Effect of acute oral cadmium on mitochondrial enzymes in rat tissues

Rao, P. V. V. Prasada; Sridhar, M.K.C.; Desalu, A. B. O.

doi:10.1007/bf01059405

Cited by 18 publications

(4 citation statements)

References 20 publications

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“…Cadmium taken up by the liver parenchymal cells is known to be concentrated primarily in the mitochondria [21,22] and to disturb some mitochondrial functions. For instance, some components in the respiratory chain on the mitochondrial inner membranes and enzymes in the tricarboxylic acid cycle in the mitochondrial matrix have been observed to be inhibited by cadmium [23][24][25][26]. Such effects possibly result from the uptake of cadmium by mitochondria through the calcium-transport system [27,28].…”

Section: Discussionmentioning

confidence: 99%

A cadmium-binding protein in rat liver identified as ornithine carbamoyltransferase

Aoki

Sunaga

Suzuki

1988

Biochemical Journal

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A cadmium-binding protein of Mr about 40,000 (40K Cd-BPa) was detected in rat liver by Western blotting [Aoki, Kunimoto, Shibata & Suzuki (1986) Anal. Biochem. 157, 117-122]. It was characterized and identified as ornithine carbamoyltransferase (OCTase, EC 2.1.3.3) on the basis of coincidence of their physicochemical and enzymological features. The amino acid sequence of the N-terminal and those of three tryptic digests in 40K Cd-BPa were identical with those of OCTase. The Mr values of the denatured and native forms of 40K Cd-BPa (39,000 and 110,000 respectively) were the same as those of OCTase. 40K Cd-BPa showed, as OCTase activity, a specific activity of 230 mumol/min per mg of protein and Km of 0.6 mM for ornithine, this value also being essentially the same as that for OCTase. A rabbit antibody against OCTase reacted with 40K Cd-BPa. The native form of 40K Cd-BPa bound to 0.8 molar equiv, of cadmium, with a dissociation constant of 7.6 x 10(-6) M.

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Section: Discussionmentioning

confidence: 99%

A cadmium-binding protein in rat liver identified as ornithine carbamoyltransferase

Aoki

Sunaga

Suzuki

1988

Biochemical Journal

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“…Total lipids in general and phospholipids in particular in muscle, gill and liver, while free fatty acid concentrations in muscle and liver tissues increased (Murty & Devi, 1982;Rao & Rao, 1984a, b). Lipid content of brain tissue was significantly decreased in Cirrhinus mrigala which had been exposed to the carbamate insecticide carbaryl (Rao et al, 1984b).…”

Section: Lipidsmentioning

confidence: 96%

“…Since phospholipids are the most actively degradable lipids, it is suggested that they are rapidly utilized to provide energy. Therefore, a decrease in total phospholipids as observed when Oreochromis mossambicus were exposed to methyl parathion (Rao & Rao, 1984a, b) could be attributed to their use of energy to resist a stressor. Therefore, in addition to total lipid content and tissue-specific lipid content, we suggest that phospholipid profiling may be a useful monitoring tool to determine intermediate term toxicant-induced stress effects in fish.…”

Section: Lipidsmentioning

confidence: 99%

“…Endosulfan, an organochlorine insecticide, caused a significant increase in protein content of both kidney and brain tissue of C. punctatus exposed in vivo to concentrations ranging from 3.5 to 6.5 pg/l for 96 h (Murty & Devi, 1982). Alternatively, when the fish Cirrhinus mrigala was exposed to 2 mg/l the carbamate insecticide carbaryl in vivo for 96 h, the total protein concentrations in brain, gill and kidney tissue all increased significantly (Rao et al, 1984a, b). Both of these studies indicate a disturbance in protein metabolism, however, one caused an increase, while the other caused a decrease.…”

Section: Proteinmentioning

confidence: 99%

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Recent developments in and intercomparisons of acute and chronic bioassays and bioindicators

Giesy

Graney

1989

Hydrobiologia

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The ultimate goal of toxicity testing is to monitor or predict the effects of single compounds, elements or mixtures on the long-term health of individual organisms, populations, communities and ecosystems. Unfortunately, one does not always have all of the information required to determine the long-term or 'chronic' effects of toxicants on the survival, growth or reproduction of aquatic organisms. For this reason, the chronic effects of toxicants are often inferred or estimated from observations made during short-term or 'acute' field or laboratory studies, which may be conducted at greater concentrations of toxicant. The observations made in the short-term studies are then related to the chronic effects by some statistical relationship. There are basically two approaches: 1)The long-term effects on a parameter, such as survival (lethality) are predicted from observations on the same parameter, during short-term exposures; 2) Alternatively, the response of one parameter to long-term exposures of a toxicant can be predicted from the short-term responses of a different parameter. In this report we present several different examples of both types of methods for estimating chronic responses from information on more short-term responses and discuss the rationale, advantages and disadvantages of each. We also report on two biochemical indicators; energetic substrates and RNA/DNA ratio. These indicators both act as sensitive, integrative measures of sublethal effects of contaminants during both acute and chronic exposures.

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Uptake of trace elements by water lettuce (Pistia stratiotes)

Sridhar

1988

Acta hydrochim. hydrobiol.

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Summury: PiJtiu strutiotes L. obtained from a eutrophic lake in Ibadan, Nigeria was examined for its mineral and trace element composition. The study indicated that the shoot system accumulated more potassium, calcium and magnesium, whereas the root system accumulated significantly more cadmium, chromium, cobalt, copper, iron, lead, manganese, mercury, nickel, sodium and zinc. The possible utilization of this aquatic plant for the treatment of polluted waters and industrial effluents is discussed.

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Effect of acute oral cadmium on mitochondrial enzymes in rat tissues

Cited by 18 publications

References 20 publications

A cadmium-binding protein in rat liver identified as ornithine carbamoyltransferase

A cadmium-binding protein in rat liver identified as ornithine carbamoyltransferase

Recent developments in and intercomparisons of acute and chronic bioassays and bioindicators

Uptake of trace elements by water lettuce (Pistia stratiotes)

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