Effect of Agitation Speed for Enzymatic Hydrolysis of Tapioca Slurry Using Encapsulated Enzymes in an Enzyme Bioreactor

Rahim, Siti Noraida Abd; Sulaiman, Alawi; Hamid, Ku Halim Ku; Edama, Nurul Aini; Baharuddin, Azhari Samsu

doi:10.7763/ijcea.2015.v6.447

Cited by 12 publications

(4 citation statements)

References 14 publications

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“…In another study, 150 rpm of agitation gave the highest yield of reducing sugar from POME [28] and PKC [18]. The agitation speed is an important parameter for mixing efficiency to raise productivity [30]. Purwanto et al [29] reported that the lower and higher speed decreased the productivity of the enzymatic hydrolysis process due to the sensitivity of the enzyme to mechanical shear stress.…”

Section: Effect Of Agitationmentioning

confidence: 99%

“…The interface of the parameters, those that were narrowed down through PBD, is optimized considering the quadratic, interaction, and linear effects in the treatment under CCD. Several researchers have utilized these optimization methods to evaluate the maximum production of reducing sugar [30]. From the OFAT studies, the significant factors were imperiled to an optimization process in the form of FCCCD under the RSM method to obtain the optimum enzymatic hydrolysis conditions for maximal reducing sugar yield.…”

Section: Effect Of Phmentioning

confidence: 99%

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Acid–Base Pretreatment and Enzymatic Hydrolysis of Palm Oil Mill Effluent in a Single Reactor System for Production of Fermentable Sugars

Deb

Alam

Rahman

et al. 2023

International Journal of Polymer Science

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Palm oil mill effluent (POME) is one of the main agro-industrial wastewaters in Malaysia. Highly polluting POME is a serious threat to the environment. In recent years, the methods used to treat POME are inefficient and complex in terms of cost or environmental preservation. The main object of this research is to propose a single reactor system (SRS) obtained from POME wastewater discharge as a promising low-cost treatment and high-energy method for harvesting the fermentable sugar by applying acid–base–enzyme pretreatment and hydrolysis of POME by locally produced cellulase enzymes to enhance biofuel production. Several experiments were conducted to produce fermentable sugars through the statistical methods, including the characterization of POME, acid-base pretreatment, and enzymatic hydrolysis process for reducing sugar production. The one-factor-at-a-time (OFAT) results showed that the highest reducing sugar yield, 23.5 mg/mL of POME, was achieved by enzymatic hydrolysis in an SRS without having a separation and purification. Based on OFAT performance, optimization of two factors such as substrate concentration (total suspended solids, TSS %w/v) and enzyme loading (μmol/min) was carried out by applying face-centered central composite design (FCCCD) under the response surface methodology (RSM) to develop a second-order regression model. The optimum reducing sugar production was 26.6 mg/mL (53.14%) with the conditions of 5% w/v, TSS, and 80 μmol/min/mL of the enzyme dose. In addition, the results of this research can be further considered in biofuel production using other wastewaters to enhance biofuel production as well as wastewater treating functions and minimize the negative environmental impacts.

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Section: Effect Of Agitationmentioning

confidence: 99%

Section: Effect Of Phmentioning

confidence: 99%

Acid–Base Pretreatment and Enzymatic Hydrolysis of Palm Oil Mill Effluent in a Single Reactor System for Production of Fermentable Sugars

Deb

Alam

Rahman

et al. 2023

International Journal of Polymer Science

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“…The mixing efficiency plays an important role in the optimization process of a bioreactor because enzymes are sensitive to mechanical shear stress. Therefore, excessive and lower agitation speed can reduce the efficiency of hydrolysis process (Abd Rahim, Sulaiman, Hamid, Edama, & Baharuddin, 2015). Agitation intensity of the culture broth has multiple effects on microorganisms, such as rupturing of the cell wall, alteration in the fungal morphology, fluctuation in the growth rate and efficiency which in turns affect the rate of desire product formation (Purwanto, Ibrahim, & Sudrajat, 2009).…”

Section: Optimization Of Different Factors For Enhanced Production Of...mentioning

confidence: 99%

Optimization and production kinetics for cellulases by wild and mutant strain of Thermomyces dupontii in stirred tank reactor

et al. 2022

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The main bottleneck in fermentation technology is scaling up procedure of industrial enzymes according to the biological characteristics of the organism. The current study describes the production kinetics of cellulases in stirred tank reactor by using mutant and wild strains of T. dupontii. The fermentation span of both the strains in bioreactor was examined. It is was found in mutant strain of T. dupontii fermentation time required for optimum production was reduced to 48h as compared to 72h in wild strain. The kinetic studies also exhibited greater value of µ (h-1) in case of mutated strain in comparison with wild strain. The effects of some other critical factors like agitation intensity dissolve oxygen, aeration, temperature, size of inoculum and pH was estimated on enzyme production kinetics. The results shows maximum activity of cellulases was attained at 220 rpm, 15% dissolve oxygen, aeration rate 1.5 vvm, 55°C, 8 % inoculum size and pH 5.5 for both strains respectively. The higher values of enzyme production kinetics i.e. Yp/x, Qp, Qx and qp in STR in case of mutant strain indicates its superiority over wild strain of T. dupontii. Thus mutant thermophilic T. dupontii might be a potential candidate for industrial applications.

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“…Effect of reaction time on the production of CD using CGTase from Bacillus licheniformis was conducted at various reaction times of 1, 2, 3, 4 and 5 hr (Rahim et al 2015). About 20 ml of soluble potato starch solution with concentration of 6% (w/v) was prepared in 50 mM sodium phosphate buffer (pH 6.0) by gelatinize in a steam water bath for 10 min and the solution was allowed to cool at room temperature.…”

Section: Reaction Timementioning

confidence: 99%

Effect of Reaction Parameters on the Production of Cyclodextrin using Cyclodextrin Glucanotransferase from Bacillus licheniformis

Man

Shaarani@Nawi

Arshad

et al. 2020

JCEIB

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Cyclodextrin glucanotransferase (CGTase) is an industrial enzyme that converts starch into cyclodextrin (CD). CD has many potential applications in wide range of industries including food, cosmetic, pharmaceutical and plastic industries. Production of CD from CGTase is based on a few process parameters such as concentration of substrate, types of substrate, reaction time, pH and temperature. In this present study, the effect of types of substrate (corn, potato, wheat, rice and tapioca starch), concentration of substrate (1, 2, 3, 4, 5, 6, 7, 8 and 9%) and reaction time (1, 2, 3, 4 and 5 hr) on the CD production using commercial CGTase from Bacillus licheniformis were studied. Among the five types of substrate tested, the potato starch was the most suitable substrate for the production of CD with 10.24 mg/ml of CD. In addition, by using 6% (w/v) of potato starch, the production of CD was 15.16 mg/ml. The optimal reaction time was found to be 4 hr with 12.41 mg/ml of CD. Therefore, the best reaction parameters proved to be valuable for the highest CD production. Hence, CGTase from Bacillus licheniformis was demonstrated to produce high CD at optimum reaction conditions and it has potential to be used in large scale production for the industrial purposes.

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Effect of Agitation Speed for Enzymatic Hydrolysis of Tapioca Slurry Using Encapsulated Enzymes in an Enzyme Bioreactor

Cited by 12 publications

References 14 publications

Acid–Base Pretreatment and Enzymatic Hydrolysis of Palm Oil Mill Effluent in a Single Reactor System for Production of Fermentable Sugars

Acid–Base Pretreatment and Enzymatic Hydrolysis of Palm Oil Mill Effluent in a Single Reactor System for Production of Fermentable Sugars

Optimization and production kinetics for cellulases by wild and mutant strain of Thermomyces dupontii in stirred tank reactor

Effect of Reaction Parameters on the Production of Cyclodextrin using Cyclodextrin Glucanotransferase from Bacillus licheniformis

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