Effect of aeration and substrate moisture content on sporulation of Metarhizium anisopliae var. acridum

Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

“…However, when yield was referred to as conidia per gram of wheat bran, independently to the amount of texturiser, yields did not present significant differences among the mixtures, with average production close to 1 x 10 10 conidia/gds (Table 1). This behavior was similar to a report by Arzumanov et al (2005), who described that in SSF using ground rice and sugar cane bagasse (50:50), Metarhizium anisopliae grew almost exclusively on rice particles, and the yield of conidia per gram of total dry matter was lower than that obtained in SSF with only rice as a substrate. Inoculum levels: It is convenient for SSF systems to know the effect of inoculum size on the final values for any relevant parameter.…”

“…For instance, t 10 was 285 h using the lowest inoculum (1 x 10 6 conidia/gds); however, this value could be reduced to 20% and 50% for higher inocula, since values for t 10 were 232 and 148 h using 7 x 10 6 or 5 x 10 7 conidia/gds, respectively. A level of 10 7 conidia/gds is commonly recommended as inoculum in the cultures of entomophogenic fungi (Dalla-Santa et al 2004;Arzumanov et al 2005). This result suggested that t 10 could be an appropriate criterion to define the harvest time for an entire batch, which in turn might reduce the maintenance and costs in the processes related to conidia production by entomophogenic fungi.…”

“…There are some key factors affecting the growth and conidial production in SSF and it is important to know their influence in the processes. Some of these main factors are temperature, pH, substrate (type and concentration), and moisture content (Barranco-Florido et al 2002;Larroche 1996;Arzumanov et al 2005). The aim of this work was to evaluate the effect of moisture content and inoculum size on the growth and conidia production by Beauveria bassiana using wheat bran as a substrate.…”

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

“…humidity of the solid substrate, which noticeably affects the sporulation process and is optimal between 57% and 58% RH (Arzumanov et al, 2005), pH, which needs to be slightly acidic in both phases to facilitate fungal growth and inhibit the growth of other microorganisms, and time (Kleespies and Zimmermann, 1992). In the mass production process, conidia are harvested 21 d after inoculation in the substrate; there have also been good results 14 d after inoculation (Rezende, 2009).…”

Pathogenicity of <i>Aspergillus clavatus</i> produced in a fungal biofilm bioreactor toward <i>Culex quinquefasciatus</i> (Diptera: Culicidae)

“…[11][12][13] By contrast, solid-state fermentation is a promising alternative since conidia and metabolites are generally produced in higher quantities when these microorganisms are produced on a solid substrate. [11][12][13][14][15][16] For example, Aspergillus oryzae has been reported to produce a 500-fold higher yield of recombinant chymosin in solid-state fermentation than in submerged fermentation. 17) In this context, efficient utilization of agro-industrial residues as carbon sources has been shown for the mass production of entomopathogenic fungi.…”