Efecto gastroprotector y antisecretor de un fitofármaco de hojas de matico (Piper aduncum)

Abstract

Objetivos. Determinar el efecto gastroprotector y antisecretor del extracto etanólico de las hojas de matico (Piper aduncum) en modelos animales. Materiales y métodos. Para la evaluación del efecto gastroprotector se utilizó 220 ratones de la cepa Balb C57, los cuales fueron aleatorizados en 22 grupos de diez animales, a los cuales se les indujo la formación de úlceras gástricas con indometacina, la gastroprotección se determinó a través de tres aspectos: inflamación, número de bandas hemorrágicas y número de úlceras. Para evaluar el efecto antisecretor se utilizó 64 ratas albinas machos Holtzman, los cuales fueron aleatorizados en ocho grupos de ocho animales, un control y siete grupos de tratamiento con un nivel de dosis de los extractos y dos niveles de dosis en los fitofármacos; la antisecreción se realizó con el ensayo de ligazón pilórica. Resultados. Para la gastroprotección, los extractos de diclorometano, cloroformo, hexano y metanol, lograron una disminución de la inflamación de más del 66% (p<0,05); el extracto etanólico presenta una actividad de 100% para disminuir el número de bandas hemorrágicas (p<0,05); el extracto clorofórmico presenta una actividad antiulcerosa de 75% (p<0,05). Respecto a la antisecreción, el fitofármaco en cápsulas conteniendo el extracto etanólico logró un 72% de reducción del volumen de la secreción gástrica (p<0,01) y un incremento del pH en 104,3% (p<0,01). Conclusiones. En condiciones experimentales los extractos etanólico, sus fracciones y su fitofármaco son gastroprotectores en ratones y antisecretores en ratas. Palabras clave: Matico; Plantas medicinales; Fitoterapia; Extractos vegetales (fuente: DeCS BIREME). GASTROPROTECTIVE AND ANTISECRETORY EFFECT OF A PHYTOCHEMICAL MADE FROM MATICO LEAVES (Piper aduncum)ABSTRACT Objectives. To determine the gastroprotective and antisecretory effect of ethanol extract from matico leaves (Piper aduncum) in animal models. Materials and methods. To evaluate the gastroprotective effect, 220 mice of the Balb C57 strain were used. They were randomized in 22 groups of ten animals each, in which the formation of gastric ulcers was induced with indomethacin. Gastroprotection was determined by evaluating three aspects: inflammation, number of hemorrhagic shocks and number of ulcers. To evaluate the antisecretory effect, 64 white male Holtzman rats were used, which were randomized in eight groups of eight animals, one control and seven groups of treatment with one extract dose level and two phytochemical dose levels. Antisecretion was obtained through the pylorus ligation. Results. Regarding gastroprotection, dichloromethane, chloroform, hexane and methanol extracts decreased inflammation to over 66% (p<0,05). The ethanolic extract shows 100% activity in reducing the number of hemorrhagic bands (p<0,05). The chloroform extract shows antiulcer activity at 75% (p<0,05). In terms of antisecretion, the phytochemical in capsules containing the ethanolic extract achieved 72% reduction of the gastric secretion volume (p<0,01) and 104,3% (p<0,01) PH i...

D‐limonene: A multifunctional compound with potent therapeutic effects

“…Extensive research is carried out to determine the gastroprotective efficacy of numerous flavonoids, alkaloids, and related compounds from plants. These compounds are thought to be safe for usage (Arroyo et al, 2013). Role of terpenes as gastroprotective agents have been studied (Areche et al, 2009).…”

Inhibition of Urease, Elastase, and β-Glucuronidase Enzymatic Activity by Applying Aqueous Extracts of Opuntia oligacantha C.F. Först Acid Fruits: In Vitro Essay under Simulated Digestive Conditions

“…The inhibition that xoconostle turned out to have on elastase is probably part of the action of the tannins contained in it, since these are considered bioactive compounds with anti-oxidant and anti-inflammatory properties due to their ability to inhibit specific enzymes and their destructive activity of free radicals, which is why they can stop some of the anti-inflammatory processes [31]. In a study carried out by Arroyo et al [35], it was found that the methanolic and ethanolic extract of the leaves of matico (Piper aduncum) in animal models achieved a decrease in inflammation of more than 66% (p < 0.05) due to the presence of phenolic compounds, flavonoids, tannins, saponins, and alkaloids, since these (mainly flavonoids) can inactivate cellular enzymes that cause changes in the permeability of the membrane. As elastase is a proteolytic enzyme related to inflammatory diseases of the epithelial tissue, [5,6] explain that inflammatory cells such as macrophages and neutrophils, in the presence of toxic agents, can secrete various protease enzymes such as tryptase, chymase, and elastase, capable of degrading components of the extracellular matrix that amplify inflammation and free radicals.…”

Total Phenol Content and Gastric Anti-Ulcer Activity of Hydroalcoholic Extract of Persea caerulea (Ruiz & Pav.) Mez. Bark

“…of physiological saline solution (PSS); 24 hours of fasting: 1st induction with 0.5 mL of PSS + 0.5 mL of PSS; 12 hours later: 2nd induction with 0.5 mL of PSS + 0. For five consecutive days, gastroprotective treatments were administered to the mice, then they were fasted for 24 hours; After this time, the first induction of ulceration was carried out with 50 mg/Kg of indomethacin; 12 hours later the first gastric ulcer induction, the second dose of indomethacin (50 mg/Kg) was administered; 60 minutes after indomethacin administration, the gastroprotective substance [11][12] was administered. After 6 hours of this last dosage, euthanasia was carried out with a dose of 60 mg/kg i.p.…”