2018
DOI: 10.1128/mbio.00306-18
|View full text |Cite
|
Sign up to set email alerts
|

EF-P Posttranslational Modification Has Variable Impact on Polyproline Translation in Bacillus subtilis

Abstract: Elongation factor P (EF-P) is a ubiquitous translation factor that facilitates translation of polyproline motifs. In order to perform this function, EF-P generally requires posttranslational modification (PTM) on a conserved residue. Although the position of the modification is highly conserved, the structure can vary widely between organisms. In Bacillus subtilis, EF-P is modified at Lys32 with a 5-aminopentanol moiety. Here, we use a forward genetic screen to identify genes involved in 5-aminopentanolylation… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

1
35
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 32 publications
(36 citation statements)
references
References 29 publications
1
35
0
Order By: Relevance
“…The function of both EF-P and e/aIF-5A in assisting polyproline translation depends on posttranslational modification (8,14). In this regard, EF-P can be ␤-lysylated (15)(16)(17), 5-aminopentolylated (18)(19)(20), and rhamnosylated (7,21,22). The eukaryotic/archaeal ortholog e/aIF5A, in contrast, strictly depends on (deoxy)hypusination (23).…”
mentioning
confidence: 99%
“…The function of both EF-P and e/aIF-5A in assisting polyproline translation depends on posttranslational modification (8,14). In this regard, EF-P can be ␤-lysylated (15)(16)(17), 5-aminopentolylated (18)(19)(20), and rhamnosylated (7,21,22). The eukaryotic/archaeal ortholog e/aIF5A, in contrast, strictly depends on (deoxy)hypusination (23).…”
mentioning
confidence: 99%
“…Modification status of other bacteria is unknown. Highly possible modification of S.aureus EF-P could be 5-aminopentanolitation of 34 K, the same type modification as for B.subtillus EF-P [15].…”
Section: Introductionmentioning
confidence: 99%
“…Therefore the knowledge about dynamics of the loop I could be crucial for understanding of EF-P interaction process with P-tRNA near PTC and the function of modifications. Recently we analyzed a dynamic of SaEF-P backbone amide protons by high-resolution NMR spectroscopy [17] and due to high mobility of the loop between β-sheets β2 and β3 (residues 30-34) the amide protons have not been detected in 15 N-1 H heteronuclear NMR spectra due to fast proton exchange leading to the absence of the corresponding amide resonances. Site-directed spin labeling in concert with electron paramagnetic resonance (EPR) spectroscopy is a powerful method for studying the nature of proteins in solution which has been especially useful for examining proteins that are large, flexible or highly dynamic [18][19][20].…”
Section: Introductionmentioning
confidence: 99%
“…While EF-P ␤-lysylation was originally described in enterobacteria, it is now clear that several other posttranslational modification pathways exist in more divergent bacteria. A lysine residue is posttranslationally modified in Bacillus subtilis by a range of enzymes (15). In Pseudomonas aeruginosa, EF-P is instead rhamnosylated at a conserved arginine residue by EarP (16).…”
mentioning
confidence: 99%
“…In Pseudomonas aeruginosa, EF-P is instead rhamnosylated at a conserved arginine residue by EarP (16). Unlike most species in which mutations to modifying enzymes produce a similar phenotype to efp mutants, B. subtilis-modifying enzyme mutants show distinct phenotypes, including altered proline translation rate and sensitivity to certain chemicals (15).…”
mentioning
confidence: 99%