Effect of agitation and frequent subculturing on recovery of aerobic and facultative pathogens by Roche Septi-Chek and BACTEC blood culture systems

Kim, M J; Gottschall, Richard L.; Schwabe, L D; Randall, Eileen L.

doi:10.1128/jcm.25.2.312-315.1987

Cited by 25 publications

(12 citation statements)

References 6 publications

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“…The agitation principle has been implemented in the processing of clinical blood cultures during the last 15 years, and experience has generally been positive (1,6,8,10,12,14,15,16,19,21,22). One reason why it has taken such a long time to adopt the agitation principle with respect to clinical blood cultures may be that many of the traditional visual signs of growth (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Several large-scale studies concerning the advantages of agitation have been performed, but they are difficult to interpret because of simultaneous variations in other parameters, e.g. blood volume and blood culture system (10,21,22).…”

Section: Discussionmentioning

confidence: 99%

“…In the mid-l970s, Ellner et al adopted this principle with respect to clinical blood cultures (6). During the last 15 years, several studies have been published concerning the advantages of agitation for detection speed and/ or yield (1,6,8,10,12,14,15,16,19,21,22).…”

mentioning

confidence: 99%

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Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Arpi

Larsen

1992

APMIS

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Arpi, M. & Olesen Larsen, S. Comparison of detection speed and yield in agitated and non-agitated aerobic blood culture bottles. APMIS 100: 1061-1065, 1992.The influence of agitation on detection speed and yield was evaluated in 7,033 paired, identical aerobic blood culture bottles (Media Department, Statens Seruminstitut, Copenhagen, Denmark). One bottle was agitated and the other was incubated stationary. Of a total of 943 organisms isolated in aerobic blood culture bottles, 74% were clinically significant. No difference in the total yield of significant organisms was observed between agitated and non-agitated bottles. In the evaluation of detection speed, only cultures where organisms were isolated from both bottles, but at different times, were included, to ensure that no other factors influenced the result. Staphylococci, Pseudomonus spp., and Candidu spp. were detected significantly faster in agitated bottles, on average 0.5-1 day earlier (p < 0.05), and in the majority of the cases within the first incubation day. These species are frequently found and are important causes of severe generalized infections, especially in immunocompromised patients, where early detection is of great importance. The detection principle in agitated bottles in our system, darkening of blood, proved to be an easy, reliable, and fast method to detect positive aerobic blood cultures, which could probably lead to increased automation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Arpi

Larsen

1992

APMIS

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“…Several studies have shown that agitation of aerobic vials increases the speed of different blood culture systems (9,11,15,28). The beneficial effect of agitation can probably be explained by a better oxygenation of the media, more even distribution of nutrients, and separation of bacteria from each other and from the leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Comparison between Bactec Nr. 660® and a conventional 12‐tube blood culture system

Nir

Prag

Jensen

et al. 1990

APMIS

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Comparison between Bactec Nr. 660" and a conventional 12-tube blood culture system. APMIS 98: [645][646][647][648][649][650][651] 1990.The detection power of the automated blood culture system Bactec NR 660@, based on infrared detection of carbon dioxide in an agitated aerobic medium and a non-agitated anaerobic medium, was compared with that of our conventional 12-tube blood culture system. Of 1685 paired blood cultures, 258 (15.3%) were positive in one or both systems. Clinically relevant isolates were found in 1 1.5%. The dominating species were Escherichia coli (4 1 %), followed by Staphylococcus aureus (14%) and Klebsiella spp. (8%). The Bactec" system detected 178 (10.6%) and the 12-tube system 157 (9.3%) clinically relevant microorganisms after seven days' incubation. Significantly more clinically relevant isolates were detected by the Bactec" system alone as compared with the conventional system alone (40 versus 19, p < 0.01). The detection time was significantly shorter in the Bactec" system for all isolates and for E. coli and S. aureuS separately (p < 0.0 I). 1.89' 0 of the isolates in the Bactec" system and 2.19' 0 in the 12-tube system were considered clinically non-relevant contaminants.

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“…This was most likely due to the presence of reducing agents in the Signal medium (i.e., sodium pyruvate, sodium thioglycolate, cysteine hydrochloride, and dithiothreitol). Previous studies (3,6) suggested that the recovery of aerobic organisms could be improved with the use of agitation and terminal subcultures. However, we were unable to confirm this with the Signal system.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of the oxoid signal and Roche Septi-Chek blood culture systems

et al. 1988

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The Oxoid Signal blood culture system (Oxoid USA, Inc., Columbia, Md.) was compared with the Roche Septi-Chek system (Roche Diagnostics, Div. Hoffmann-La Roche Inc., Nutley, N.J.), with the latter consisting of a tryptic soy broth (R-TSB) bottle with an attached agar slide unit and a Columbia broth bottle. A total of 5,034 cultures with equal volumes of blood in each bottle were processed. Overall, more organisms were recovered in the R-TSB bottle than in the Signal bottle, with significantly more aerobic organisms (Pseudomonas spp., Acinetobacter spp., and yeasts) recovered in the R-TSB bottles and anaerobes and viridans group streptococci recovered in Signal bottles. Approximately equivalent numbers of organisms were recovered in the Signal and Columbia broth bottles. The times of detection were essentially identical with the three blood culture broth systems. During the study, 30.6% of the Signal bottles had a positive indicator of growth, of which 1,103 (71.7%) were false-positive cultures. Additionally, nonviable organisms resembling streptococci were observed in 13.7% of the Signal bottles that were Gram stained and in unioculated blood culture bottles. With appropriate modifications of the preparation of the media, the latter problem can be eliminated.

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Effect of agitation and frequent subculturing on recovery of aerobic and facultative pathogens by Roche Septi-Chek and BACTEC blood culture systems

Cited by 25 publications

References 6 publications

Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Comparison between Bactec Nr. 660® and a conventional 12‐tube blood culture system

Comparative evaluation of the oxoid signal and Roche Septi-Chek blood culture systems

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