Effect of 3 lactobacilli on immunoregulation and intestinal microbiota in a β-lactoglobulin–induced allergic mouse model

Fu, Guiming; Zhao, Kui; Chen, Hui; Wang, Yuanyuan; Nie, Lijuan; Wei, Hua; Wan, Cuixiang

doi:10.3168/jds.2018-15683

Cited by 62 publications

(41 citation statements)

References 68 publications

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“…However, we speculate that several modifications of the microbiota induced by LA305 could reinforce this effect. Ralstonia spp., known for their proinflammatory effects, were depleted, as previously observed by Fu et al following Lactobacillus treatment in another CMA mouse model (33). Similarly, Clostridium sensu stricto, a genus that has been associated with the microbiota of allergic children harboring an IgE-dependent food allergy or atopic dermatitis (34,35), was also decreased.…”

Section: Discussionsupporting

confidence: 63%

Three Candidate Probiotic Strains Impact Gut Microbiota and Induce Anergy in Mice with Cow's Milk Allergy

Esber

Mauras

Delannoy

et al. 2020

Appl Environ Microbiol

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Cow's milk allergy is a worldwide public health issue, especially since there is no effective treatment, apart from milk and dairy product avoidance. The aim of this study was to assess the beneficial role of three probiotic strains previously selected for their prophylactic properties in a mouse model of β-lactoglobulin allergy. Administration of Lactobacillus rhamnosus LA305, L. salivarius LA307, or Bifidobacterium longum subsp. infantis LA308, for 3 weeks post-sensitization and challenge modified the composition of the gut microbiota with an increase in Prevotella NK3B31 and a decrease in Marvinbryantia, belonging to the Lachnospiraceae group. Although no impact on markers of sensitization was detected, modifications of foxp3, tgfβ and il10 ileal gene expression as well as plasma metobolomic alteration in the tryptophan pathway were observed. Moreover, ex-vivo studies showed that all probiotic strains induced a significant decrease in cytokine production by β-lactoglobulin-stimulated splenocytes. Altogether, these results suggest that the three probiotic strains tested lead to alterations in immune responses i.e. induction of a tolerogenic anergy and anti-inflammatory responses. This anergy could be linked to cecal microbiota modifications although no impact on fecal SCFA concentration was detected. Anergy could also be linked to a direct impact of probiotic strains on dendritic cells as co-stimulatory molecule expression was decreased following their co-incubation with BMDCs. To conclude, all three candidate probiotic strains induced strain-specific gut microbiota and metabolic changes, which could potentially be beneficial for general health, as well as anergy that could contribute to oral tolerance acquisition. IMPORTANCE We previously showed that three probiotic strains i.e. Lactobacillus rhamnosus LA305, L. salivarius LA307, and Bifidobacterium longum subsp. infantis LA308 exerted different preventive effects in a mouse model of cow's milk allergy. In this study, we evaluated their potential benefits in a curative mouse model of cow's milk allergy. When administered for 3-weeks after the sensitization process and a first allergic reaction, none of the strains modified the levels of sensitization and allergic markers. However, all three strains affected gut bacteria communities and modified immune and inflammatory responses leading to a tolerogenic profile. Interestingly, all three strains exerted a direct effect on dendritic cells which are known to play a major role in food sensitization through their potentially tolerogenic properties and anergic responses. Altogether, these data indicate a potentially beneficial role of the probiotic strains tested in this model of cow's milk allergy with regard to tolerance acquisition.

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Section: Discussionsupporting

confidence: 63%

Three Candidate Probiotic Strains Impact Gut Microbiota and Induce Anergy in Mice with Cow's Milk Allergy

Esber

Mauras

Delannoy

et al. 2020

Appl Environ Microbiol

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“…B. animalis subspecies lactis Bb12 treatment improved expression of genes related to Th1/Th2 cells, inflammatory cells, Treg, and physiological function in the gut and reduced Th2 type immune responses. In blactoglobulin-induced allergic mice, L. plantarum ZDY2013, L. plantarum WLPL04 and L. rhamnosus GG increased Th1 cells differentiation and inhibited the Th2-biased immune response (113). Furthermore, Treg differentiation not only regulates Th1/Th2 immune balance but suppresses Th17-biased response.…”

Section: Regulation Of Probiotics On Immune Responses In Admentioning

confidence: 95%

Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review

et al. 2021

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Atopic dermatitis (AD) is a public health concern and is increasing in prevalence in urban areas. Recent advances in sequencing technology have demonstrated that the development of AD not only associate with the skin microbiome but gut microbiota. Gut microbiota plays an important role in allergic diseases including AD. The hypothesis of the “gut-skin” axis has been proposed and the cross-talk mechanism between them has been gradually demonstrated in the research. Probiotics contribute to the improvement of the intestinal environment, the balance of immune responses, regulation of metabolic activity. Most studies suggest that probiotic supplements may be an alternative for the prevention and treatment of AD. This study aimed to discuss the effects of probiotics on the clinical manifestation of AD based on gut microbial alterations. Here we reviewed the gut microbial alteration in patients with AD, the association between gut microbiota, epidermal barrier, and toll-like receptors, and the interaction of probiotics and gut microbiota. The potential mechanisms of probiotics on alleviating AD via upregulation of epidermal barrier and regulation of immune signaling had been discussed, and their possible effective substances on AD had been explored. This provides the supports for targeting gut microbiota to attenuate AD.

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“…In their hands only the lower allergen dose (5 mg extract), but not the higher dose (25 mg extract) was able to successfully induce allergic sensitization [40]. Moreover, Li et al defined a symptom score for the evaluation of the induced allergic reactions (Table 2) which has proven very useful and thus has been frequently used for other food allergy models [17,49,50,69,70,71].…”

Section: Types Of Mouse Food Allergy Modelsmentioning

confidence: 99%

Mouse Models for Food Allergies: Where Do We Stand?

Schülke

Albrecht

2019

Cells

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Food allergies are a steadily increasing health and economic problem. Immunologically, food allergic reactions are caused by pathological, allergen-specific Th2 responses resulting in IgE-mediated mast cell degranulation and associated inflammatory reactions. Clinically, food allergies are characterized by local inflammation of the mouth mucosa, the face, the throat, the gastrointestinal tract, are frequently paralleled by skin reactions, and can result in life-threatening anaphylactic reactions. To better understand food allergies and establish novel treatment options, mouse models are indispensable. This review discusses the available mouse food allergy models, dividing them into four categories: (1) adjuvant-free mouse models, (2) mouse models relying on adjuvants to establish allergen-specific Th2 responses, (3) mouse models using genetically-modified mouse strains to allow for easier sensitization, and (4) humanized mouse models in which different immunodeficient mouse strains are reconstituted with human immune or stem cells to investigate humanized immune responses. While most of the available mouse models can reproducibly portray the immunological parameters of food allergy (Th2 immune responses, IgE production and mast cell activation/expansion), so far, the recreation of the clinical parameters has proven more difficult. Therefore, up to now none of the available mouse models can reproduce the complete human pathology.

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Effect of 3 lactobacilli on immunoregulation and intestinal microbiota in a β-lactoglobulin–induced allergic mouse model

Cited by 62 publications

References 68 publications

Three Candidate Probiotic Strains Impact Gut Microbiota and Induce Anergy in Mice with Cow's Milk Allergy

Three Candidate Probiotic Strains Impact Gut Microbiota and Induce Anergy in Mice with Cow's Milk Allergy

Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review

Mouse Models for Food Allergies: Where Do We Stand?

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