Efeitos do estádio de desenvolvimento da antera e da radiação gama na formação de calos derivados de anteras de tomate

Brasileiro, Ana Christina R.; Willadino, Lília; Guerra, Marcelo; Colaço, Waldeciro; Meunier, Isabelle Maria Jacqueline; Câmara, Terezinha Rangel

doi:10.1590/s0103-90161999000400010

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…Anthers containing microspores, obtained from buds with length ranging from 5.0 to 5.9 mm, did not respond to in vitro induction. Other studies corroborated that meiocytes is the most responsive stage for tomato anther culture (Zamir et al, 1980;Summers et al, 1992;Brasileiro et al, 1999;Seguí-Simarro and Nuez, 2005). Gresshoff and Doy (1972), Summers et al (1992) and Brasileiro et al (1999) reported that anthers showed higher frequencies of callus production during prophase I.…”

Section: Discussionmentioning

confidence: 61%

“…In this study, we show that flow cytometry can be a useful tool to screen the developmental stage of anthers. Brasileiro et al (1999) also analyzed the correlation between tomato anther length and meiotic phase. These authors reported that flower buds of size 5.0 to 6.0 mm and anthers of size 2.0 to 2.3 mm showed cells in meiosis.…”

Section: Discussionmentioning

confidence: 99%

“…We have identified the response of four different tomato genotypes with different anther sizes in relation to calli production that were induced in an induction medium previously tested. Most studies on tomato anther culture have reported the occurrence of callogenesis ((Zamir et al, 1980;Shtereva et al, 1998;Brasileiro et al, 1999;Seguí-Simarro and Nuez, 2007;Corral-Martínez et al, 2005;2011). However, considering the difficulty in achieving plant regeneration from calli in the test species besides the high recalcitrance of tomato to another culture (Seguí-Simarro and Nuez, 2005;Corral-Martínez et al, 2011), very little progress has been made in this context, with no standardized method being available for the generation of haploid and DH tomato plants (Seguí-Simarro and Nuez, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…In nearly all responsive crops, including model species such as rapeseed and tobacco, the inducible stage revolves around the first pollen mitosis, that is, from vacuolate microspore to early bicellular pollen (Touraev et al, 2001). In most tomato, the meiocyte is the most suitable developmental stage for androgenesis induction (Zamir et al, 1980;Summers et al, 1992;Shtereva et al, 1998;Brasileiro et al, 1999;Seguí-Simarro and Nuez, 2005;. However, according to Seguí-Simarro and Nuez (2005), the stage with highest response in terms of callus generation in tomato is metaphase I to telophase II.…”

Section: Introductionmentioning

confidence: 99%

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Multiploidy occurrence in tomato calli from anther culture

Julião¹,

Carvalho

Silva³

et al. 2015

Afr. J. Biotechnol.

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Anther culture has long been used for the production of fully homozygous lines in order to produce, mainly, doubled haploid plants, which are of great interest in plant breeding. For tomato, a recalcitrant species for androgenesis production protocols have not been standardized. It is known that the genotype, anther size, the developmental stage of the microspore, and the medium composition are some factors that can influence the calli production. The present study aimed to adapt flow cytometry methodology to verify the microsporogenesis phases of anthers in order to assess the anther responsiveness of different tomato genotypes in an androgenesis-induction culture medium and to analyze the DNA ploidy level of calli produced by flow cytometry. Anthers from flower buds of length 1.0 to 5.9 mm, corresponding to the size range as analyzed by flow cytometry and cytogenetic methods, were inoculated into Murashige and Skoog (MS) basal medium containing the growth regulators 6-(y,ydimethylallylamino) purine and indole-3-acetic acid. The obtained calli were subsequently analyzed by flow cytometry to determine the DNA ploidy level. Surprisingly, despite no pretreatment with microtubule-depolymerizing agents, five classes of multiploid calli were observed, as follows: class I (2C-4C-8C-16C), class II (2C-4C-8C-16C-32C), class III (4C-8C), class IV (4C-8C-16C) and class V (8C-16C-32C). Multiploid calli were identified in short-term (two month) culturing, suggesting that the variable culture duration did not directly influence the occurrence of endoreduplication. In this work, this type of somaclonal variation has been reported for the first time in tomato anther culture, and their possible origin has been discussed.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiploidy occurrence in tomato calli from anther culture

Julião¹,

Carvalho

Silva³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

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“…For many crops, the stage near to the first mitosis has been considered the most inductive (Peters et al, 1999). Secale cereale (Wang et al, 2000) and Brassica oleraceae (Kameya and Hinata, 1970) are exceptions in which the pollen grains must be with the starch deposition and Arabidopsis thaliana (Gresshoft and Doy, 1972) and Lycopersicon escutelum (Brasileiro et al, 1999) that must be at meiosis. For cereals the optimal developmental stages are mid to late uninucleate, in barley and wheat, and late uninucleated and early binucleated for rice and maize (Jähne and Lörz, 1995).…”

Section: Introductionmentioning

confidence: 99%

Morphological markers for microspore developmental stage in maize

Moraes

Bered

Carvalho

et al. 2008

Braz. arch. biol. technol.

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Haploidy in tomato (Lycopersicon esculentum Mill.): a critical review

Bal

Abak

2007

Euphytica

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Results on the induction of haploidy in tomato via both gynogenesis and microspore embryogenesis in vitro are far from satisfactory. The number of reports available on the gynogenic induction via in vitro non-fertilized ovary culture, wide hybridization and the use of irradiated pollen are limited. The main reason for this may be the difficulty experienced in working with this species. Therefore, many failed attempts have not been reported. Non-fertilized ovary culture and wide hybridization using Solanum sisymbriifolium Lam. as the male parent seem to be promising (Bal and Abak, Pak J Biol Sci 6: 745-749, 2003a, b). Further efforts in this line may improve results obtained earlier. available on anther culture of tomato but a working protocol is yet to be developed. For the induction of anther callus, anthers carrying microspores at the meiotic stages appear to be the most responsive. However, the callus and the regenerants obtained were mainly of somatic origin. Somatic tissues of tomato anthers carrying the meiotic stages are highly responsive to tissue culture manipulations in comparison to anther tissues of the later stages. Therefore, reports on the induction of callus from anthers carrying early microspore stages should be met with caution. If culturing young anthers is of any help then it may be that the anther tissues are nursing the microspores and bringing them to the responsive uninucleate stage. Following the first report by Sharp et al. (Planta 104:357-361, 1972) on the induction of microspore embryogenesis, using a modified version of the microspore culture, reports concentrated only on anther culture (reviewed by Chlyah et al., Haploids in crop improvement I. Biotechnology in agriculture and forestry 12. Springer-Verlag, Berlin, 1990). Based on findings reported by Yinnan et al. (J Agric Biotechnol, http://www.cau.edu.cn/ agrocbi/periodical/ nyswjsxb/ nysw99/ nysw9901/ 990115.htm, 1999) and Bal and Abak (Biotechnol Biotechnol Equip 19:35-42, 2005) on the induction of symmetrical division of microspore nuclei from uninucleate microspores, the formation of multicellular structures and globular embryos, it is likely that the future of tomato haploidy lies in the technique of isolated microspore culture.

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Efeitos do estádio de desenvolvimento da antera e da radiação gama na formação de calos derivados de anteras de tomate

Cited by 5 publications

References 17 publications

Multiploidy occurrence in tomato calli from anther culture

Multiploidy occurrence in tomato calli from anther culture

Morphological markers for microspore developmental stage in maize

Haploidy in tomato (Lycopersicon esculentum Mill.): a critical review

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