Efektivitas Penggunaan Vaksin Dna Dalam Pakan Pada Ikan Mas Yang Diinfeksi Koi Herpesvirus

Abstract

ABSTRAKPenelitian ini bertujuan menentukan frekuensi pemberian vaksin DNA koi herpesvirus (KHV) yang menghasilkan kelangsungan hidup tinggi pada ikan mas yang diinfeksi KHV. Frekuensi pemberian vaksin yaitu vaksinasi satu kali seminggu (K1), vaksinasi dua kali seminggu (K2), vaksinasi tiga kali seminggu (K3), ikan tidak divaksin dan diuji tantang KHV (K4), dan ikan diinjeksi dengan larutan phosphate buffered saline (K5). Pemberian pakan dilakukan 2 kali sehari secara satiasi.Mortalitas pada K1; K2; K3; dan K4 masing-masing adalah 33,33±5,77; 20,00±10,00; 6,67± 5,77; dan 43,33±5,77%, sedangkan kelangsungan hidup relatif (RPS) K1; K2; dan K3 masing-masing adalah 23,07±13,32; 53,84±23,07; 84,60±13,32%. Disimpulkan bahwa pemberian pakan mengandung vaksin DNA anti-KHV dengan frekuensi tiga kali dalam satu minggu efektif meningkatkan kelangsungan hidup ikan mas yang diinfeksi KHV. ____________________________________________________________________________________________________________________ Kata kunci: ikan mas, koi herpesvirus, pakan, vaksin DNA ABSTRACTThis study aims to examine the frequency of koi herpesvirus (KHV) DNA vaccine administration which obtained high survival rate on koi herpesvirus-infected carp. In this study, vaccination was performed by feeding fish with artificial diet containing anti-KHV DNA vaccine. Frequency of vaccine administration tested were vaccination once a week (K1), vaccination twice a week (K2), vaccination three times a week (K3), positive control: the fish were not vaccinated but challenged with KHV (K4), and negative control: fish were injected with phosphate bufferred saline solution (K5). Feeding was performed twice a day at satiation. The mortalily rate on K1, K2, K3, and K4 were 33.33±5.77, 20.00±10.00, 6.67± 5.77, dan 43.33± 5.77 %, respectively. While the survival rate on K1, K2, and K3 were 23.07±13.32, 53.84±23.07, 84.60±13.32 %, respectively. The results showed that vaccination three times a week offered the highest survival rate (84.6 %) and showed higher phagocytic activity compared to the other treatments. Hence, oral vaccination via the feed can effectively increase the survival rate of fish, and this can be as an alternative method to control KHV infection in carp. ____________________________________________________________________________________________________________________

Efficacy of anti-koi herpesvirus DNA vaccine in carp Cyprinus carpio fry by immersion method and hyperosmotic infiltration

“…Based on the results of [12] research, after the fish were challenged with KHV there was a high number of deaths on days 17-18 after being challenged. Likewise in the research of [4], the number of deaths peaked on the 15th to 29th day after the challenge.…”

“…A total of 1 gram of gills was crushed, diluted with sterile PBS to 1 mL, centrifuged at 5000 rpm for 15 minutes at 4-5°C. The supernatant was taken using a micropipette and used as a stock of the virus, for use in fish infection a dilution of 10 -5 was used [4].…”

“…Group A had higher survival (SR) and relative survival (RPS) values than other groups. According to [4], this is because the glycoprotein gene has been inserted into the pAct-GP25 plasmid which is transformed into E. coli DH5α and given to fish is thought to be able to be translated so that glycoproteins from the KHV virus are formed which can then be recognized by the immune system. The fish body responds to the viral protein by forming antibodies and inducing the formation of memory cells so that the presence of memory cells will speed up the formation of secondary responses to the attack of the same antigen.…”

“…An immersion method is an option because giving the vaccine by injection can cause stress to fish. Stressed fish will be easily infected by pathogenic microorganisms [4]. This study aimed to examine the effect of administering a DNA vaccine encoding the glycoprotein KHV virus on carp in the seed stage through immersion and hyperosmotic infiltration to prevent infection with the KHV virus in carp fry [1,5].…”