Effect of 2,4-dichlorophenoxyacetic acid herbicide onEscherichia coli growth, chemical composition, and cellular envelope

Balagué, Claudia; Stürtz, Nelson; Duffard, Ricardo; Duffard, Ana María Evangelista de

doi:10.1002/1522-7278(2001)16:1<43::aid-tox50>3.0.co;2-r

Cited by 27 publications

(10 citation statements)

References 37 publications

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“…Balagué et al [53] used doses of 2 mM, 1 mM, 0.1 mM and 0.01 mM of the herbicide 2,4-D in spectrophotometer analysis, and verified growing inhibition of E. coli HB101 only at 2 mM, whilst in the present study, the growth capacity, analyzed by cellular viability, showed significant differences in E. coli DH5-α between control and mesotrione treatments in 30 min. evaluation (Fig.…”

Section: Resultssupporting

confidence: 70%

“…These changes in membrane lipids occur from chloroacetanilide herbicides. A study by Balangué et al [53] with E. coli HB101 strain demonstrated that the toxicity of 2,4-D could reduce the fluidity of bacterial membrane, and consequently alter MDA results, as a system-wide response against ROS. Sánchez et al [56] also reported similar findings, where the strain Klebsiella planticola DSZ, when in contact with ethanol or the herbicide simazine, exhibited a decrease in the saturation of membrane lipids, altering the rate of selective permeability, possibly as a defense system.…”

Section: Resultsmentioning

confidence: 99%

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Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

et al. 2014

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The intensive use of agrochemicals has played an important role in increasing agricultural production. One of the impacts of agrochemical use has been changes in population structure of soil microbiota. The aim of this work was to analyze the adaptive strategies that bacteria use to overcome oxidative stress caused by mesotrione, which inhibits 4-hydroxyphenylpyruvate dioxygenase. We also examined antioxidative stress systems, saturation changes of lipid membranes, and the capacity of bacteria to degrade mesotrione. Escherichia coli DH5-á was chosen as a non-environmental strain, which is already a model bacterium for studying metabolism and adaptation. The results showed that this bacterium was able to tolerate high doses of the herbicide (10× field rate), and completely degraded mesotrione after 3 h of exposure, as determined by a High Performance Liquid Chromatography. Growth rates in the presence of mesotrione were lower than in the control, prior to the period of degradation, showing toxic effects of this herbicide on bacterial cells. Changes in the saturation of the membrane lipids reduced the damage caused by reactive oxygen species and possibly hindered the entry of xenobiotics in the cell, while activating glutathione-S-transferase enzyme in the antioxidant system and in the metabolizing process of the herbicide. Considering that E. coli DH5-α is a non-environmental strain and it had no previous contact with mesotrione, the defense system found in this strain could be considered non-specific. This bacterium system response may be a general adaptation mechanism by which bacterial strains resist to damage from the presence of herbicides in agricultural soils.

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Section: Resultssupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

et al. 2014

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“…Changes in the conformation of saturated and unsaturated fatty acids of the plasma membrane could be considered a protective mechanism by bacteria in contact with herbicides (Murínová and Dercová 2014 ; Segura et al 1999 ). Balague et al ( 2001 ) reported a decrease in unsaturated lipids in the plasma membrane of E. coli HB101 after treatment with the herbicide 2,4-D, and these authors considered the reduction in membrane fluidity as a possible defense mechanism against cell damage. Moreover, Sánchez et al ( 2005 ) reported that an increase in the saturation level of membrane lipids in Klebsiella planticola DSZ allowed growth in a culture medium containing the herbicide simazine.…”

Section: Discussionmentioning

confidence: 99%

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

et al. 2016

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Callisto®, containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. The objective of this work was to evaluate enzymatic and structural changes in Pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. Despite degradation of mesotrione, probably due a glutathione-S-transferase (GST) pathway in Pantoea ananatis, this herbicide induced oxidative stress by increasing hydrogen peroxide production. Thiol fragments, eventually produced after mesotrione degradation, could be involved in increased GST activity. Nevertheless, there was no peroxidation damage related to this production, as malondialdehyde (MDA) synthesis, which is due to lipid peroxidation, was highest in the controls, followed by the mesotrione- and Callisto®-treated cultures at log growth phase. Therefore, P. ananatis can tolerate and grow in the presence of the herbicide, probably due an efficient control of oxidative stress by a polymorphic catalase system. MDA rates depend on lipid saturation due to a pattern change to a higher level of saturation. These changes are likely related to the formation of GST-mesotrione conjugates and mesotrione degradation-specific metabolites and to the presence of cytotoxic adjuvants. These features may shift lipid membrane saturation, possibly providing a protective effect to bacteria through an increase in membrane impermeability. This response system in P. ananatis provides a novel model for bacterial herbicide tolerance and adaptation in the environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0240-x) contains supplementary material, which is available to authorized users.

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“…Changes in membrane fluidity are a primary effect observed in herbicide-tolerant bacteria and are caused by lipid peroxidation [ 12 ]. This adjustment in the composition and structure of membrane lipids can reduce the damage caused by ROS, decrease the energy expenditure, limit the entry of xenobiotics into the cell and optimize cell growth [ 5 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

Dobrzanski¹,

Gravina²,

Steckling³

et al. 2018

PLoS ONE

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The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

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Effect of 2,4-dichlorophenoxyacetic acid herbicide onEscherichia coli growth, chemical composition, and cellular envelope

Cited by 27 publications

References 37 publications

Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

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