Effect of Aerobic Exercise on Intestinal Microbiota with Amino Acids and Short-Chain Fatty Acids in Methamphetamine-Induced Mice

Abstract

This study aimed to investigate the changes in intestinal homeostasis and metabolism in mice after methamphetamine (MA) administration and exercise intervention. In this study, male C57BL/B6J mice were selected to establish a model of methamphetamine-induced addiction, and the gut microbiota composition, short-chain fatty acids (SCFAs), and amino acid levels were assessed by 16S rRNA, liquid chromatography–tandem mass spectrometry, and gas chromatography–tandem mass spectrometry, respectively. The results showed that 23 dominant microbiota, 12 amino acids, and 1 SCFA were remarkably higher and 9 amino acids and 6 SCFAs were remarkably lower in the exercise model group than in the control group. Among the top 10 markers with opposite trends between the exercise intervention group and model group, the differential microbiomes included Oscillibacter, Alloprevotella, Colidextribacter, Faecalibaculum, Uncultured, Muribaculaceae, and Negativibacillus; amino acids included proline; and SCFAs included isovaleric acid and pentanoic acid. Proline was negatively correlated with Negativibacillus and positively correlated with pentanoic acid. The results suggested that moderate-intensity aerobic exercise may modulate changes in the composition of the gut microbiota and the levels of amino acids and SCFAs induced by MA administration.

Urolithin A Ameliorates Athletic Ability and Intestinal Microbiota in Sleep Deprivation from the Perspective of the Gut‐Muscle Axis

“…Furthermore, Muribaculaceae , sensitive to sleep restriction, [ 32 ] is also involved in the muscle functions of moderate‐intensity aerobic exercise. [ 33 ]…”

Multiomics reveals the mechanism of B. longum in promoting the formation of mixed-species biofilms

“…57 Amino acids are the source of short-chain fatty acids (SCFAs) that can provide energy for the colonization and maturity of mixed-species biofilms. [58][59][60] SCFAs produced by Porphyromonas gingivalis and Fusobacterium nucleatum have been reported to promote the formation of biofilms with some other commensal strains; Pseudomonas aeruginosa has also been reported to diffuse further and colonize a series of biofilms formed by proteobacteria through the production of SCFAs; 61 Pseudomonas also uses vitamin B12 to synthesize methionine and ribonucleotides in the process of biofilm formation through an oxygendependent pathway. 62 Amino acids have also been reported to have a direct effect on the formation of biofilms.…”

Effects of acupuncture on gut microbiota and short-chain fatty acids in patients with functional constipation: a randomized placebo-controlled trial

“…However, it should be noted that butyric acid has a bidirectional regulatory effect on intestinal motility, promoting intestinal motility at low doses but inhibiting it at high doses (Parthasarathy et al, 2016), which may partly explain the two-way effect of acupuncture in FC patients. Furthermore, other SCFAs did not change significantly after acupuncture, suggesting that factors such as diet and exercise may also influence the levels of these SCFAs (Seethaler et al, 2022;Liang et al, 2023). Therefore, further research is needed to fully elucidate the effect of acupuncture on the regulation of microbial metabolic pathways in the gut of FC patients.…”