Effect of alcohol dehydrogenase 1C (ADH1C) genotype on  vitamin A restriction and marbling in Korean native steers

Peng, Dong Qiao; Jung, Usuk; Lee, Jae Sung; Kim, Won Seob; Jo, Yong Ho; Kim, Min Jeong; Oh, Young Kyun; Baek, Youl Chang; Hwang, Seong Gu; Lee, Hong Gu

doi:10.5713/ajas.16.0708

Cited by 12 publications

(11 citation statements)

References 18 publications

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“…The negative relationship between serum vitamin A and beef marbling score during the fattening stage was firstly observed in Japanese Black steers 1 . After that, numerous studies have been conducted using vitamin A restriction strategy to increase the marbling score of different species of cattle/steers [2][3][4] . Under vitamin A restriction during the fattening stage, some metabolic biomarkers in different species of cattle/steers were found, including higher levels of serum glucose, urea nitrogen, albumin, and magnesium in Japanese Black steers 5 , and the increasing level of albumin, urea nitrogen, creatinine, and non-esterified fatty acid in Korean native steers 6 .…”

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confidence: 99%

Metabolomics analyses to characterize metabolic alterations in Korean native calves by oral vitamin A supplementation

Peng

Kim²,

Lee

2020

Sci Rep

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previous studies have reported that vitamin A administration in the birth stage of calves could promote preadipocyte and muscle development. However, the metabolic change after vitamin A administration remains unknown. Thus, the objective of this study was to perform metabonomics analyses to investigate the effect of vitamin A in Korean native calves. Ten newborn calves (initial average body weight: 30.4 kg [SD 2.20]) were randomly divided into two groups treated with or without vitamin A supplementation (0 IU vs. 25,000 IU vitamin A/day) for two months until weaning. Metabolic changes in the serum and longissimus dorsi muscle of calves were investigated using Gc-tof-MS and multivariate statistical analysis. As a result, ten metabolic parameters in the serum and seven metabolic parameters in the longissimus dorsi muscle were down-regulated in the vitamin A treatment group compared to those in the control group (VIP value > 1.0, p < 0.05). Both serum and longissimus dorsi muscle showed lower levels of cholesterol and myo-inositol in the vitamin A treatment group than in the control group (p < 0.05). These results indicate that vitamin A supplementation in the early growth period of calf could maintain the preadipocyte status, which can contribute to future adipogenesis in the intramuscular fat production of Korean native cattle.

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confidence: 99%

Metabolomics analyses to characterize metabolic alterations in Korean native calves by oral vitamin A supplementation

Peng

Kim²,

Lee

2020

Sci Rep

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“…The g.2352T > C SNP in the HSPB1 gene produced a significant difference in meat quantity, and the performance of animals with the CC homozygous genotype was higher than that of other genotypes (Suh et al, 2020). In a study into the production of high-quality beef, the CT heterozygote genotype of c.-64T > C SNP in the ADH1C gene was found to have an increased marbling score compared with the other genotypes (Peng et al, 2017). Oh et al (2012) suggested that the marbling score of animals with the GG homozygous genotype of the g.17924G > A SNP in the FASN gene was significantly increased over those of other genotypes.…”

Section: Association Of Identified Snps With Beef Quality and Quantit...mentioning

confidence: 99%

“…Using the c.-64T > C SNP of the ADH1C gene, Ward et al (2012) reported that marbling in cattle with the TT genotype of the ADH1C gene was higher than that in TC and CC genotypes during 5 months of restriction of vitamin A in Angus crossbred steers (Ward et al, 2012). Peng et al (2017) analyzed the association of the c.-64T > C SNP of the ADH1C gene with intramuscular fat deposition in Korean cattle during experiments with vitamin-A restriction in Korean cattle steers (Peng et al, 2017). The aim of this study was to establish a genotyping system based on AS-PCR analysis using three SNPs in the HSPB1, ADH1C, and FASN genes, to make genotyping more convenient and to reduce the amount of labor and time required.…”

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confidence: 99%

Allele-specific polymerase chain reaction for the discrimination of elite Korean cattle associated with high beef quality and quantity

Lee

Nam

Kim³

et al. 2022

Arch. Anim. Breed.

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Abstract. Techniques such as direct sequencing and PCR-RFLP (restriction fragment length polymorphism) are widely used to analyze the genotypes of livestock. However, these conventional methods have the disadvantage of taking a lot of time and incurring considerable cost. The allele-specific PCR method performs PCR using two primers, and a single nucleotide polymorphism (SNP) genotype can be identified through electrophoresis, saving time and cost. Highly accurate results can be obtained by designing specific primers according to the allele of the SNP under study, utilizing primer binding to a complementary matching sequence. In this study, we established a genotyping system with the AS-PCR technique, using SNPs related to the improvement of the meat quality and meat mass of Korean cattle. Using the PRIMER1 program, we designed specific primers for SNPs located at the 3′ end, with one SNP marker in the HSPB1 gene related to meat quantity and two SNP markers in the ADH1C and FASN genes related to meat quality in cattle. AS-PCR was performed on 10 Korean cattle using the primers designed with this system, and the genotypes could be identified by the size of the PCR product amplified as a result of electrophoresis. In the case of the HSPB1 g.2352T > C SNP, the T allele was amplified to 148 bp, and the C allele was amplified to 222 bp. The ADH1C c.-64T > C SNP was amplified to 492 bp at the T allele and 330 bp at the C allele. The FASN g.17924G > A SNP A allele was amplified to 377 bp and the G allele to 507 bp. The results for each SNP genotype were verified using direct sequencing, which showed that the genotypes identified by direct sequencing and the genotypes identified by the AS-PCR method matched exactly. The AS-PCR method therefore appears to be valuable for use in a genotyping system.

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“…The DNA was extracted from the hair follicle using the manual method 25 . Hair follicles were cut off 5-10 mm of 20 hairpieces and placed into a 1.5 mL tube.…”

Section: Dna Extraction From Hair Follicle For Snp Genotype Of Hsp70mentioning

confidence: 99%

Effects of Different Protein Levels on Growth Performance and Stress Parameters in Beef Calves Under Heat Stress

Kim

Nejad

Peng

et al. 2021

Preprint

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This study investigated the effects of dietary protein levels under various heat stress (HS) conditions on the growth performance and stress parameters in Korean native beef calves. Male calves (n = 40; initial BW = 202.2 ± 3.31 kg) were randomly assigned to climatic-controlled chambers with 3×3 factorial arrangements. Calves were assigned into three dietary protein levels (low protein; LP = 12.5%, medium protein; MP = 15%, and high protein; HP = 17.5%) and three stress levels (mild: temperature-humidity index (THI) = 74 to 76, moderate: THI = 81 to 83, and severe: THI = 89 to 91) with control (threshold: THI = 70 to 73 and dietary protein level 12.5%). The calves were subjected to ambient temperature (22 °C) for 7 days and subsequently to the temperature and humidity corresponding to the target THI level for 21 days. As a result, average daily gain (ADG) was decreased (P < 0.05) under severe HS level compared to the mild and moderate HS stress levels. However, HP increased ADG (P < 0.05) than moderate levels (LP) and severe levels (LP and MP). Under different HS levels (mild, moderate, and severe), HR, RT, and blood cortisol were increased compared to a threshold level, but no differences were observed in the parameters among various protein levels. Varied HS levels decreased the levels of blood glucose, NEFA, and amino acids (AAs) (lysine and glutamic acid) compared to a threshold (P < 0.05). But, the HP group resulted in increased levels of blood glucose, NEFA, and AAs (lysine and glutamic acid) compared to LP and MP groups under severe HS stress. The expression level of the HSP70 gene (P < 0.05) in PBMC and hair follicles was increased following an increase in moderate and severe HS levels. Also, HSP70 gene expression in the HP group was decreased compared with LP and MP groups under intense HS level. Overall, HS in Korean native beef calves exhibited negative effects on ADG, blood glucose, NEFA, and AA profile. However, 17.5% of dietary protein (HP) could compensate for the growth of heat-exposed Korean native beef calves through the regulation of homeostasis by protein and energy metabolism. Also, it was evident that adequate protein (HP) is used as a major nutrient for HSP70 synthesis in PBMC and hair follicles causing, a boost in the immune system of heat-exposed Korean native beef calves.

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Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers

Cited by 12 publications

References 18 publications

Metabolomics analyses to characterize metabolic alterations in Korean native calves by oral vitamin A supplementation

Metabolomics analyses to characterize metabolic alterations in Korean native calves by oral vitamin A supplementation

Allele-specific polymerase chain reaction for the discrimination of elite Korean cattle associated with high beef quality and quantity

Effects of Different Protein Levels on Growth Performance and Stress Parameters in Beef Calves Under Heat Stress

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