EFEKTIFITAS EKSTRAK DAUN KELOR (Moringa oleifera lam) DALAM MEMPERBAIKI PROFIL SERUM IRON, SATURASI TRANSFERIN, DAN TOTAL IRON BINDING CAPACITY TIKUS PUTIH JANTAN DIET RENDAH ZAT BESI

Abstract

Background : Iron deficinecy can cause anemia which affect health and work productivity. Moringa oleifera leaves contain a lot of iron and vitamin C, are thought to increase serum iron levels, transferin saturation, and improve total iron binding capacity.Objectives : To determine the effectiveness of Moringa oleifera lam. leaf extract on serum iron levels, transferin saturation, and total iron binding capacity in white rats (Rattus novergicus strain wistar) given a diet low in iron (Fe).Materials and methods : true experimental research using post test only control group design. The object of theis study was thirty male white mice (Rattus novergicus strain wistar) who were given a low iron diet. The study was divided into negative control group, positive control, treatment groups with a dose of Moringa leaf extract 400 mg/kg BW, 800 mg/kgBW, 1,600 mg/kg/BW, an the ferro fumaras control group (FF) 175 mg/200 BW.Results and discussion : Multivariate manova serum iron 0.001 (p<0.05), TBC 0.19 (P>0.05), and transferin saturation 0.001(p<0.05). In the post Hoc Tukey the mean serum iron and transferin saturation appeared to differ significant in the negative control group with all the other treatment groups, but did not show a significant diffrence beetwen the positive control group, FF Group, and the treatment groups.Conclusion : The adminstration of Moringa oleifera leaf extractwas less significant in improving serum iron levels, transferin saturation, and total iron binding capacity of male white mice induced by a low iron diet.

Pengaruh Pemberian Pakan Rendah Besi Dan Induksi Natrium Nitrit Terhadap Potensi Anemia Defisiensi Besi Pada Animal Model Tikus Jantan Wistar

“…Jadi, apabila mencit memiliki berat badan 20 gram maka natrium nitrit yang diberikan adalah sebanyak 0,4 ml/ekor/hari selama 14 hari. Sedangkan untuk tikus jantan (Rattus norvegicus) strain wistar pemberian induksi natrium nitrit (NaNO2) diberikan sebanyak 125 gr/KgBB selama 3 (tiga) minggu (Hermayanti & Syafitri, 2020).…”

“…Kadar hemoglobin pada tikus putih dikatakan normal jika berada pada kisaran 11,6-16,1 g/dL (Douglas dan Wardrop, 2010). Hasil ini sejalan dengan penelitian yang dilakukan oleh Hermayanti, et al (2020) yaitu perlakuan dengan induksi pakan rendah zat besi selama 21 hari belum menunjukkan penurunan kadar hemoglobin pada hewan coba. Secara fisiologis akan terjadi anemia jika cadangan besi yang ada di dalam tubuh tidak cukup untuk digunakan pada proses eritropoesis.…”