Effect of aerobic exercise and different levels of fine particulate matter (PM2.5) on pulmonary response in Wistar rats

Qin, Fei; Xu, Minxiao; Wang, Zhongwei; Han, Zhining; Dong, Yanan; Zhao, Jiexiu

doi:10.1016/j.lfs.2020.117355

Cited by 14 publications

(10 citation statements)

References 48 publications

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“…2a, d, g). The results found in this work were consistent to the previous investigations [15,17,27,29]. Noted that the pulmonary histopathological injury was irreversible, the long-term exposure to PM 2.5 may pose severe threat on the respiratory system to human beings since the pollution is persistent.…”

Section: Pm 25 -Induced Lung Injury In Micesupporting

confidence: 92%

Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms: from histopathology to integrated transcriptome analysis

Dai

Yang

et al. 2020

Preprint

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Background & aimsSome epidemiological investigations have revealed airborne fine particulate matter (PM2.5) could induce adverse effects on respiratory system of human. However, the experimental evidences of the harmful effect of PM2.5 from mid-scale city in China and associated molecular mechanisms was still scarce. In this study, we aimed to evaluate the adverse effect on the lung system of PM2.5 collected from mid-city of China and elucidate the underlying molecular mechanism through mRNA-seq and microRNA-seq integrated analysis.MethodsWe exposed male Balb/C mice to PM2.5 collected from Baoji city, China for 8 weeks (298.52 ± 25.86 μg/m3) using a whole-body exposure system. Micro-CT and histopathological analyses were performed to determine the morphology and histopathology changes of lung tissues induced by PM2.5. Transcriptome (both mRNA and microRNA) sequencing and the immunohistochemistry assay were performed to reveal the associated underlying mechanisms. The contents of PAHs absorbed in the PM2.5, as well as the pearson correlation index between it and the target genes and microRNA were examined.ResultsObvious lung injury including pulmonary dysfunction, immunological responses, and fibrosis were observed from the PM2.5 exposure group. The content of leucocyte of lung tissue was significantly increased. PM2.5 mainly induced immune pathways including B cell receptor signaling and cell adhesion molecules (CAMs). The expression levels of the associated genes and microRNA were verified using RT-qPCR. The protein expression of CD19, CD81, and PIK3CD involved into B cell receptor signaling significantly increased in exposure group. The concentrations of benzo(a)pyrene (BaP) showed a marked correlationship with the genes and microRNA. ConclusionsPM2.5 from the mid-scale city of China caused adverse effects on lung system of mice, which was much stronger than the mega city, indicating the health risk of the air pollution in the developing area should be paid more attention. The toxicity of PM2.5 may associated with immune system response. This work for the first time provided a new sight into the molecular mechanism of PM2.5 toxicity on lung system using transcriptomics integrated analysis and help further to develop effective measures to prevent air pollution associated lung disease, especially in the developing area in China.

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Section: Pm 25 -Induced Lung Injury In Micesupporting

confidence: 92%

Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms: from histopathology to integrated transcriptome analysis

Dai

Yang

et al. 2020

Preprint

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“…SOD and CAT regulate oxidative stress and free radicals, MDA reflects the degree of oxidative stress and T-AOC reflects the total antioxidant level of all antioxidant stress indexes (39-41). aerobic intermittent training decreases expression levels of Mda and inflammatory factors in serum and increases those of CAT and GSH (42). The concentration of GSH peroxidase, Glu and Mda in the serum of athletes increases along with expression levels of TnF-α, whereas those of caT decrease following anti-gravity exercise (43).…”

Section: Discussionmentioning

confidence: 99%

Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats

Zhu

et al. 2021

Mol Med Rep

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Moderate exercise is beneficial to physical and mental health. When the amount of exercise and exercise intensity exceeds a certain limit and reaches the state of exhaustion, oxidative stress levels in the body increase, which can lead to oxidative stress-associated damage. Lycium barbarum polysaccharide (lBP) is one of the primary active ingredients extracted from wolfberry. Following exhausting exercise in rats, lBP supplements decrease damage to the myocardium and blood vessels, indicating that lBP exerts a protective effect on the cardiovascular system. The Kelch-like ecH-associated protein 1 (Keap1)/nF-e2-related factor 2 (nrf2) anti-oxidative stress signaling pathway improves total oxidizing ability; anti-apoptosis and other aspects serve a vital role. in the present study, lBP intervention was performed in vivo and in vitro to observe its effect on the Keap1/nrf2 pathway and oxidative stress-associated indicators in order to clarify its protective mechanism. For the in vivo experiments, 60 male Sprague-dawley rats were randomly divided into normal control and aerobic, exhaustive and exhaustive exercise + lBP (200 mg/kg/day) groups. For the in vitro experiments, a rat thoracic aortic endothelial cell (rTaec) oxidative stress model was established using angiotensin ii (angii) and divided into blank control, lBP (3,200 µg/ml), angii (1x10 -4 mol/l) and angii + lBP groups. For in vitro experiments, small interfering (si)rna (50 nmol) was used to transfect rTaec and induce gene silencing of nrf2. eliSa, hematoxylin and eosin staining, Tunel, immunofluorescence, western blotting, immunohistochemistry and reverse transcription-quantitative Pcr were used to evaluate and verify the effect of lBP on oxidative stress indicators and the expression of Keap1/nrf2 antioxidative stress signaling pathway. The in vivo experiments showed that lBP decreased the expression of serum malondialdehyde (Mda) and angii, as well as apoptosis of blood vessels and cardiomyocytes and expression of TnF-α in rats following exhaustive exercise. Meanwhile, lBP enhanced expression of the Keap1/nrf2 signaling pathway and downstream associated protein glutamyl-cysteine synthetase catalytic subunit (Gclc), quinone oxidoreductase 1 (nQo1) and glutamate-cysteine ligase modified subunit (GCLM) in the thoracic aorta and myocardium of rats following exhaustive exercise. in rTaec in vitro, lBP decreased the expression of Mda and TnF-α in the supernatant, promoted the nuclear translocation of nrf2 and increased expression levels of Gclc, nQo1 and GclM. Following sinrf2 transfection into endothelial cells, the anti-inflammatory and antioxidant stress effects of lBP were decreased. lBP was found to enhance the expression of the Keap1/nrf2 antioxidant stress signaling pathway in endothelial cells, decreasing oxidative stress and the inflammatory response. Moreover, lBP improved the antioxidant stress ability of endothelial cells and alleviated injury of myocardial vascular tissue, thereby protecting the cardiovascular system.

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“…In addition, work by Qin et al found that aerobic exercise was associated with a reduction in the adverse pulmonary effects of PM2.5 in Wistar rats, underscoring the bene cial cardiorespiratory effects of such exercise. [31] Qigong practices are also thought to alter blood viscosity and vascular elasticity, thus improving microvascular circulation in practitioners. [32] Previous research has shown that lung cancer patients practicing GLQG exhibit improvements in microcirculation.…”

Section: Discussionmentioning

confidence: 99%

Effect of supervised aerobic exercise on the physical and mental health of lung cancer patients

Ma¹,

Zhong²,

Wang³

et al. 2020

Preprint

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Background The present study was designed to evaluate the impact of aerobic exercise and climate on cardiopulmonary fitness, overall physical health, and mental health in non-small cell lung cancer (NSCLC) patients. Methods Between 2014 and 2019, a total of 111 NSCLC patients with stage IIIB and stage IV disease that had undergone standard treatment were separated into two experimental groups: a control group that conducted stretching exercises, and a test group that practiced Guolin qigong (GLQG). Of these patients, 56 remained in Heilongjiang Province and 55 went to Hainan Province during a 4-month period in the winter. Interventions were practiced three times per week. Cardiorespiratory fitness was the primary study outcome, whereas secondary outcomes included changes in anxiety, depression, dyspnea, immune functionality, and quality of life (QOL). Results Patients in the Heilongjiang Province cohort that had practiced GLQG exhibited significant improvements in cardiopulmonary functionality, depression, and QOL (social well-being, functional well-being, and lung cancer subscale scores) relative to the control group ( P <0.05). Similarly, in the Hainan Province cohort, the patients that had practiced GLQG exhibited significant improvements in their cardiorespiratory fitness, dyspnea, depression, and QOL relative to controls ( P <0.05). Following a 4-month intervention period, we additionally detected significant differences between patient groups in both provinces with respect to both mean albumin-to-globulin ratio (AGR) and neutrophil-to-lymphocyte ratio (NLR) values ( P <0.05). The intervention cohort in Hainan Province exhibited significant improvements in cardiorespiratory fitness (VO2MAX, FEV1/FVC (%), FEV1% pred, and FVC % pred), dyspnea, and QOL ( P hysical and emotional well-being subscales) relative to the intervention cohort in Heilongjiang Province ( P <0.05). Conclusions GLQG and a more favorable climate are both associated with significant improvements in the QOL, immune functionality, and cardiorespiratory function of NSCLC patients.

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Effect of aerobic exercise and different levels of fine particulate matter (PM2.5) on pulmonary response in Wistar rats

Cited by 14 publications

References 48 publications

Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms: from histopathology to integrated transcriptome analysis

Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms: from histopathology to integrated transcriptome analysis

Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats

Effect of supervised aerobic exercise on the physical and mental health of lung cancer patients

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