Background: With the emergence of functional movement in the study of athleticism, Pilates exercise based on the activation of the body core is attracting much attention. Objective: This study aimed to investigate the changes in functional movement after performing Pilates exercise. Methods: A total of 31 college students at university K in seoul were included in this study. These students performed a 90-minute Pilates exercise twice a week for 16 weeks. Frequency analysis and paired t-test were performed using IBM SPSS ver. 25.0 (IBM Corp., Armonk, NY, USA). Results: All the seven factors of functional movement screen (FMS) showed statistically significant results. Conclusion: Considering that the levels of all the seven factors of FMS improved after performing Pilates exercise, Pilates exercise is considered superior in improving functional movement.