previous reports, it was found that NA intervention can reduce the adverse effects of 6‐hydroxydopamine on PC12 cells, including enhancing the activity of SOD and reducing the production of MDA (Umemoto et al, 2021 ). Meanwhile, NA intervention enhanced GSH‐PX activity in hippocampus and increased neurotransmitter level in normal Kunming mice (Wu et al, 2021 ). In this study, NA enhanced the activity of T‐SOD, GSH‐PX, and CAT, and decreased the content of MDA in liver and brain (Figure 2a ).…”

Section: Discussionmentioning

confidence: 99%

Nervonic acid reduces the cognitive and neurological disturbances induced by combined doses of D‐galactose/AlCl₃ in mice

Aihaiti,

Shi,

Liu

et al. 2023

Food Science & Nutrition

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Nervonic acid (NA) is a kind of ultra‐long‐chain monounsaturated fatty acid, which can repair nerve cell damage caused by oxidative stress. Alzheimer's disease (AD) is a nervous system disease and often accompanied by the decline of learning and memory capacity. In this study, the combined dose of D‐galactose/AlCl3 was used to establish a mouse model of AD. Meanwhile, the mice were treated with different doses of NA (10.95 and 43.93 mg/kg). The results showed that NA delayed the decline of locomotion and learning ability caused by D‐galactose/AlCl3, increased the activity of total superoxide dismutase, catalase, glutathione peroxidase, and reduced the content of malondialdehyde in vivo. Besides, NA reduced the levels of interleukin‐6 (IL‐6), tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), aspartate aminotransferase, alanine aminotransferase, increased the levels of 5‐hydroxytryptamine, dopamine, γ‐aminobutyric acid, alleviated the cell morphology damage induced by D‐galactose/AlCl3 in hippocampus and liver tissue. Furthermore, the intervention of NA upregulated the expression levels of PI3K, AKT, and mTOR genes and downregulated the expression levels of TNF‐α, IL‐6, and IL‐1β genes. Therefore, we speculate the intervention of NA could be an effective way in improving cognitive impairment through the activation of PI3K signaling pathway. These results suggest that NA has the potential to be developed as antioxidant drug for the prevention and early therapy of AD.

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“…As previously reported, GSH deficiency and IL-1 β administration can impair the expressions of BDNF in the hippocampus of rats [ 37 , 38 ]. However, BNDF expressions can be promoted by free radical scavenging molecules (such as vitamin E [ 39 ], bikaverin [ 40 ], rosmarinic acid [ 41 ], and edaravone [ 42 ]) and anti-inflammatory ingredients (such as doxycycline [ 43 ] and minocycline [ 44 ]). Especially, many plant-derived polyphenols with strong antioxidant and anti-inflammatory abilities, including quercetin [ 45 ], resveratrol [ 46 ], apigenin [ 47 ], and naringenin [ 48 ], can increase BDNF levels in several animal models of neurological disorders.…”

Section: Discussionmentioning

confidence: 99%

Blueberry Anthocyanins Extract Attenuates Acrylamide-Induced Oxidative Stress and Neuroinflammation in Rats

Fang

Luo

et al. 2022

Oxidative Medicine and Cellular Longevity

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Acrylamide (AA) is a widespread environmental and dietary-derived neurotoxin, which can induce oxidative stress and associated inflammation in the brain. Anthocyanins widely occur as natural antioxidant and anti-inflammatory phytochemicals. Herein, the protective effects of blueberry anthocyanins extract (BAE) against AA-induced neurotoxicity were investigated in rats. The rats were pretreated with BAE (175 mg/kg body weight/day) by oral gavage for the first 7 days, followed by the co-administration of BAE and AA (35 mg/kg body weight/day) by oral gavage for the next 12 days. Results showed that BAE significantly decreased the malondialdehyde (MDA) production, and increased glutathione (GSH) and antioxidant enzyme levels; and it also suppressed microglial activation, astrocytic reaction, and pro-inflammatory cytokine expressions. Furthermore, BAE elevated the extracellular signal-related kinase (ERK)/cAMP response elements binding protein (CREB)/brain-derived neurotrophic factor (BDNF) pathway, and relieved the accumulation of amyloid beta (Aβ) 1-42 and 1-40 after AA exposure. Consequently, AA-induced neuronal necrosis and downregulation of synaptosomal-associated protein 25 (SNAP-25) were attenuated by BAE in the hippocampus and cerebral cortex. In conclusion, BAE can exert a protective function on neurons and synapses against AA-induced oxidative stress and neuroinflammation.

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Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice

Cited by 14 publications

References 38 publications

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Nervonic acid reduces the cognitive and neurological disturbances induced by combined doses of D‐galactose/AlCl₃ in mice

Blueberry Anthocyanins Extract Attenuates Acrylamide-Induced Oxidative Stress and Neuroinflammation in Rats

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Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice

Cited by 14 publications

References 38 publications

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Carnosic Acid Shows Higher Neuroprotective Efficiency than Edaravone or Ebselen in In Vitro Models of Neuronal Cell Damage

Nervonic acid reduces the cognitive and neurological disturbances induced by combined doses of D‐galactose/AlCl3 in mice

Blueberry Anthocyanins Extract Attenuates Acrylamide-Induced Oxidative Stress and Neuroinflammation in Rats

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Nervonic acid reduces the cognitive and neurological disturbances induced by combined doses of D‐galactose/AlCl₃ in mice