Effect of Acridine Orange in Promoting Growth and Physiological Characteristics of Fragaria Ananassa Duch Under Salinity Stress in Vitro

Abstract

This experimental were conducted out at plant tissue culture lab., Center of Desert studies to test the effect of four concentrations of Acridine Orange viz., 0, 10, 50 and 100mM L−1 under four levels of salinity viz., 0, 30, 60 and 90 mM NaCl L−1 on some phenological traits including: leaves number, branches number, plant length and fresh weight. Membrane stability index and electrolytic leakage also were in vitro measured. The experiment was factorial and arranged in a complete randomized design (CRD) with five replicates. Results revealed that 50 mML−1 of Acridine orange gave highest leaves number of 14.67 leaves explant−1, branches number of 3.75 branches explants−1, plant length of 1.48cm and fresh weight of 0.71g. whereas, 10 mM L−1 membrane stability index of 76.63. Furthermore, 30 mML−1 possesses highest leaves number of 11.75 leaves explants−1, branches number of 2.83 branch explant−1, plant length of 1.29cm and fresh weight of 0.58g. The highest level of salinity (90 mM) gave highest of electrolyte of 69.07.It could concluded that 50 m mol l−1 of acridine orange was the best concentration to promote growth of strawberry in vitro. Moreover, Plantlets were more tolerant to 30 m mol L−1 of Nacl in vitro.

Effect of Adding Organic Fertilizer and Humic Acid on some Vegetative and Root Growth Characteristics of Fig Seedlings, Diyala Black Cultivar

“…In addition, the role of humic acid (Table 3) leads to an increase in the amount of chlorophyll, which encourages the production of large quantities of carbohydrates, which are used in the various vital activities of the plant as shown in Table (7), this led to the improvement of all vegetative and root characteristics of fig seedlings and the absorption of a greater amount of nutrients from the soil, and this is consistent with what was stated by [16,17,18,19].…”

The Role of Organic Fertilizers and Humic Acid in some Characteristics of Vegetative and Root Growth and the Chemical Content of the Shoots of Fig Seedlings Cv. (Aswad Diyala)

“…In addition to the role of humic acid in maintaining soil moisture and aeration, which is reflected in the formation of a strong root system capable of absorbing nutrients and then stimulating vegetative growth [17], the reason for the increase in the nutrients content of the branches (table 5,6,7) may be due to the role of organic fertilizer and humic acid in increasing the permeability of cell membranes, which leads to facilitating the transfer of nutrients and thus increasing the efficiency of the plant to absorb and accumulate nutrients in the branches [18]. This is consistent with what was stated by Prajapati and Modi [19] and Kelley and Khalaf [20]and [21].…”

Effect of Organic and Chemical Fertilizers and Spraying with a Growth Stimulator on Some Chemical Properties of Vegetative Growth and Fruits of the Strawberry Plant Fragaria×ananassa

“…[17] reported that the acidity in fruits decreased in organically grown plants or plants that were fed adequate integrated nutrition. Poultry manure contains a good the proportion nitrogen and phosphorus, which leads to an increase in vegetative growth through an increase in the formation of proteins, and nucleic acids,the building of protoplasm, and efficiency in carbon metabolism, and the consequent increase in manufactured compounds and an increase in the percentage of dissolved solids [18] These results agreed with [19,20,21,22]…”