Effect of 2,4-Dichlorophenoxyacetic Acid on the Efficiency of Wheat Haploid Production by the<i>Hordeum bulbosum</i>Method

Ushiyama, Tomohiko; Kuwabara, Tatsuo; Yoshida, Tomohiko

doi:10.1626/pps.9.206

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…The embryo development was inhibited by the treatment with over 50 mg L -1 2,4-D. In the crosses of wheat x H. bulbosum, similar results was observed by Ushiyama et al (2006), who pointed out that the treatment with 2,4-D at the concentration of 100 mg L -1 or less was effective for haploid wheat production while it reduced embryos sizes and arrested embryo development into plant. However, the mechanism of the effect of 2,4-D was not elucidated .…”

Section: Discussionsupporting

confidence: 76%

“…Marshall et al (1983) reported that the application of a single drop of 80 mg L -1 or 160 mg L -1 2,4-D solution to them increased the size of seeds, and Inagaki (1986) reported that 70.2% of the unpollinated florets set seeds without embryos when the culms were injected with 1000 ppm 2,4-D. Falk and Kasha (1982) reported that the unpollinated spikes sprayed with 0.1 and 1.0 mg L -1 IAA did not set seeds. Ushiyama et al (2006) investigated the influence of various concentrations of 2,4-D on the production with wheat haploid and pointed out that the treatment with 2,4-D at 125 to 175 mg L -1 increased the percentage of the seed setting, but reduced the formation and development of the embryo. Therefore, it appears that the promoting effect of 2,4-D and NAA on seed setting is not reflected in fertilization and embryo development.…”

Section: Discussionmentioning

confidence: 99%

“…The maize seedlings were grown in a greenhouse kept at the minimum temperature of 20ºC. For haploid wheat cultivation, we used intergeneric crossing and embryo culture followed by the bulbosum system of Ushiyama et al (2006). The experiment to determine useful phytohormones was carried out in Controls indicated that detached spikes were cultured in the medium without phytohormone.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses

Ushiyama

Kuwabara

Yoshida

2007

Plant Production Science

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses

Ushiyama

Kuwabara

Yoshida

2007

Plant Production Science

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“…The same observation was reported by García-Llamas, Martín & Ballesteros (2004) which showed that treatment with 100 mg/L 2,4-D significantly increased the production of embryos and haploid plants and this concentration of 2,4-D was optimal for bread wheat × maize cross. Ushiyama & Kuwabara (2006) suggested that treatment with 2,4-D at 100 mg/L was also effective for haploid wheat production by Hordeum bulbosum method. 2,4-D in concentration 125, 150, 175 mg/L was used by Sourour, Olfa & Hajer (2011) and in 1000 mg/L by Suenaga (1994) .…”

Section: Discussionmentioning

confidence: 99%

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Juzoń

Warchoł

Dziurka

et al. 2022

PeerJ

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Background Development of new cultivars is one of the vital options for adapting agriculture to climate change, and the production of doubled haploid (DH) plants can make a significant contribution to accelerating the breeding process. Oat is one of the cereals with particular health benefits, but it unfortunately still remains recalcitrant to haploidization. Our previous studies have clearly demonstrated that post-pollination with hormone treatment is a key step in haploid production through wide hybridization and indicated it as the most effective method for this species. Therefore, we subsequently addressed the problem of the influence of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on consecutive stages of DH production. Methods Twenty-nine genotypes were tested, 9,465 florets were pollinated with maize pollen 2 days after emasculation and then treated with 2,4-D at 50 mg/L and 100 mg/L. Results The applied treatments did not reveal any differences in the number of obtained haploid embryos. However, almost twice as many haploid plants formed on MS medium after applying a higher auxin concentration and 20% more successfully acclimatized. Moreover, 100 mg/L 2,4-D treatment resulted in twice as many DH lines that produced almost three times more seeds compared to 50 mg/L treatment. Nevertheless, the results have confirmed the existence of strong genotypic variation, which may significantly limit the development of an effective and economically feasible method that could be incorporated into breeding programs.

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“…In addition to the haploid breeding method (Ushiyama et al, 2006), gathering a lot of major genes with high yield and high quality by conventional cross breeding method is necessary. Pedigree trees of modern cultivars are very complicated and it is almost impossible to evaluate the performance and the combining ability of the cultivar from the pedigree tree.…”

Section: The Relationship Between the Performance Of Cultivars And Thmentioning

confidence: 99%

Pedigree Analysis of Early Maturing Wheat Cultivars in Japan for Breeding Cultivars with Higher Performance

Ushiyama

Nakamura

Yoshida

2009

Plant Production Science

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: Pedigree analysis was conducted for early maturing wheat cultivars developed in Japan. Materials used for this analysis were mainly developed at Nagano Agricultural Experiment Station (Tozan lines). In a recently released Tozan line, the maximum number of generations traced in the pedigree, total number of ancestors in the pedigree and total number of ancestors except common ones was 11, 222 and 94, respectively. Chunaga contributed 24.0% of the genetic background of Tozan lines. Seven ancestors, collectively, contributed 51.5% to the gene pool. Hiyokukomugi had the highest mean coeffi cient of parentage to Tozan lines and the mean value was 0.216, followed by Kinuiroha (0.213), Norin 61 (0.206), Mikunikomugi (0.205) and Tokai 80 (0.194). The mean coeffi cient of parentage between Tozan lines and cultivars in the Kanto-Tokai region, Kinki-ChugokuShikoku region and Kyushu region was 0.165, 0.155 and 0.157, respectively. Tozan lines more related to cultivars in the Tohoku region tended to be late heading and more cold-tolerant. Tozan lines more related to Ayahikari or Kinuazuma tended to be early maturing. Fukuhokomugi, which was a high yield cultivar and often used as a cross parent, did not contribute to high fl our protein. KS831957 showed a positive effect on the crude protein content of flour. In general, Tozan lines had no specific cultivars used extensively as a cross parent having significant infl uence on yield or fl our quality.

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Effect of 2,4-Dichlorophenoxyacetic Acid on the Efficiency of Wheat Haploid Production by theHordeum bulbosumMethod

Cited by 3 publications

References 12 publications

Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses

Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Pedigree Analysis of Early Maturing Wheat Cultivars in Japan for Breeding Cultivars with Higher Performance

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