Effect of acute exposure to nitrite on physiological parameters, oxidative stress, and apoptosis in Takifugu rubripes

Gao, Xiaoyan; Fei, Fan; Huo, Huan Huan; Huang, Bin; Xue, Meng; Zhang, Tao; Liu, Baoliang

doi:10.1016/j.ecoenv.2019.109878

Cited by 35 publications

(15 citation statements)

References 64 publications

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“…This result is consistent with the study by Gao et al. (2020), who found that GPx activity was reduced significantly in Japanese Pufferfish that were exposed to 1‐mg/L nitrite for 48 h. Glutathione is an antioxidant molecule that plays an important role in cellular metabolism and free radical scavenging (De Matos and Furnus 2000). In our research, the GSH content was related to exposure duration and dose, exhibiting a trend of increasing first and then decreasing.…”

Section: Discussionsupporting

confidence: 92%

“…In the current study, CAT activity was activated by nitrite, showing a positive correlation with the nitrite concentration in the early stage of exposure but then decreasing at 24 h. Similar variation tendencies were observed in Japanese Pufferfish (Gao et al. 2020). By measuring the activity of SOD, we found that the activity had increased in the 1‐mg/L group and decreased in the 100‐mg/L group.…”

Section: Discussionsupporting

confidence: 88%

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Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes

et al. 2022

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To evaluate the effects of nitrite on the oxidative damage of blood cells of Grass Carp Ctenopharyngodon idella, the isolated hemocytes were exposed to nitrite (0, 1, 10, or 100 mg/L) for up to 24 h. Hemoglobin (Hb) and methemoglobin (MetHb) concentrations, reactive oxygen species (ROS) and malondialdehyde (MDA) levels, mitochondrial membrane potential (∆Ψm), and antioxidant enzyme activity were assayed to assess hematological parameters and the antioxidant defense mechanism. Results showed a remarkable decrease in Hb concentration with increasing nitrite concentration after a 24‐h exposure, while the MetHb concentration increased significantly in nitrite exposure groups. The levels of ROS, ∆Ψm, and MDA increased to varying degrees with increases in nitrite exposure concentration and time. The total antioxidant capacity, catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione content showed a trend of rising initially and then decreasing with prolonged exposure time. Superoxide dismutase (SOD) activity was higher in the 1‐mg/L nitrite exposure group and lower in the 100‐mg/L group than in the control. The relative messenger RNA expression ratios of cat, sod1, and gpx were up‐regulated significantly in the 1‐ and 10‐mg/L groups and then declined in the 100‐mg/L group. Therefore, it can be concluded that nitrite exposure activates the antioxidant defense mechanism of Grass Carp hemocytes and that the balance of oxidant–antioxidant homeostasis will be undermined by higher nitrite doses or longer exposure periods.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes

et al. 2022

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“…Of the various stress indicators, cortisol is a sensitive indicator of toxic effects in fish exposed to environmental stressors. Gao et al [1] reported a significant increase in the cortisol of puffer fish, Takifugu rubripes, exposed to nitrite, and they suggested that the stimulation was caused by the activation of the hypothalamic-pituitary-interrenal axis in response to stress induced by nitrite exposure. Jia et al [15] also suggested that nitrite exposure caused a significant increase in the cortisol of juvenile turbot, S. maximus.…”

Section: Discussionmentioning

confidence: 99%

“…Nitrite is a critical toxic substance for organisms in the aquatic environment that is generated in the process of bacterial nitrification of ammonia or the denitrification of nitrate [1]. Generally, nitrite presently occurs at low concentrations in the aquatic environments, but can significantly increase in high-density aquaculture or eco-friendly aquaculture systems, such as Recirculating Aquaculture Systems (RAS) and bio-floc technology (BFT) [2,3].…”

Section: Introductionmentioning

confidence: 99%

Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀

2022

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Nitrite concentrations can reach high levels in indoor aquaculture systems, thus it is vital to determine the nitrite tolerance of aquaculture fish species. Here, juvenile hybrid groupers (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀, Family: Serranidae) were exposed to waterborne nitrite at 0, 10, 20, 40, and 80 mg NO2−/L for 2 weeks. Nitrite exposure caused significant reductions in hematocrit and hemoglobin levels, significant increases in plasma calcium and plasma ALP levels, but had no significant effects on magnesium and total protein levels. Of the antioxidant responses investigated, SOD activity increased significantly in the liver and gills, but GST activity and GSH levels were significantly inhibited by nitrite exposure. Stress indicators, such as plasma cortisol and HSP 70 levels, were significantly stimulated by nitrite exposure. In brief, nitrite exposure over 20 mg NO2−/L had toxic effects and affected the hematological properties, antioxidant responses, and stress indicators of juvenile hybrid groupers.

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“…Oxidative stress is a biochemical response triggered by environmental challenges and exposure to pollutants (Monserrat et al, 2007; Stoliar & Lushchak, 2012). Recent studies have shown that nitrite toxicity induces oxidative stress in fish (Cheng et al, 2015; Gao et al, 2020; Jensen et al, 2015; Kim et al, 2019; Maltez et al, 2018). Intracellular production of reactive oxygen species (ROS; Sun et al, 2014) and reactive nitrogen species (RNS) can increase after exposure to nitrite (Jensen & Hansen, 2011).…”

Section: Introductionmentioning

confidence: 99%

Acute toxicity and sublethal effects of nitrite on oxidative stress in early juvenile Brazilian flounder, Paralichthys orbignyanus

Miranda

Maltez

Campello

et al. 2021

Aquaculture Research

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This study evaluated the acute toxicity and sublethal effects of nitrite on oxidative stress parameters in early juvenile Brazilian flounder, Paralichthys orbignyanus. Fish were exposed to six concentrations of nitrite: 20, 40, 80, 170, 330 and 550 mg NO2−N‐L−1, plus a control with no added nitrite, all in triplicate for 96 h. Every 24 h, the tanks were monitored to check possible mortality to estimate the LC50. At the end of the experiment, fish were sampled for stress oxidative analysis. All fish exposed to 550 mg NO2−N‐L−1 died within the first 24 h. The LC50 ranged from 427.8 to 393.1 mg NO2−N‐L−1 for 24 and 96 h respectively. The total antioxidant capacity against peroxyl radicals (ACAP) showed a gradual reduction in fish exposed to nitrite levels from 170 mg NO2−N‐L−1. The glutathione S‐transferase (GST) activity was higher in 80, 170 and 330 mg NO2−N‐L−1. The non‐proteic (NPSH) and proteic (PSH) thiols levels were higher in 170 and 330 mg NO2−N‐L−1 compared with the control, 20 and 40 mg NO2−N‐L−1. Lipid peroxidation (LPO), as indicated by the thiobarbituric acid reactive substances (TBARS) level, was significantly higher in fish exposed to 330 mg NO2−N‐L−1 than the control and 20 mg NO2−N‐L−1. Moreover, a pro‐oxidant condition was induced when P. orbignyanus were exposed to concentrations from 80 mg NO2−N‐L−1, which was evidenced by alterations in the antioxidant system and increased levels of LPO. Therefore, it is recommended that the nitrite concentration do not exceed 40 mg NO2−N‐L−1 in order to avoid oxidative stress for early juvenile Brazilian flounder.

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Effect of acute exposure to nitrite on physiological parameters, oxidative stress, and apoptosis in Takifugu rubripes

Cited by 35 publications

References 64 publications

Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes

Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes

Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀

Acute toxicity and sublethal effects of nitrite on oxidative stress in early juvenile Brazilian flounder, Paralichthys orbignyanus

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