Edible Ginger-derived Nano-lipids Loaded with Doxorubicin as a Novel Drug-delivery Approach for Colon Cancer Therapy

Abstract

The use of nanotechnology for drug delivery has shown great promise for improving cancer treatment. However, potential toxicity, hazardous environmental effects, issues with large-scale production, and potential excessive costs are challenges that confront their further clinical applications. Here, we describe a nanovector made from ginger-derived lipids that can serve as a delivery platform for the therapeutic agent doxorubicin (Dox) to treat colon cancer. We created nanoparticles from ginger and reassembled their lipids into ginger-derived nanovectors (GDNVs). A subsequent characterization showed that GDNVs were efficiently taken up by colon cancer cells. Viability and apoptosis assays and electric cell-substrate impedance-sensing technology revealed that GDNVs exhibited excellent biocompatibility up to 200 μmol/l; by contrast, cationic liposomes at the same concentrations decreased cell proliferation and increased apoptosis. GDNVs were capable of loading Dox with high efficiency and showed a better pH-dependent drug-release profile than commercially available liposomal-Dox. Modified GDNVs conjugated with the targeting ligand folic acid mediated targeted delivery of Dox to Colon-26 tumors in vivo and enhanced the chemotherapeutic inhibition of tumor growth compared with free drug. Current experiments explore the feasibility of producing nature-derived nanoparticles that are effective as a treatment vehicle while potentially attenuating the issues related to traditional synthetic nanoparticles.

Assessment of Ginger Extract and Ginger Nanoparticles Protective Activity against Acetaminophen-Induced Hepatotoxicity and Nephrotoxicity in Rats

“…Lately, natural nanoparticles had been fabricated from plants, characterized by easy preparation for large scale relative to synthetic nanoparticles (Zhang et al, 2016). Natural nanoparticles from edible plants can distribute from intestine to the liver, thus they may have an important role in treatment regimen and delivery of drug to the target tissues and improvement of therapeutic results.…”

<p>Nanoparticle-Mediated Drug Delivery Systems For The Treatment Of IBD: Current Perspectives</p>

“…Orally administrated GDNPs were found to efficiently target the colon and were mainly absorbed by cells in the lining of the intestine, which is the site of IBD-associated inflammation. 98 The colon-targeting function of GDNPs is multi-factorial and is likely to involve size-, ligand-, and receptor-mediated actions. The capacity of other plant-derived NPs, including grape-, grapefruit-, and broccoli-derived NPs, to target different tissues is also likely to be mediated via multiple factors.…”

Ginger: Panacea or Consumer’s Hype?

“…These new carriers are taken up by intestinal cells, as demonstrated in vitro on the Colon-26 and HT-29 human colon adenocarcinoma cell lines, and biocompatible as demonstrated by the lack of toxicity in vivo on mice. Furthermore, they act effectively as carriers for oral administration of the antitumoral drug doxorubicin and this therapy successfully inhibited tumor growth in a Colon-26 xenograft tumor mouse model [97].…”