Efek Pemberian Tepung Kedelai terhadap Gambaran Histopatologi Ginjal Tikus Wistar yang Diinduksi Diazinon

Qodar, Tegar Syaiful; Wisudanti, Desie Dwi; Aziz, Ayu Munawaroh

doi:10.23886/ejki.7.10287.

Cited by 4 publications

(7 citation statements)

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“…This result was in accordance with a previous study on the nephroprotective effect of soy flour on the histopathological change in rat kidney induced by diazinon. (19) The aforementioned study proved that soy flour supplementation at a dose of 1 g/mL, 1.5 g/mL and 2 g/mL for 28 days had a significantly lower histopathological score compared to the group without diazinon. The group on 2 g/mL soy flour had the lowest renal histopathological score, approaching the normal control group score.…”

Section: Discussionmentioning

confidence: 84%

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

2020

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Background Diazinon is a widely used pesticide to eradicate agricultural pests globally with the potential to cause harmful effects on humans by generating free radicals and depleting endogenous antioxidants. Soy flour possesses high antioxidant effect. This study evaluated the nephroprotective effect of soy flour on diazinon-induced nephrotoxicity in rats. Methods An experimental laboratory study was carried out involving 30 male Wistar rats were randomly divided into five groups of 6 rats. The normal control (Kn) and the negative control (K-) groups received normal saline, the treatment groups (K1, K2, K3) received 10%, 15%, and 20% soy flour in distilled water, respectively, on day 1 to day 28. Subsequently, for five days, all groups received diazinon 40 mg/kg, except for the normal control group. Blood urea nitrogen (BUN) and creatinine levels were measured at the end of the study. The Kruskal Wallis test with the post hoc Mann Whitney U test were used to analyze the data. Results Blood urea nitrogen and creatinine levels were lower in all treatment groups (K1, K2, K3) compared to the negative control group (K-), while the K3 level was the lowest (24.28 ± 1.56 mg/dL and 0.93 ± 0.14 mg/dL). There was a significant decrease in BUN and creatinine levels in the experimental rats of the 15% and 20% soy flour groups (K2, K3) compared to the negative control group (K-). Conclusion Administration of soy flour (Glycine max (L.) Merr.) had a nephroprotective effect in preventing increases in BUN and creatinine levels of diazinon-induced nephrotoxic male Wistar rats.

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Section: Discussionmentioning

confidence: 84%

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

2020

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“…Tissue fragmentation and fragility due to increased MDA and cell death due to apoptosis can cause hepatocellular damage, which then manifests as increased AST and ALT levels and histopathological changes. 16,23,25 The results of the histopathological structure observations in the K1, K2, and K3 groups showed a lower average score than in the K(-) group. These results indicate the hepatocellular mechanism of protecting soy flour content.…”

Section: Resultsmentioning

confidence: 93%

“…Soy flour which contains isoflavones, especially genistein and daidzein, can act as a scavenger against free radical agents. 25,26 Daidzein and genistein act as a hydrogen atom transfer (HAT) through hydrogen atom donors, and as a single electron transfer (SET) through electron transfer to reduce metal ions, radicals, and carbonyl. The role of these compounds in counteracting free radicals is through preventing the initiation of fat peroxidation through its reaction with radical lipids.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotective Effects of Soy Flour (Glycine max (L.) Merr.) Supplementation in Diazinon-Treated Wistar Rats

2021

TJNPR

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Insecticide has been used as an effort to optimize effectiveness in agriculture. Diazinon is one of the most commonly used insecticides, widely used by farmers to eradicate pests in their agricultural environment. 1 In general, it eradicates pests through inhibition of the Acetylcholinesterase (AChE) enzyme. 2,3 Diazinon, a type of organophosphate, can induce toxicity in the target (plant pests) and non-target organisms (humans). 2 Diazinon causes toxicity through the induction of oxidative stress and apoptotic mechanisms. 1 Diazoxon is a result of diazinon metabolism modulated by cytochrome P450 (CYP450) in liver tissue. These compounds are considered to be oxidative stress inducers because they increase the production of Reactive Oxygen Species (ROS), which bind to proteins and lipids to initiate lipid peroxidation and contribute to liver damage. 3,4 Several studies stated that the administration of diazinon at several doses has a hepatotoxic effect. It is indicated by changes in histopathological structure, increased levels of liver biochemistry; Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline

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“…Slides were photographed using a Leica LAS EZ (Leica Microsystem ® ) and histopathological assessment was carried out. The observed damage criteria are in accordance with Table 1 according to the scoring method of Qodar et al (2019).…”

Section: Histopathological Examinationmentioning

confidence: 99%

In Vivo Evaluation of Heavy Metal Decontaminated Marine Macroalgae (Ulva lactuca L.) Using Bilimbi (Averrhoa bilimbi) Juice and Its Potency As Nephroprotector

Hasnadewi,

Mulyati,

Nuriliani

et al. 2023

IFNP

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Ulva lactuca is a functional food ingredient with high antioxidant content. The presence of heavy metal contaminants in natural Ulva lactuca needs to be eliminated. Ulva lactuca was investigated for its potential as a nephroprotector in female Wistar rats. The study assessed Blood Urea Nitrogen (BUN) levels, creatinine levels, and kidney histopathology. The experiment included a Group I (control), Group II (administered 1000 mg/Kg BW/day of natural Ulva lactuca), and Group III (administered 1000 mg/Kg BW/day of heavy metal decontaminated Ulva lactuca using bilimbi juice). After 30 days of oral treatment, no significant differences were observed in BUN levels before and after treatment. However, significant variations were found in Group III compared to the Group I creatinine levels after treatment. Both natural and heavy metal decontaminated Ulva lactuca showed no adverse effects on kidney function. Notably, heavy metal decontaminated Ulva lactuca exhibited improved kidney histopathology, suggesting its potential as a nephroprotector. Further research is needed to explore its broader applications in promoting kidney health.

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Efek Pemberian Tepung Kedelai terhadap Gambaran Histopatologi Ginjal Tikus Wistar yang Diinduksi Diazinon

Cited by 4 publications

References 0 publications

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

Hepatoprotective Effects of Soy Flour (Glycine max (L.) Merr.) Supplementation in Diazinon-Treated Wistar Rats

In Vivo Evaluation of Heavy Metal Decontaminated Marine Macroalgae (Ulva lactuca L.) Using Bilimbi (Averrhoa bilimbi) Juice and Its Potency As Nephroprotector

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