Editorial: Targeting the endocannabinoidome in neurodegenerative disorders

Ishiguro, Hiroki

doi:10.3389/fnagi.2022.1116635

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…The discovery of the ECS revolutionized our understanding of how cannabinoids, both external and internal, interact with the human body. This regulatory system influences a wide range of functions, including pain modulation, appetite, mood regulation, sleep, memory, the immune response and inflammation ( 1 , 2 ).…”

Section: Introductionmentioning

confidence: 99%

Anandamide modulation of monocyte-derived Langerhans cells: implications for immune homeostasis and skin inflammation

Pénzes,

Horváth,

Molnár

et al. 2024

Front. Immunol.

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IntroductionThe endocannabinoid system (ECS), named after the chemical compounds found in the cannabis plant, is a regulatory network of neurotransmitters, receptors, and enzymes that plays crucial roles in skin health and disease. Endogenous ligands of the ECS, called endocannabinoids, have proven to be important regulators of immune responses. One of the most prevalent endocannabinoids, arachidonoylethanolamide (also known as anandamide), is known for its anti-inflammatory effects. Langerhans cells (LCs) are the sole antigen-presenting cells present in the human epidermis. They serve as the first line of defense against pathogens and are essential for the skin’s specific immune responses and play a critical role in maintaining tissue homeostasis; however, little is known about the effect of endocannabinoids on these cells. Our research aimed to provide the connection between monocyte-derived Langerhans cells (moLCs) and the ECS, shedding light on their collaborative roles in immune homeostasis and inflammation.MethodsHuman monocytes were differentiated into moLCs using established protocols. Anandamide was applied during the differentiation process to test its effect on the viability, marker expression, and cytokine production of the cells, as well as in short term treatments for intracellular calcium measurement. TLR ligands applied after the differentiation protocol were used to activate moLCs. The impact of anandamide on the functionality of moLCs was further assessed using differential gene expression analysis of bulk RNA-Seq data, moLC-T cell cocultures, while ELISpot was employed to determine polarization of T cells activated in the aforementioned cocultures.ResultsAnandamide did not significantly affect the viability of moLCs up to 10 µM. When applied during the differentiation process it had only a negligible effect on CD207 expression, the prototypic marker of LCs; however, there was an observed reduction in CD1a expression by moLCs. Anandamide had no significant effects on the maturation status of moLCs, nor did it affect the maturation induced by TLR3 and TLR7/8 agonists. MoLCs differentiated in the presence of anandamide did however show decreased production of CXCL8, IL-6, IL-10 and IL-12 cytokines induced by TLR3 and TLR7/8 activation. Anandamide-treated moLCs showed an increased capability to activate naïve T cells; however, not to the level seen with combined TLR agonism. RNA sequencing analysis of moLCs differentiated with anandamide showed modest changes compared to control cells but did reveal an inhibitory effect on oxidative phosphorylation specifically in activated moLCs. Anandamide also promoted the polarization of naïve T cells towards a Th1 phenotype.DiscussionOur results show that anandamide has nuanced effects on the differentiation, maturation, cytokine secretion, metabolism and function of activated moLCs. Among these changes the decrease in CD1a expression on moLCs holds promise to selectively dampen inflammation induced by CD1a restricted T cells, which have been implicated as drivers of inflammation in common inflammatory skin conditions such as psoriasis, atopic dermatitis and contact dermatitis.

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Section: Introductionmentioning

confidence: 99%

Anandamide modulation of monocyte-derived Langerhans cells: implications for immune homeostasis and skin inflammation

Pénzes,

Horváth,

Molnár

et al. 2024

Front. Immunol.

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“…While there exists some controversy, ambiguity, and lingering debate about the functional neuronal expression of CB2Rs, a number of studies demonstrate that CB2Rs are expressed at low basal levels in the CNS, but that they are dynamic, inducible, and can be upregulated in neuropsychiatric and neurological disorders ( 2 , 3 , 8 ). This provides a potential role of CB2Rs in neuropsychiatric and neurodegenerative disorders, as CB1R ligands are associated with CNS side effects.…”

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confidence: 99%