Effect of a fibrolytic enzyme preparation from <i>Trichoderma longibrachiatum</i> on the rumen microbial population of dairy cows

Nsereko, V. L.; Beauchemin, K. A.; Morgavi, Diego P.; Rode, L. M.; Furtado, Alister; McAllister, Tim A.; Iwaasa, A. D.; Yang, Wenzhu; Wang, Y.

doi:10.1139/w01-131

Cited by 118 publications

(125 citation statements)

References 18 publications

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“…Nsereko et al (6) suggested that exogenous enzymes could increase fiber degradation through a hydrolytic action resulting in more effective rumen fermentation. Tang et al (30) suggested that in vitro GP variables usually reflect the characteristics of the fermentation process.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 180 bottles (three bottles for each ration in addition to three bottles for each enzyme in three different runs with three bottles as blanks (rumen fluid only)) were incubated for 72 h. The technique of Theodorou et al (16) was used for measuring the pressure of gas produced at 2, 4,6,8,10,12,24,36,48, and 72 h of incubation employing a pressure transducer to measure gas from incubations in 120-mL gas-tight culture bottles. Gas accumulated in the headspace of the bottle as the fermentation proceeded was measured with a digital manometer (Extech Instruments, Waltham, MA, USA).…”

Section: In Vitro Incubationsmentioning

confidence: 99%

“…Exogenous enzymes treatments affect the forage fiber structure, which could stimulate microbial colonization (3). Exogenous fibrolytic enzymes also accelerate the rate of digestion by enhancing attachment and/or improve access of rumen microorganisms to the cell wall matrix (6).…”

Section: Introductionmentioning

confidence: 99%

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Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Elghandour¹,

Kholif²,

Molina³

et al. 2015

Turk J Vet Anim Sci

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This study assessed the effects of cellulase, xylanase, and their mixture (1:1, v/v) on in vitro rumen fermentation of five mixed rations with different maize silage (F) to concentrate (C) ratios (0F:100C, 25F:75C, 50F:50C, 75F:25C, 100F:0C). Samples were incubated using rumen inoculum from Brown Swiss cows fed ad libitum a total mixed ration of concentrate and alfalfa hay (1:1). Gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h of incubation. Interaction effects (P < 0.0001) were observed between type of ration and enzyme for discrete lag time prior to GP. Ration type affected asymptotic GP (linear and quadratic, P < 0.0001) and in vitro GP at 24 h onward. Moreover, rations affected pH (linear and quadratic, P < 0.0001), dry matter degradability (quadratic, P = 0.05), metabolizable energy (linear, P = 0.038), short-chain fatty acids (linear, P = 0.0005), gas yield from truly digested dry matter at 24 h (linear, P = 0.0026), and microbial crude protein yield (linear, P = 0.0264). The most effective rations for in vitro GP during different times differed between different measured parameters. Enzyme administration was more effective when the ratio of maize silage was increased.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Incubationsmentioning

confidence: 99%

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Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Elghandour¹,

Kholif²,

Molina³

et al. 2015

Turk J Vet Anim Sci

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“…This effect may be similar to that reported for direct-fed microbials and fermentation extracts (Varel et al 1993;Newbold 1995). Nsereko et al (2002) added various levels of an enzyme product from Trichoderma longibrachiatum to the diet of dairy cows and reported an increase in total viable rumen bacteria. Most of this increase was accounted for by increases in bacterial species that use cellobiose or glucose.…”

Section: Stimulation Of Rumen Microbial Populationsmentioning

confidence: 99%

Mode of action of exogenous cell wall degrading enzymes for ruminants

Beauchemin¹,

Colombatto²,

Morgavi³

et al. 2004

Can. J. Anim. Sci.

121

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Rode, L. M. 2004. Mode of action of exogenous cell wall degrading enzymes for ruminants. Can. J. Anim. Sci. 84: [13][14][15][16][17][18][19][20][21][22]. Recent studies have shown that adding exogenous fibrolytic enzymes to ruminant diets can increase milk production of dairy cows and weight gain of growing beef cattle as a result of enhanced feed digestion. While much progress has been made in terms of advancing feed enzyme technology for ruminants, considerable research is still required to develop more effective enzyme products. The mode of action whereby exogenous enzymes improve digestion of plant cell wall is complex, and there is evidence for numerous potential modes of action suggesting they are interdependant. A mode of action that accounts for the most critical factors that explain the observed increases in feed digestion is presented. Adding exogenous enzymes to the diet increases the hydrolytic capacity of the rumen mainly due to increased bacterial attachment, stimulation of rumen microbial populations and synergistic effects with hydrolases of ruminal microorganisms. The net effect is increased enzymic activity within the rumen, which enhances digestibility of the total diet fed. Thus, improvements in digestibility are not limited to the dietary component to which the enzymes are applied, which explains why fibrolytic enzymes can be effective when added to supplement or grain. The magnitude of the improvements in feed digestibility reported in some research studies using feed enzymes suggests a viable future for enzyme products in commercial ruminant production systems. A more complete understanding of the mode of action of these products will allow development of low-cost enzyme products designed specifically to improve feed digestion by ruminants. Des études récentes montrent que l'addition d'enzymes fibrolytiques exogènes à la ration des ruminants accroît la production des vaches laitières et le poids gagné par les bovins de boucherie à cause d'une meilleure digestion des aliments. Bien que la technologie des enzymes alimentaires destinés aux ruminants ait progressé, il reste encore beaucoup à faire avant qu'on arrive à des produits enzymatiques plus efficaces. La manière dont les enzymes exogènes facilitent la digestion de la paroi cellulosique est particulièrement complexe et tout indique que les nombreux modes d'action potentiels sont interdépendants. Les auteurs proposent un mode d'action qui tient compte des principaux facteurs expliquant la meilleure digestion des aliments observée. L'addition d'enzymes exogènes à la ration accroît la capacité d'hydrolyse du rumen principalement à cause d'une meilleure fixation des bactéries, d'une stimulation de la microflore du rumen et de la synergie avec les hydrolases des microorganismes. Il s'ensuit une plus grande activité enzymatique dans le rumen, ce qui améliore la digestibilité de la ration. Cette meilleure digestibilité ne vient donc pas uniquement de la composante alimentaire à laquelle s'attaquent les enzymes, ce qui explique l'efficacité de...

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“…This lack of effect of enzyme treatment on DML following in vitro fermentation has been recognised by others (Colombatto et al 2003) and may be a result of increased microbial biomass. Total bacterial numbers in ruminal fluid have been seen to increase with increasing enzyme levels (Nsereko et al 2002), and since DML in this experiment was in fact apparent DML, due to microbial biomass adhering to the residue, an increase in microbial mass due to enzyme treatment is conceivable.…”

Section: * Significant Quadratic Response (P0·05)mentioning

confidence: 62%

The effect of enzyme treatment on thein vitrofermentation of lucerne incubated with equine faecal inocula

et al. 2005

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A series of experiments was conducted to determine the effects of a fibrolytic enzyme preparation (enzyme 1; E1) on the in vitro fermentation of lucerne incubated with equine faecal inocula. In experiment 1, high-temperature-dried (HT) lucerne was treated with five levels of E1 (0 to 2·4 ml/g DM) and incubated at 508C for 20 h. Samples then received a simulated foregut digestion (SFD) treatment before DM and NSP analysis. In experiment 2, HT lucerne was treated with the same enzyme levels used in experiment 1. Samples were then split into two groups; plus or minus an SFD treatment before in vitro fermentation using an equine faecal inoculum. In experiment 3, fresh and wilted lucerne were treated with the same levels of E1 as experiments 1 and 2, incubated at 508C for 20 h, then fermented in vitro. For experiment 4, fresh and wilted lucerne were treated with low levels (0 to 0·008 ml/g DM) of E1 before fermentation. E1 significantly (P,0·05) enhanced DM and NSP losses from HT lucerne following SFD treatment compared with the control. High levels of E1 significantly (P,0·05) enhanced the rate, but not extent, of fermentation of HT, wilted and fresh lucerne; however, low levels of E1 were ineffective. At higher application levels, E1 appears to have considerable potential to enhance the nutritive value of lucerne for horses. Information on the fermentation kinetics of the substrates was valuable; all end-point measurements showed no effect of enzyme treatment. Enzyme treatment: In vitro fermentation: Equine inocula: LucerneThere is increasing interest in feeding high-energy, fibre-based feeds as an alternative to feeding cereal grains as a means of meeting the energy demands of working horses. This is due to the association of the onset of metabolic disorders, such as laminitis and colic, with feeding high levels of cereal grains. Preparations of fibrolytic enzymes have the potential to initiate the hydrolysis of forage fibre (structural carbohydrates), rendering the fibrous fraction more amenable to degradation in vivo. Exogenous fibrolytic enzymes have been reported to enhance the nutritive value of forages fed to ruminants by enhancing the digestibility of plant structural carbohydrates, thus enhancing the overall energy balance in the animal (Beauchemin et al.

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Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows

Cited by 118 publications

References 18 publications

Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios

Mode of action of exogenous cell wall degrading enzymes for ruminants

The effect of enzyme treatment on thein vitrofermentation of lucerne incubated with equine faecal inocula

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