Effect of all-trans retinoic acid on the barrier function in human retinal pigment epithelial cells

Rong, Junbo; Liu, Shuangzhen

doi:10.1016/j.bbrc.2011.03.080

Cited by 21 publications

(20 citation statements)

References 34 publications

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“…All-trans retinoic acid regulates TJ resistance in various cell types (29)(30)(31)(32). Tobioka et al (1996) reported that ATRA increased TER in rat peritoneal mesothelial cells (47).…”

Section: Discussionmentioning

confidence: 99%

Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

et al. 2015

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“…All-trans retinoic acid regulates TJ resistance in various cell types (29)(30)(31)(32). Tobioka et al (1996) reported that ATRA increased TER in rat peritoneal mesothelial cells (47).…”

Section: Discussionmentioning

confidence: 99%

Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

et al. 2015

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“…The effect of RA seems to depend on the sensitivity and origin of the epithelial cell type tested. Although some authors have reported that RA increases epithelial barrier in cell types such as retinal pigmentary (Rong and Liu, 2011), intestinal (Osanai et al, 2007; Baltes et al, 2004) or mammary epithelia (Marshall et al, 2009), a major finding in this work is the reversible modulation of epithelial barrier integrity exerted by RA. It was reported that RA modulates corneal epithelial differentiation and proliferation in a concentration-dependent manner, both in vivo and in vitro (Kim et al, 2012; Kruse and Tseng, 1994; Stonecipher et al, 1988; Tseng et al, 1984); likewise RA inhibited the increase of TER when RCE1(5T5) cells grew in its presence.…”

Section: Discussionmentioning

confidence: 79%

“…Considering that retinoids may modulate TJ in other epithelia (Rong and Liu, 2011; Hatakeyama et al, 2010; Telgenhoff et al, 2008), we tested the effect of different concentrations of all -trans retinoic acid (RA) on the development of TER along the growth and differentiation of RCE1(5T5) cells. Cells were plated as described (see Materials and Methods) and after the third day in culture, medium was changed to medium containing 0.1, 1.0 or 10 µM RA; control cultures were supplemented with medium without retinoid.…”

Section: Resultsmentioning

confidence: 99%

Expression of claudins -2 and -4 and cingulin is coordinated with the start of stratification and differentiation in corneal epithelial cells: retinoic acid reversibly disrupts epithelial barrier

et al. 2012

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SummaryAlthough tight junctions (TJ) have been extensively studied in simple epithelial cells, it is still unknown whether their organization is coupled to cell differentiation in stratified epithelia. We studied the expression of TJ in RCE1(5T5) cells, an in vitro model which mimics the sequential steps of rabbit corneal epithelial differentiation. RCE1(5T5) cells expressed TJ components which were assembled once cells constituted differentiated epithelia, as suggested by the increase of transepithelial electrical resistance (TER) which followed a similar kinetic to the expression of the early differentiation marker Pax-6. TJ were functional as indicated by the establishment of an epithelial barrier nonpermeable to ruthenium red or a biotin tracer. In immunostaining experiments, TJ were located at the superficial cells from the suprabasal layers; Western blot and RT-PCR suggested that TJ were composed of claudins (cldn) -1, -2, -4, cingulin (cgn), occludin (ocln) and ZO-1. Semi-quantitative RT-PCR and TER measurements showed that TJ became organized when cells began to form a 3–5 layers stratified epithelium; TER increased once cells reached confluence, with a time course comparable to the raise in the expression of cgn, cldn-2 and -4. Nevertheless, cldn-1, -2, ZO-1 and ocln were present in the cells from the beginning of cultivation, suggesting that TER increases mainly depend on TJ assembly. While EGF increased epithelial barrier strength, retinoic acid disrupted it, increasing paracellular flux about 2-fold; this effect was concentration dependent and completely reversible. Our results suggest that TJ assembly is tightly linked to the expression of corneal epithelial terminal phenotype.

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“…Based on detection in F9 cells, in a colitis model, and in some cancer tissues (29–31), RA is believed to be an obligatory component in the differentiation of epithelial cells that leads to the establishment of epithelial integrity. In their study, Rong and Liu (23) observed that the expression of ZO-1 and occludin increased in ARPE-19 cultures treated with atRA, suggesting that atRA has a barrier function in a process involving a specific increase in these TJ-associated proteins. Of note, in this study, the increase in the expression of ZO-1 and occludin in the eyes of guinea pigs with LIM was partly inhibited following the injection of LE540 into the vitreous chamber.…”

Section: Discussionmentioning

confidence: 95%

“…Recent studies have shown that RA may promote the function of the epithelial barrier, and its bioavailability regulates the epithelial barrier, which is accompanied by altering the expression of TJ-associated proteins (23). The aim of this study was to investigate the changes in the expression of TJ-associated proteins in the RPE-choroid complex in the eyes of guinea pigs with lens-induced myopia (LIM), and to investigate the effect of RA on the TJs of the RPE-choroid complex of guinea pigs in vivo .…”

Section: Introductionmentioning

confidence: 99%

Effect of retinoic acid on the tight junctions of the retinal pigment epithelium-choroid complex of guinea pigs with lens-induced myopia in vivo

Wang¹,

Liu²,

Mao³

et al. 2014

International Journal of Molecular Medicine

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Zonula occludens-1 (ZO-1) and occludin are important tight junction (TJ)-associated proteins, which are expressed in the retinal pigment epithelium (RPE)-choroid complex. Retinoic acid (RA) is a regulator of eye growth and may play an important role in forming functional TJs. The aim of this study was to detect the changes that occur in the expression of ZO-1 and occludin in the RPE-choroid complex of guinea pigs with lens-induced myopia (LIM), and to investigate the effect of RA on TJ-associated proteins in vivo. We developed an animal model of myopia by placing a −6.00 D negative lens on the right eyes of 3-week-old guinea pigs. The refractive error and axial length of the eye were measured on days 0, 3, 7 and 14. High-performance liquid chromatography (HPLC) was performed to detect the changes in endogenous RA in the RPE-choroid complex. The expression of ZO-1 and occludin was observed by immunofluorescence and assayed by western blot analysis. Additionally, 2 μl LE540 (2.5 μg/μl), an antagonist of RA receptors (RARs), was injected into the vitreous chamber of the eyes of guinea pigs with LIM and 2 μl phosphate-buffered saline (PBS) (2.5 μg/μl) were injected as a negative control. We observed no obvious change in RA, ZO-1 and occludin expression in the normal control group within 14 days. In the LIM and LIM plus PBS groups, the level of RA and the expression of ZO-1 and occludin in the RPE-choroid complex significantly increased within 14 days along with the development of myopia. However, the level of RA was inhibited and the expression of TJ-associated proteins decreased in the eyes of guinea pigs with LIM following the injection of LE540. Thus, we consider that the expression of ZO-1 and occludin is increased in the RPE-choroid complex during the development of myopia. This change in expression may be regulated by RA, a factor known to be involved in the regulation of eye growth.

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Effect of all-trans retinoic acid on the barrier function in human retinal pigment epithelial cells

Cited by 21 publications

References 34 publications

Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

Expression of claudins -2 and -4 and cingulin is coordinated with the start of stratification and differentiation in corneal epithelial cells: retinoic acid reversibly disrupts epithelial barrier

Effect of retinoic acid on the tight junctions of the retinal pigment epithelium-choroid complex of guinea pigs with lens-induced myopia in vivo

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