Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions

Moon, Eun‐Kyung; Lee, Seung‐Eun; Quan, Fu‐Shi; Kong, Hyun-Hee

doi:10.3347/kjp.2018.56.5.491

Cited by 4 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the biocides used in the OPTI-FREE products, namely, polyquaternium-1 and myristamidopropyl dimethylamine, have been shown to have the greatest efficacy against Acanthamoeba (and other ophthalmological pathogens) as compared with other biocides. [34][35][36] The results of the current investigation indicate similar results: products containing polyquaternium-1 and myristamidopropyl dimethylamine demonstrated significantly greater Acanthamoeba trophozoite disinfection efficacy than other biocides or combinations of biocides. According to the results demonstrated here, less effective biocides include polyaminopropyl biguanide or alexidine dihydrochloride combined with polyquaternium or polyquaternium-1.…”

Section: Wwwoptvisscicomsupporting

confidence: 71%

“…OPTI-FREE products have also previously been found to produce little or no encystment, 32,33 ensuring that the more vulnerable trophozoite form persists and is able to be acted upon by multipurpose solutions. Finally, the biocides used in the OPTI-FREE products, namely, polyquaternium-1 and myristamidopropyl dimethylamine, have been shown to have the greatest efficacy against Acanthamoeba (and other ophthalmological pathogens) as compared with other biocides 34–36 …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Antimicrobial Efficacy of Multipurpose Solutions against Acanthamoeba Trophozoites

et al. 2021

View full text Add to dashboard Cite

SIGNIFICANCE This investigation examines the effectiveness of several common contact lens solutions in the disinfection of Acanthamoeba , which causes a serious eye infection most often resulting from dysfunctional or improper use of contact lens products. PURPOSE Acanthamoeba keratitis is an eye infection caused by a free-living amoeba, which can lead to extensive corneal damage and frequently blindness. Acanthamoeba keratitis is linked with contact lens use combined with noncompliance with contact lens care cleaning regimens. The patient's choice and use of multipurpose solutions (MPSs) continue to be a risk factor for Acanthamoeba keratitis. Thus, it is critical that the Acanthamoeba disinfection efficacy of the popular MPSs be determined. Here we compare the efficacy of seven major MPSs on the global market. METHODS Using standard methods of Acanthamoeba disinfection and quantification, Acanthamoeba ATCC 30461, 30868, 50370, and 50676 trophozoites were inoculated into each MPS and held for the manufacturer's recommended disinfection time. Acanthamoeba recovery plates were incubated for 14 days, after which positive wells were identified and cell concentrations determined using the 50% endpoint method. RESULTS Members of the OPTI-FREE products (Express, Replenish, and Puremoist [Alcon, Fort Worth, TX]) demonstrated significantly higher percentages of antimicrobial activity compared with the renu Advanced Formula (Bausch + Lomb, Rochester, NY), Biotrue (Bausch + Lomb), Acuvue RevitaLens (Johnson & Johnson, Santa Ana, CA), and Lite products (Cooper Vision, Scottsville, NY) for four of the trophozoite strains tested. CONCLUSIONS Many of the popular MPS biocides maintain little or no antimicrobial activity against Acanthamoeba trophozoites, and the number of biocides in an MPS does not necessarily indicate its antimicrobial activity.

show abstract

Section: Wwwoptvisscicomsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Differential Antimicrobial Efficacy of Multipurpose Solutions against Acanthamoeba Trophozoites

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The structure of the cyst contains a cellulose double wall with a fibrous laminar exocyst and a granular smooth endocyst. The composition of the cyst wall contains 1,4 b-glucan, also known as cellulose, 37 and other polysaccharides that make the wall more robust for protection. 38 After decades of storage, cysts can remain pathogenic and viable.…”

Section: Life Cycle Of Acanthamoebamentioning

confidence: 99%

Acanthamoeba keratitis: a review of biology, pathophysiology and epidemiology

Lacerda

Lira

2020

Ophthalmic Physiologic Optic

View full text Add to dashboard Cite

Acanthamoeba keratitis is an infection caused by a unicellular protozoan of the genus Acanthamoeba that is universally widespread. Until now, most cases were reported in contact lens wearers, although it is also a reality for non‐wearers, mostly connected to corneal trauma. There is also a variation in incidence regarding the aetiology of the disease between developed and developing countries. Purpose This work is based on a literature review, and the main goal is to deepen the knowledge about Acanthamoeba keratitis, presenting the main risk factors and focusing on prevention actions for this type of corneal infection since the treatments are not always effective. It targets specialists in visual health to strengthen their knowledge in this area, as well as to allow them to better inform their patients about hygiene care, appropriate measures of disinfection and ways to minimise the risk of infection. At this stage, it is important to highlight the essential role that practitioners play in fitting, monitoring and following‐up patients to minimise the danger of infection. Recent findings It is well recognised that corneal trauma facilitates invasion by leaving an open door for microorganisms to penetrate the cornea. In addition to trauma, risk factors are mostly associated with patients’ behaviours, such as interaction of contact lenses with contaminated water in the shower, swimming pools and beaches, etc., lack of hygiene habits with contact lenses and respective cases, and the use of ineffective disinfecting solutions. The fact that a disinfecting solution is not completely effective against trophozoites and/or cysts, both forms of Acanthamoeba’s lifecycle, can cause the infection since one cyst alone leads to the emergence of a whole new population of Acanthamoeba. Summary It is necessary to reduce the risk of infection and, beyond the need to promote patient education to encourage correct CL hygiene behaviours, it should also be highlighted that there is an urgent need to enhance the efficacy of CL disinfection systems against all strains and both stages of Acanthamoeba through the creation of standardised methods. The ease of purchasing CLs without any supervision must also be considered a concern, and, in the near future, it is also important to develop and implement effective diagnostic methods and treatments for Acanthamoeba keratitis.

show abstract

“…They exert their effects by suppressing phospholipid biosynthesis, disruption of cellular membranes and intracellular signalling, and induction of apoptosis [30]. The herbicide 2,6-dichlorobenzonitrile inhibits cellulose synthesis and encystment of the amoeba [31]. The disinfectant povidone-iodine (PVP-I) has both trophicidal and cysticidal effects in vitro [32].…”

Section: Current Anti-acanthamoebic Therapymentioning

confidence: 99%

“…It works by damaging protein and nucleic acid synthesis [33]. In vitro study showed that at 0.5-2.5% PVP-I has a better activity on the trophozoite and cystic stages than CHX [31]. In vitro screening of derivatives of the heterocyclic compounds 1H-benzimidazole and 1H-benzotriazole, and their N-alkylated analogues against A. castellanii identified 5,6-dimethyl-1H-benzotriazole and 5,6-dibromo-1H-benzotriazole to be more cysticidal compared to CHX [34].…”

Section: Current Anti-acanthamoebic Therapymentioning

confidence: 99%

Drug Discovery against Acanthamoeba Infections: Present Knowledge and Unmet Needs

2020

View full text Add to dashboard Cite

Although major strides have been made in developing and testing various anti-acanthamoebic drugs, recurrent infections, inadequate treatment outcomes, health complications, and side effects associated with the use of currently available drugs necessitate the development of more effective and safe therapeutic regimens. For any new anti-acanthamoebic drugs to be more effective, they must have either superior potency and safety or at least comparable potency and an improved safety profile compared to the existing drugs. The development of the so-called ‘next-generation’ anti-acanthamoebic agents to address this challenge is an active area of research. Here, we review the current status of anti-acanthamoebic drugs and discuss recent progress in identifying novel pharmacological targets and new approaches, such as drug repurposing, development of small interfering RNA (siRNA)-based therapies and testing natural products and their derivatives. Some of the discussed approaches have the potential to change the therapeutic landscape of Acanthamoeba infections.

show abstract

Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions

Cited by 4 publications

References 13 publications

Differential Antimicrobial Efficacy of Multipurpose Solutions against Acanthamoeba Trophozoites

Differential Antimicrobial Efficacy of Multipurpose Solutions against Acanthamoeba Trophozoites

Acanthamoeba keratitis: a review of biology, pathophysiology and epidemiology

Drug Discovery against Acanthamoeba Infections: Present Knowledge and Unmet Needs

Contact Info

Product

Resources

About