Effect of administration of vitamins C and E on fertilization capacity of rats exposed to noise stress

Abstract

The aims of this study were to evaluate the effects of administration of Vitamins C and E on fertilization capacity in rats exposed to noise stress. 40 adult male rats were randomly divided into 5 equal groups. Group 1 as controls who were not exposed to noise and groups 2-5 exposed to noise with 90-120 dB intensity and 300-350 Hz frequency from 7 pm to 7 am everyday for 50 days. Group 2 exposed to noise and did not receive Vitamins. Group 3 received vitamin C, Group 4 received Vitamin E. Group 5 received Vitamins C and E concomitantly. After 50 days, serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH) and testosterone were calculated. Then each rat was left with three female rats for mating. Pregnant females were sacrificed on the 19 th day of pregnancy and evaluated for the presence and number of viable, dead and absorbed fetuses. The level of FSH, LH and testosterone significantly decreased in rats exposed to noise (P < 0.05). By administration of Vitamins in groups 3-5 we observed that the level of hormones significantly increased in compared to group 2 (P < 0.05). The fertilization capacity of male rats in groups 3-5 significantly increased in compared to group 2 (P < 0.05). There was significant difference between groups 1 and 2 in case of fertilization capacity (P = 0.001). The data in this study strongly suggests a negative role for noise stress on level of FSH, LH and testosterone level and also fertilization capacity of male rats. To complement the information it is suggested that this research be done on human samples.

Przewlekła ekspozycja na hałas a niedobór testosteronu — metaanaliza i metaregresja wyników badań na gryzoniach

“…The study of Fathollahi et al [36] which deals with the effects of noise-induced stress on male rat fertility and T levels, although meeting inclusion criteria was not included because its design (same timeframe, description of animals and exposure, aims, findings and scientific writing) are exceptionally similar to those of Saki et al [33]. This fact creates a doubt that the two papers are actually reporting different studies.…”

“…Considerable changes were not observed in either the pooled Hedges's g or the heterogeneity, except when Saki et al [33] was excluded, which resulted in a two-fold drop in the effect and some decrease in the heterogeneity.…”

“…Due to limited full-text access, for the study of Rajabzadeh et al [25] we imputed the exposure based on previous work of the authors with a similar design. It should be noted that for the meta-analysis we considered only those experimental groups which did not receive other interventions, and disregarded combined exposures of noise and formaldehyde vapour [31], diazepam [32], vitamins C and E [33] or honey and vitamin E [25]. However, for Armario & Castellanos [30], we had to include the combined effect of noise and light exposures, which should be borne in mind.…”

“…All studies used validated and reliable methods (ELISA, RIA) to measure serum T levels. Some carried out histomorphological and microscopic analyses on the testes, assessed sperm parameters or pregnancy in female rats [31][32][33][34], but those outcomes were outside this study's scope.…”

“…Given that the study of Saki et al [33] received the highest quality score, the quality indices of all other studies were computed in comparison to it. From Figure 1, the pooled effect size was g = -2.41, suggesting a very large effect; that is, the exposed group had serum T levels more than two standard deviations lower than those in the control group.…”