Effect of age on the susceptibility of zebrafish eggs to industrial wastewater

From cutting edge to guideline: A first step in harmonization of the zebrafish embryotoxicity test (ZET) by describing the most optimal test conditions and morphology scoring system

“…The pores have been reported to potentially restrict the uptake of compounds depending on their size, as could be shown for fluorescent dextrans exceeding 3 kDa [25]. There is limited evidence that the permeability of the chorion changes during the embryonic development and that, after hardening, the chorion is less permeable [26]. It then functions as a barrier for even smaller molecules for the ZF embryo [27].…”

Influence of the perivitelline space on the quantification of internal concentrations of chemicals in eggs of zebrafish embryos (Danio rerio)

“…The hardness of the chorion increases within the first hour of development and it was assumed that this turns the chorion into a barrier against the uptake of pollutants (Gellert and Heinrichsdorff, 2001). Therefore standard fish embryo test guidelines require to expose ZFE very early in their development before the hardening of the chorion (OECD, 2014).…”

Toxicity Evaluation of Pig Slurry Using Luminescent Bacteria and Zebrafish

“…And then the samples were stored at 4 °C until used. Before the toxicity experiments, all samples were diluted, where de-ion water was used in luminescence experiment, and standard dilution water [23] was used in zebrafish experiment.…”