Effect of Alkali on Diphosphopyridine Nucleotide

Investigation of the open ring form of nicotinamide adenine dinucleotide

“…The stability of 2-hydroxynicotinaldehyde in 7.5 and 10 N NaOH was checked under the conditions used for the kinetic measurements, since this compound is known to be photolabile in strongly alkaline solutions (Kaplan et al, 1951) ation of 2% in readings was observed for the fluorescence, with the final fluorescence being essentially the same as the initial readings. Under the conditions of the experiment, 2-hydroxynicotinaldehyde is stable.…”

Reaction of Alkyl Isocyanides with Ozone. A New Isocyanate Synthesis1

“…The transient existence of pseudo bases has long been suspected in the formation of pyridones from N-alkylpyridinium halides10 and in the reaction of strong alkali with DPN,11 but there is very little evidence in support of this hypothesis. [10][11][12] In connection with the preparation of a number of 1 -alkyl-3-substituted-1,4-dihydropyridines as model compounds of DPNH,13 it was observed that the aqueous solution of the quaternary pyridinium halide and sodium carbonate became yellow before the introduction of the sodium dithionite. In the case of one compound, l-benzyl-3-acetylpyridinium chloride, a yellow solid also precipitated.…”

Synthesis of an oligonucleotide with a nicotinamide mononucleotide residue and its molecular recognition in DNA helices

“…In the cationic, oxidized form, lability to a base is a significant problem, while the reduced form reacts quickly under acidic conditions. 16,17 This was confirmed in exploratory reactions involving NADH, NAD + , and their pyrophosphate cleavage products. The labilities precluded a synthetic approach involving conventional chain assembly by solidphase DNA synthesis, which employs both acidic and strongly basic conditions.…”