Effect of actinomycin D on the multiplication of the infectious pancreatic necrosis virus of trout

Bl, Nicholson

doi:10.1007/bf02136741

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Infectious pancreatic necrosis virus replicates well at 1O-26°C, poorly at 4°C, and not at all at 30°C. Virus replication is suppressed if infected cells are exposed to actinomycin D or ultraviolet irradiation within 2 hr after infection, but there is no effect if the cells are exposed later in the infectious cycle (Dobos, 1977;Kelly and Loh, 1975;Nicholson, 1971b).…”

Section: Growth Cycle Of Infectious Virionsmentioning

confidence: 98%

Fish Viruses and Viral Infections

McAllister

1979

Comprehensive Virology

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Section: Growth Cycle Of Infectious Virionsmentioning

confidence: 98%

Fish Viruses and Viral Infections

McAllister

1979

Comprehensive Virology

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“…To prepare virus labeled in the protein moiety, the medium from cell monolayers was replaced with diluted MEM (1 volume of MEM without serum + 9 volumes of Earle balanced salt solution [EBSS]) containing either a 14C-labeled amino acid mixture at a final concentration of 1 gCi/ml or [35S]methionine at VOL. 21,1977 on September 26, 2020 by guest http://jvi.asm.org/ Downloaded from 244 DOBOS 5 ,uCi/ml. IPN virus was added at a low-input MOI (as above), and the cultures were incubated at 220C until advanced CPE was evident.…”

Section: Cellsmentioning

confidence: 99%

Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus

Somogyi

Dobos

1977

J Virol

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A study of virus-specific protein synthesis in infectious pancreatic necrosis virus-infected RTG-2 cells was undertaken to find a relationship between the coding capacity of the virus genome (two segments of double-stranded RNA of 2.5 x 106 and 2.3 x 106 molecular weight) and the sizes and relative amounts of polypeptides in the virion and in infected cells. The time course of virus-specific protein synthesis was followed by pulse labeling infected UV-irradiated cells with [35S]methionine and analyzing the labeled proteins by polyacrylamide gel electrophoresis followed by autoradiography. Three size classes of virus-specific polypeptides were synthesized, in the same relative proportion, throughout the infectious cycle, beginning 3 h postinfection. Their designation and molecular

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“…Viruses. The Dry Mills strain of IPN virus was originally provided by D. Locke of the Maine Department of Inland Fisheries and Game and was propagated in RTG-2 cells in this laboratory as described previously (10,11). This strain of IPN virus was isolated from an outbreak of IPN at the Dry Mills Hatchery, Gray, Maine, in 1967.…”

Section: Methodsmentioning

confidence: 99%

Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures

1973

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Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID50) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 108-2 to 108 4 TCID6O per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests. PI).

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Effect of actinomycin D on the multiplication of the infectious pancreatic necrosis virus of trout

Cited by 4 publications

References 12 publications

Fish Viruses and Viral Infections

Fish Viruses and Viral Infections

Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus

Immunofluorescent Study of the Replication of Infectious Pancreatic Necrosis Virus in Trout and Atlantic Salmon Cell Cultures

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