Effect of a sugarcane cystatin on the profile and viability of microcosm biofilm and on dentin demineralization

Câmara, João Víctor Frazão; Araújo, Tamara Teodoro; Méndez, Daniela Alejandra Cusicanqui; Silva, Natara Dias Gomes da; Medeiros, Felipe Fávaro de; Santos, Lethycia Almeida; Carvalho, Thamyris de Souza; Reis, Fabiana Navas; Martini, Tatiana; Moraes, Samanta Mascarenhas; Shibao, Priscila Yumi Tanaka; Groisman, Sônia; Magalhães, Ana Carolina; Henrique-Silva, Flávio; Buzalaf, Marília Afonso Rabelo

doi:10.1007/s00203-021-02403-6

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…The viable cell counts in the microcosm biofilms of patients ranged from 5 to 8 log10 CFU for streptococci, 5 to 7 log10 CFU for mutans streptococci, 7 to 8 log10 CFU for lactobacilli and 0 to 9 log10 CFU for yeasts. Similar results were found by Vertuan et al [37], Câmara et al [38], and Mendéz et al [24], who obtained bacterial counts between 6 to 7 log10 CFU for streptococci, 5 to 7 log10 CFU for mutans streptococci and 6 to 7 log10 CFU for lactobacilli in microcosm biofilms from oral samples. Nonetheless, these authors did not investigate the presence of yeasts in the microcosm biofilms formed in their studies.…”

Section: Discussionsupporting

confidence: 87%

Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

et al. 2021

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Photodynamic therapy (PDT) mediated by Fotoenticine® (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium Streptococcus mutans. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of mutans streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.

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Section: Discussionsupporting

confidence: 87%

Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

et al. 2021

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“…25 As mentioned above, several intracellular proteins delivered to saliva after exfoliation of oral mucosa cells have the potential to bind to hydroxyapatite, or participate in supramolecular aggregates that bind to the precursor proteins in the AEP. This has also been observed in other recent studies, [26][27][28][29][30][31] and could have a central role in protecting the tooth surface against acid dissolution. Hence, it is worthwhile noting that not only do secreted salivary proteins participate in AEP formation, but oral mucosa proteins, gingival crevicular fluid and even bacteria also act strongly on the enamel pellicle.…”

Section: This Process Is Based On the Epigenetic Mechanisms Associate...supporting

confidence: 83%

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

MARTINI,

RIOS,

DIONIZIO

et al. 2023

Braz. oral res.

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The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calciumbinding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution.

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“…However, we did not nd similar results for dentin in our study, using a concentration of 0.1 mg/ml. Lower concentrations of Cane CPI-5 (0.025 and 0.05 mg/ml) have already been tested in microcosm bio lm for root dentin and they were not able to reduce the CFU counting for any bacterial group evaluated neither to reduce demineralization compared to negative control (PBS) [34].…”

Section: Discussionmentioning

confidence: 99%

Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin caries development

de Souza,

Braga,

Vertuan

et al. 2024

Preprint

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Purpose To evaluate the influence of radiation as well as of new formulations of artificial saliva on the development of root caries lesions. Methods Bovine root samples were divided into: irradiated (70 Gy) dentin or not; the type of biofilm (from irradiated or non-irradiated patients) and the type of artificial saliva (for the condition irradiated dentin/biofilm): Saliva A (inorganic); Saliva A + 1mg/ml hemoglobin; Saliva A + 0.1mg/ml cystatin; Saliva A + hemoglobin + cystatin; Bioextra (positive control) and water (negative control) (n = 12/group). Biofilm was produced using human biofilm and McBain saliva (0.2% of sucrose, 37o C and 5% CO2); the treatments were done 1x/day, for 5 days. Colony-forming units (CFU) counting was performed; demineralization was quantified by transversal microradiography. Two-way ANOVA/Bonferroni or Sidak test for the comparison between biofilm x dentin and ANOVA/Tukey or Kruskal-Wallis/Dunn for comparing artificial saliva were done (p < 0.05). Results The type of biofilm had no influence on CFU and demineralization. Sound dentin under control biofilm presented the lowest Lactobacillus ssp. and Streptococcus mutans CFU and the lowest mean mineral loss (R) (25.6 ± 2.2; 23.7 ± 2.9%) compared to irradiated dentin (26.1 ± 2.8; 28.1 ± 3.3, p < 0.004) for both types of biofilms (irradiated and no irradiated, respectively). Bioextra was the only one that reduced R (10.8 ± 2.5%) and LD (35 ± 15µm) compared to water (17.3 ± 3.3%, 81 ± 18µm, p < 0.0001). Conclusion Irradiation of dentin has impact on caries development; none of the experimental saliva was able to reduce its occurrence.

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Effect of a sugarcane cystatin on the profile and viability of microcosm biofilm and on dentin demineralization

Cited by 12 publications

References 31 publications

Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin caries development

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