The vertical connectivity of the river aquatic ecosystem was evaluated based on fish and artificial structures in Dorim stream, an urban stream in Seoul. As a result of a survey in the downstream area in 100.0 m of a total of 71 artificial structures, 13,728 individuals of fishes belonging to five orders, seven families, and 25 species were investigated, with the dominant species Zacco platypus and the subdominant species Rhynchocypris oxycephalus. As for endemic species, seven species were investigated and in terms of feeding characteristics, omnivorous species were the most common with 17 species (68%). Also an alien species, Poecilia reticulata was found. Fish species tended to decrease as the survey was conducted to upstream. Based on the movement characteristics of the fish species and the features of artificial structure survey results, the longitudinal continuity of each artificial structure was evaluated as 43 continuity, two damaged, 19 discontinuity, and seven absent. In inclined structures, stream velocity was found to be the main factor for discontinuity. In vertical structures, the down depth and head drop appeared to be the main factors for discontinuity. The results of this survey are expected to serve as basic data for the conservation of river aquatic ecosystems in the future.