Effect of a Garcinia gardneriana (Planchon and Triana) Zappi hydroalcoholic extract on melanogenesis in B16F10 melanoma cells

Campos, Patrícia Mazureki; Horinouchi, Cíntia Delai da Silva; Prudente, Arthur Silveira; Cechinel‐Filho, Valdir; Cabrini, Daniela de Almeida; Otuki, Michel Fleith

doi:10.1016/j.jep.2013.03.079

Cited by 32 publications

(23 citation statements)

References 27 publications

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“…GB-2a was more potent than kojic acid, a well-known depigmenting agent, because GB-2a concentrations were in nanomolars (nM) compared with the micromolars (µM) of kojic acid. This is in accordance with the previously observed hydroalcoholic extract of Garcinia gardneriana, which showed a decrease of melanogenesis (Mazureki Campos et al, 2013).…”

Section: Discussionsupporting

confidence: 93%

“…The leaves, barks and roots are the most used parts and these can be prepared as infuses, decocts or macerates, individually or in combination with other natural products (Corrêa, 1978;Castardo et al, 2008). Our previous study evidenced that the hydroalcoholic extract of Garcinia gardneriana decreased the amount of melanin in the B16F10 melanoma cells and, specifically, promoted an inhibition of tyrosinase activity (Mazureki Campos et al, 2013).…”

mentioning

confidence: 99%

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Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis

Campos

Prudente

Horinouchi

et al. 2015

Journal of Ethnopharmacology

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis

Campos

Prudente

Horinouchi

et al. 2015

Journal of Ethnopharmacology

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“…Cell growth/viability assay. The assay is based upon the cleavage (reduction) of the yellow tetrazolium salt MTT to give purple formazan crystals by mitochondrial reductase in viable cells (Campos et al, 2013;Zhou et al, 2013). Cells were seeded in a 96-well plate at a density 5 × 10 3 cells/well for B16F10 and 1 × 10 4 /well for A549, cultured for 24 h before the compounds were added to the medium.…”

Section: Methodsmentioning

confidence: 99%

Artepillin C and Other Herbal PAK1-blockers: Effects on Hair Cell Proliferation and Related PAK1-dependent Biological Function in Cell Culture

et al. 2015

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PAK1 (RAC/CDC42-activated kinase 1) is the major oncogenic kinase, and a number of herbal PAK1-blockers such as propolis and curcumin have been shown to be anti-oncogenic and anti-melanogenic as well as anti-alopecia (promoting hair growth). Previously, we found several distinct PAK1-inhibitors in Okinawa plants including Alpinia zerumbet (alpinia). Thus, here, we tested the effects of these herbal compounds and their derivatives on the growth of cancer or normal hair cells, and melanogenesis in cell culture of A549 lung cancer, hair follicle dermal papilla cell, and B16F10 melanoma. Among these herbal PAK1-inhibitors, cucurbitacin I from bitter melon (Goya) turned out to be the most potent to inhibit the growth of human lung cancer cells with the IC50 around 140 nM and to promote the growth of hair cells with the effective dose around 10 nM. Hispidin, a metabolite of 5,6-dehydrokawain from alpinia, inhibited the growth of cancer cells with the IC50 of 25 μM as does artepillin C, the major anti-cancer ingredient in Brazilian green propolis. Mimosine tetrapeptides (MFWY, MFYY, and MFFY) and hispidin derivatives (H1-3) also exhibited a strong anti-cancer activity with the IC50 ranging from 16 to 30 μM. Mimosine tetrapeptides and hispidin derivatives strongly suppressed the melanogenesis in melanoma cells.

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“…These data are in accordance with other partial structure of similar compounds in comparison with literature data (Hano et al 1993;Komguem et al 2005;Yang et al 2007) and were identified as a uncommon 1,5,8-trihydroxy-4 0 ,5 0 -dimethyl-2H-pyrane (2,3:3,2)-4-(3-methylbut-2-enyl) xanthone (2). Extract, fractions and compounds 1 and 2 were tested for their antiproliferative activity against different human cell lines (Table 3), since previous studies with another species of this genus exhibited promising melanogenesis in B16-F10 murine melanoma cells (Campos et al 2013). As shown in Table 3, the dichloromethane (DCM) was the most active tested fraction, demonstrating an interesting profile for most human cancer cell lines, with most active for glioma (U-251), ovary expressing multi-drugs resistance phenotype (NCI/ADR-RES), kidney (786-0) and prostate (PC-3) with total grown inhibition (TGI) values of 24.9, 28.7, 22.9 and 25.7 mg/mL, respectively.…”

Section: Resultsmentioning

confidence: 99%

In vitro antiproliferative activity of uncommon xanthones from branches of Garcinia achachairu

Mariano

Vendramini–Costa

Ruiz

et al. 2015

Pharmaceutical Biology

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Context Garcinia achachairu Rusby (Clusiaceae) popularly known as 'achachairu' is used in folk medicine to treat rheumatism, inflammation, pain and gastric disorder. Objective The present study investigated the chemical profile and antiproliferative effects of the methanolic extract, fractions and two xanthones, against some carcinoma cell lines in vitro. Materials and methods The compounds were isolated and identified by chromatographic and spectroscopic methods. The extract, fractions and compounds were tested human tumour cell lines of U-251 (glioma), MCF-7 (breast), NCI/ADR-RES (ovary expressing multi-drug resistance phenotype), 786-0 (kidney), NCI-H460 (lung, non-small cells), PC-3 (prostate) and HT-29 (colon), non-tumour cell line HaCat (human keratinocytes) in doses of 0.25-250 mg mL À 1 for 48 h. The antiproliferative activity was determined by spectrophotometric quantification (at 540 nm) of the cellular protein content using sulphorhodamine B assay. The prediction of parameters involved in the molecular bioavailability was executed directly by ChemDoodle (version 5.0.1) software (iChemLabs, LLC, Somerset, NJ). Results 3-Demethyl-2-geranyl-4-prenylbellidypholine (1) and 1,5,8-trihydroxy-4 0 ,5 0 -dimethyl-2H-pyrane (2,3:3,2)-4-(3-methylbut-2-enyl) xanthone (2), gartanin (3) and stigmasterol (4) were identified on the basis of spectroscopic techniques. Compounds 1 and 2 exhibited cytocidal activity, especially against breast, prostate and kidney cell lines, with TGI values of 15.8, 4.9, 9.1 and 39.4, 44.7, 40.9 mg/mL, respectively. Discussion and conclusion The presence of two sets of hydrophobic and hydrophilic groups in separate domains in each molecule might play a role in the mediation of tumour-specific action. Our data show that G. achachairu have potent antiproliferative action and should be considered an important source of potent anticancer compounds. ARTICLE HISTORY

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Effect of a Garcinia gardneriana (Planchon and Triana) Zappi hydroalcoholic extract on melanogenesis in B16F10 melanoma cells

Abstract: These results suggest that HEGG inhibition relates to its tyrosinase activity. Therefore, the hydroalcoholic extract of Garcinia gardneriana shows great potential for use as a depigmenting agent in hyperpigmentation disorders.

Cited by 32 publications

References 27 publications

Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis

Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis

Artepillin C and Other Herbal PAK1-blockers: Effects on Hair Cell Proliferation and Related PAK1-dependent Biological Function in Cell Culture

In vitro antiproliferative activity of uncommon xanthones from branches of Garcinia achachairu

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