Effect of 17β-estradiol on the in vitro maturation of bovine oocytes

Beker, A.; Colenbrander, B.; Bevers, M.M.

doi:10.1016/s0093-691x(02)01082-8

Cited by 56 publications

(50 citation statements)

References 22 publications

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“…It has been reported that the presence of 17β-estradiol (E 2 ) in maturation medium inhibits spontaneous germinal vesicle breakdown (GVBD) an d/or maturation to metaphase-II (M-II) in pig [6,7], mouse [8] and cattle [9] oocytes. In contrast, negative effects of E 2 on male pronuclear (MPN) formation have also been reported in pig oocytes [10].…”

mentioning

confidence: 99%

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Niwa

Hunter

2004

Journal of Reproduction and Development

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Abstract. The present study examined the effects of 17β-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P<0.05), and cumulus expansion was also significantly inhibited (P<0.05) compared with the control (no E2 added). Although oocytes matured in the presence of E2 were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P<0.05) than the control. These inhibitory effects of E2 were prevented when the medium was supplemented with E2 together with its antagonist, ICI 182,780 (1 µg/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E2 had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E2. When cumulus-enclosed oocytes were cultured in the presence of progesterone (P4; 600 ng/ml) alone or together with E2, no significant differences in nuclear maturation, cumulus expansion or penetration in vitro were observed compared with control oocytes. The concentration of P4 in maturation medium was significantly (P<0.01) lower when cumulus-enclosed oocytes were cultured for 44 h in the medium with E2 than in medium without E2. These results indicate that E2 inhibits both nuclear and cytoplasmic maturation of cumulus-enclosed pig oocytes, and that this inhibition can be prevented by an E2 antagonist or P4. This E2 inhibition may occur indirectly via the cumulus cells and inhibition of P4 synthesis. Key words: 17β-estradiol, In vitro fertilization, In vitro maturation, Oocyte, Pig (J. Reprod. Dev. 50: [305][306][307][308][309][310][311][312][313] 2004) n mammalian follicles, oocytes are surrounded by follicular cells in antral follicles and grow and mature in an environment of follicular fluid. During follicular development, the concentration of estrogen in follicular fluid gradually increases due to stimulation by follicle stimulating hormone (FSH) [1][2][3][4][5]. However, after the ovulatory lutenizing hormone (LH) surge or treatment with human chorionic gonadotrophin (hCG), the concentration of estrogen decreases drastically, and an increase in the concentration of progesterone follows, shortly before ovulation [1][2][3][4][5]. In addition to these changes in the concentrations of estrogen and progesterone in follicular fluid, the LH surge also stimulates meiotic resumption in oocytes. Therefore, it is possible that these steroids play an important role(s) not only for nuclear maturation

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confidence: 99%

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Niwa

Hunter

2004

Journal of Reproduction and Development

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“…In many studies, supplementation of 1000 ng mL −1 E 2 to the IVM medium was effective for resumption of meiosis and promotion of maturation of bovine oocytes (Fukui et al, 1982;Fukushima and Fukui, 1985;Younis et al, 1989;Beker et al, 2002). P 4 was added to the IVM medium for bovine oocytes at a concentration of 50-5000 ng mL −1 , and various results were reported on embryo production (Silva and Knight, 2000;Aparicio et al, 2011;Syoji et al, 2014).…”

Section: Matsuo Et Al: Three-step In Vitro Maturation Culture Of mentioning

confidence: 99%

“…Temperature (Lenz et al, 1983), oxygen concentration (Hashimoto et al, 2000), nutrients (Takahashi and First, 1992;Kim et al, 1993) and hormones (Fukui et al, 1982;Silva and Knight, 2000;Beker et al, 2002;Mingoti et al, 2002) during in vitro maturation (IVM) culture are considered as factors to improve in vitro development of bovine oocytes. However, developmental rate of bovine oocytes to blastocyst in vitro was still lower than that of in vivo (Leibfried-Rutledge et al, 1987;Rizos et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17<i>β</i> and progesterone concentrations in preovulatory follicular fluid

Matsuo¹,

Sumitomo²,

Ogino³

et al. 2017

Arch. Anim. Breed.

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Abstract. The objective of the article is to evaluate the effect of three-step in vitro maturation (IVM) culture system imitating estradiol-17β (E 2 ) and progesterone (P 4 ) concentrations in preovulatory follicles on in vitro bovine embryo production. The cumulus-oocyte complexes (COCs) were collected from follicles (2 to 8 mm in diameter) of bovine ovaries obtained from a local slaughterhouse. For IVM, the COCs were cultured for 22 h in a three-step system: (1) culture in medium 199, containing 700 ng mL −1 E 2 and 50 ng mL −1 P 4 , for 5 h, followed by the medium containing 150 ng mL −1 E 2 and 150 ng mL −1 P 4 for 11 h, and then the medium containing 20 ng mL −1 E 2 and 300 ng mL −1 P 4 for 6 h (EP group); (2) culture in the medium containing 700 ng mL −1 E 2 for 5 h, followed by the medium containing 150 ng mL −1 E 2 for 11 h, and then the medium containing 20 ng mL −1 E 2 for 6 h (E group); or (3) culture in the medium containing 50 ng mL −1 P 4 for 5 h, followed by the medium containing 150 ng mL −1 P 4 for 11 h, and then the medium containing 300 ng mL −1 P 4 for 6 h (P group). The COCs were cultured in the medium containing 1000 ng mL −1 E 2 for 22 h (control group). After IVM, the COCs were co-incubated with sperm and further cultured. At 48 h after insemination, the cleavage rate of embryos was not different among the groups. At 192 h after insemination, the blastocyst formation rate of EP group was significantly higher than that of the other groups. The total cell number of blastocysts did not differ among the groups. In conclusion, these results demonstrate that the three-step IVM culture system of bovine oocytes imitating temporal changes of E 2 and P 4 concentrations in preovulatory follicular fluid improves the developmental potential of embryos in vitro.

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“…While we speculated that altered FSHR function was the likely cause of seasonal fluctuations in oocyte FSH sensitivity, we also predicted that it would be an oversight to exclude a pathway known to synergize with FSH. Therefore, we also measured estrogen receptor b (ESR2), a receptor coactivated by FSH in granulosa cells (Kouzu-Fujita et al 2009) and which likely plays a role in directly regulating estrogen actions (Pasapera et al 2005), especially meiotic resumption (bovine, Beker et al 2002, Beker-van Woudenberg et al 2004canine, Kim et al 2005;cervidae, Siriaroonrat et al 2010;porcine, Kim et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

Hobbs

Howard²,

Wildt³

et al. 2012

Reproduction

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Domestic cat oocytes are seasonally sensitive to FSH. Compared with those collected during the breeding season, oocytes from the nonbreeding (NB) season require more FSH during in vitro maturation to achieve comparable developmental competence. This study tested the hypothesis that this seasonal variation was due to altered expression of FSH receptors (FSHR) and/or FSH-induced genes. Relative expression levels of FSHR mRNA and FSH-enhanced gene estrogen receptor b (ESR2) were measured by qPCR in whole ovaries and immature cumulus-oocyte complexes (COCs) isolated from cat ovaries during the natural breeding vs NB seasons. Expression levels of FSH-induced genes prostaglandin-endoperoxide synthase 2 (PTGS2), early growth response protein-1 (EGR1), and epidermal growth factor receptor (EGFR) were examined in mature COCs from both seasons that were a) recovered in vivo or b) matured in vitro with conventional (1 mg/ml) or high (10 mg/ml) FSH concentrations. Overall, FSHR mRNA levels were lower in whole ovaries during the NB compared with breeding season but were similar in immature COCs, whereas ESR2 levels did not differ in either group between intervals. We observed changes in PTGS2, EGR1, and EGFR mRNA expression patterns across maturation in COCs within but not between the two seasons. The lack of seasonal differentiation in FSH-related genes was not consistent with the decreased developmental capacity of oocytes fertilized during the NB season. These findings reveal that the seasonal decrease in cat oocyte sensitivity to FSH occurs both in vivo and in vitro. Furthermore, this decline is unrelated to changes in expression of FSHR mRNA or mRNA of FSH-induced genes in COCs from antral follicles.

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Effect of 17β-estradiol on the in vitro maturation of bovine oocytes

Cited by 56 publications

References 22 publications

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17<i>β</i> and progesterone concentrations in preovulatory follicular fluid

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

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Effect of 17β-estradiol on the in vitro maturation of bovine oocytes

Cited by 56 publications

References 22 publications

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Effects of 17.BETA.-Estradiol on In Vitro Maturation of Pig Oocytes in Protein-Free Medium

Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17&lt;i&gt;β&lt;/i&gt; and progesterone concentrations in preovulatory follicular fluid

Absence of seasonal changes in FSHR gene expression in the cat cumulus–oocyte complex in vivo and in vitro

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Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17<i>β</i> and progesterone concentrations in preovulatory follicular fluid