Effect of altering photocrosslinking conditions on the physical properties of alginate gels and the survival of photoencapsulated cells

Zhao, Delu; Tie, Chaorong; Cheng, Bo; Yang, Sisi; Wang, Xin; Sun, Zhen; Yin, Miaomiao; Zhu, Huaian; Yin, Miao

doi:10.1016/j.polymdegradstab.2020.109297

Cited by 17 publications

(17 citation statements)

References 39 publications

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“…The molecular weight of the macromer, the degree of cross-linking, and the chemical structure of the polymer affect its morphology. 15,18 Previous studies reported that a lower degree of alginate oxidation led to a lower density of hydrogel networks, 15,28 which is in accordance with the results of this study. In this study, the changes in the structure of alginate by the oxidation reaction may also have resulted in denser, interconnected network-like structures on POMA gels compared to PMA gels.…”

Section: Resultssupporting

confidence: 92%

“…Compared with PMA gels, POMA hydrogels displayed obviously faster swelling kinetics. Many factors, such as the macromer molecular weight and cross-linking density, impact the swelling behavior. , Compared to PMA gels, the decrease in the SR of POMA hydrogels may be due to oxidation-induced changes in the chemical structure of alginate and rapid degradation of the gels.…”

Section: Results and Discussionmentioning

confidence: 99%

“…However, due to the absence of related hydrolytic enzymes, photocross-linked alginate hydrogels (and ionic cross-linked alginate gels) degrade much more slowly in vivo in an unpredictable manner by releasing various molecular weight alginate strands and may not be completely degraded or removed from the body through physiological systems in terms of their high-molecular-weight alginate polymers, which seriously limits their applications in biomedicine. 11,15,16 According to previous research, it is known that the degradation rate of photocross-linked alginate hydrogels is related to many factors like the molecular weight of alginate, 17,18 macromer concentration, 9,18 photoinitiator concentration, 18 UV light intensity and exposure time, 18 and the degradation microenvironment. 19 Lueckgen et al 20 found that enzymatically degradable peptides grafted into photocrosslinked alginate hydrogels can accelerate hydrogel degradation.…”

Section: Introductionmentioning

confidence: 99%

“…According to previous research, it is known that the degradation rate of photocross-linked alginate hydrogels is related to many factors like the molecular weight of alginate, , macromer concentration, , photoinitiator concentration, UV light intensity and exposure time, and the degradation microenvironment . Lueckgen et al found that enzymatically degradable peptides grafted into photocross-linked alginate hydrogels can accelerate hydrogel degradation.…”

Section: Introductionmentioning

confidence: 99%

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Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Zhao

Wang

Cheng

et al. 2022

ACS Appl. Mater. Interfaces

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Photocross-linked alginate hydrogels, due to their biodegradability, biocompatibility, strong control for gelling kinetics in space and time, and admirable adaptability for in situ polymerization with a minimally invasive approach in surgical procedures, have created great expectations in bone regeneration. However, hydrogels with suitable degradation kinetics that can match the tissue regeneration process have not been designed, which limits their further application in bone tissue engineering. Herein, we finely developed an oxidation strategy for alginate to obtain hydrogels with more suitable degradation rates and comprehensively explored their physical and biological performances in vitro and in vivo to further advance the clinical application for the hydrogels in bone repair. The physical properties of the gels can be tuned via tailoring the degree of alginate oxidation. In particular, in vivo degradation studies showed that the degradation rates of the gels were significantly increased by oxidizing alginate. The activity, proliferation, initial adhesion, and osteogenic differentiation of rat and rabbit bone marrow stromal cells (BMSCs) cultured with/in the hydrogels were explored, and the results demonstrated that the gels possessed excellent biocompatibility and that the encapsulated BMSCs were capable of osteogenic differentiation. Furthermore, in vivo implantation of rabbit BMSC-loaded gels into tibial plateau defects of rabbits demonstrated the feasibility of hydrogels with appropriate degradation rates for bone repair. This study indicated that hydrogels with increasingly controllable and matchable degradation kinetics and satisfactory bioproperties demonstrate great clinical potential in bone tissue engineering and regenerative medicine and could also provide references for drug/growth-factor delivery therapeutic strategies for diseases requiring specific drug/growth-factor durations of action.

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Section: Resultssupporting

confidence: 92%

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Zhao

Wang

Cheng

et al. 2022

ACS Appl. Mater. Interfaces

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“…Photo-cross-linking is widely used for 3D bioprinting due to its cost effectiveness, its ability to be carried out at room or physiological temperature, and its comparative flexibility of precursor polymer types in that any material that can be functionalized with a free radical polymerizable group can be used . However, photo-cross-linking typically requires the use of cytotoxic photoinitiators or the introduction of cytotoxic photopolymerizable functional groups into bioinks as well as the use of low-wavelength light that can impact cell viability at certain wavelengths and intensities, while the free radicals produced by photoinitiators and UV light may have harmful effects on cells by damaging cell membranes, nucleic acids, and proteins . Only a few studies exist that systematically document these concerns, and the practical impact of any kind of photoinduced toxicity is found to be insignificant in many publications; however, concerns still do persist over the use of photo-cross-linking particularly for the bioprinting of more sensitive cell types or using higher-intensity/lower wavelength lights.…”

Section: Conventional Cross-linking Strategies For Hydrogel Bioinksmentioning

confidence: 99%

Click Chemistry Hydrogels for Extrusion Bioprinting: Progress, Challenges, and Opportunities

et al. 2022

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The emergence of 3D bioprinting has allowed a variety of hydrogel-based “bioinks” to be printed in the presence of cells to create precisely defined cell-loaded 3D scaffolds in a single step for advancing tissue engineering and/or regenerative medicine. While existing bioinks based primarily on ionic cross-linking, photo-cross-linking, or thermogelation have significantly advanced the field, they offer technical limitations in terms of the mechanics, degradation rates, and the cell viabilities achievable with the printed scaffolds, particularly in terms of aiming to match the wide range of mechanics and cellular microenvironments. Click chemistry offers an appealing solution to this challenge given that proper selection of the chemistry can enable precise tuning of both the gelation rate and the degradation rate, both key to successful tissue regeneration; simultaneously, the often bio-orthogonal nature of click chemistry is beneficial to maintain high cell viabilities within the scaffolds. However, to date, relatively few examples of 3D-printed click chemistry hydrogels have been reported, mostly due to the technical challenges of controlling mixing during the printing process to generate high-fidelity prints without clogging the printer. This review aims to showcase existing cross-linking modalities, characterize the advantages and disadvantages of different click chemistries reported, highlight current examples of click chemistry hydrogel bioinks, and discuss the design of mixing strategies to enable effective 3D extrusion bioprinting of click hydrogels.

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Copper ions‐photo dual‐crosslinked alginate hydrogel for angiogenesis and osteogenesis

Liu,

Ye,

et al. 2024

J Biomedical Materials Res

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Early healing of bone defects is still a clinical challenge. Many bone‐filling materials have been studied, among which photocrosslinked alginate has received significant attention due to its good biocompatibility and morphological plasticity. Although it has been confirmed that photocrosslinked alginate can be used as an extracellular matrix for 3D cell culture, it lacks osteogenesis‐related biological functions. This study constructed a copper ions‐photo dual‐crosslinked alginate hydrogel scaffold by controlling the copper ion concentration. The scaffolds were shaped by photocrosslinking and then endowed with biological functions by copper ions crosslinking. According to in vitro research, the dual‐crosslinked hydrogel increased the compressive strength and favored copper dose‐dependent osteoblast differentiation and cell surface adherence of rat bone marrow mesenchymal stem cells and the expression of type I collagen (Col1), runt‐related transcription factor 2 (Runx2), osteocalcin (OCN), vascular endothelial growth factor (VEGF). In addition, hydrogel scaffolds were implanted into rat skull defects, and more angiogenesis and osteogenesis could be observed in in vivo studies. The above results show that the copper‐photo‐crosslinked hydrogel scaffold has excellent osseointegration properties and can potentially promote angiogenesis and early healing of bone defects, providing a reference solution for bone tissue engineering materials.

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Effect of altering photocrosslinking conditions on the physical properties of alginate gels and the survival of photoencapsulated cells

Cited by 17 publications

References 39 publications

Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Degradation-Kinetics-Controllable and Tissue-Regeneration-Matchable Photocross-linked Alginate Hydrogels for Bone Repair

Click Chemistry Hydrogels for Extrusion Bioprinting: Progress, Challenges, and Opportunities

Copper ions‐photo dual‐crosslinked alginate hydrogel for angiogenesis and osteogenesis

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