Effect of Ammonium Nitrate on Antioxidants Production of Date Palm (Phoenix dactylifera L.)in vitro

Shehata, Wael F.; Aldaej, Mohammed I.; Alturki, Saleh M.; Ghazzawy, Hesham S.

doi:10.3923/biotech.2014.116.125

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…The optimum concentration of NH 4 NO 3 to KNO 3 ratio was 20.0 and 37.6 mM in the medium to produce the maximum amount of anthocyanin compared to control and other ratios. Medium with double strength of KNO 3 (37.6 mM) increased the anthocyanin production, while doubling the concentration of NH 4 NO 3 (40.0 mM) in the medium failed to enhance the anthocyanin content, since higher ammonium concentration in the medium increases the acidity of the medium which results in less consumption of potassium which directly influence the production of anthocyanin (Al-Ibresam and Al-Meer 2008). These results indicate that the increased ratio of KNO 3 with the basal level of NH 4 NO 3 in the medium had a positive effect on enhancement of anthocyanin content.…”

Section: Discussionmentioning

confidence: 99%

“…MS medium constitutes with inorganic metal compounds which plays the important role in growth, development of plant cells and also involves an in vitro production of secondary metabolites. Ammonium and potassium nitrate is one of the inorganic compounds which play a significant role in plant metabolic process for the production of anthocyanin and other secondary metabolites (Shehata et al 2014). Zahedzadeh et al (2015) reported that changing the ratio of ammonium to potassium nitrate into the MS medium resulted in a significantly higher accumulation of anthocyanin in apple.…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium

et al. 2018

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Anthocyanins are major water-soluble and dynamic colouring plant pigment present in plant tissues with the high antioxidant properties. The role of ammonium and potassium nitrate in the culture medium on anthocyanin augmentation is probed thoroughly, but the mechanism of its biosynthesis continues to be unclear. Hence, the present study was undertaken to optimise nitrate ratio in the culture medium for anthocyanin augmentation and examination of its biosynthesis pathway in callus culture of Daucus carota. MS basal medium fortified with various ratio of NH 4 NO 3 :KNO 3 was employed to find their impact on biomass, anthocyanin augmentation and the expression profile of anthocyanin biosynthesis genes in the callus culture. The data indicated that the highest anthocyanin content (9.30 ± 0.25 mg/100 g FW) was seen in callus grown on the medium supplemented with 20.0 mM NH 4 NO 3 :37.6 mM KNO 3 and the least was seen in the medium which contained 40.0 mM NH 4 NO 3 :18.8 mM KNO 3 (2.74 ± 0.27 mg/100 g FW). This indicates an optimal concentration of NH 4 NO 3 :KNO 3 ratio is essential to produce a higher amount of anthocyanin in in vitro culture. Meanwhile, anthocyanin biosynthesis genes were differentially expressed as confirmed by qRT-PCR in the time interval of 5, 10, 15, 20 and 25 days. The transcript levels of nine anthocyanin biosynthesis genes were increased in the response of varying NH 4 NO 3 :KNO 3 ratio in the medium. The transcript level of early genes PAL, 4CL, CHS and CHI increased by 19.5, 21.0, 16.2 and 9.98-fold, respectively, compared with control. In addition, late biosynthesis genes LDOX and UFGT resulted in the transcript level of 11.3 and 13.6-fold, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium

et al. 2018

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“…Date palm tissues are very susceptible to browning and elimination or minimization of this process is an essential prerequisite to successful culture establishment. Mustafa et al [22] and Shehata et al [27] proved in their studies the correlation between the phenolic production and browning intensity in date palm cultured in vitro . So, in this study we were able to minimize date palm tissue browning by incubated the culture at 5 °C for 45 and 75 days.…”

Section: Resultsmentioning

confidence: 99%

Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee’

Al-Mayahi

Ali

Shareef

2018

Journal of Genetic Engineering and Biotechnology

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Mass propagation of date palm through indirect somatic embryogenesis or organogenesis has attracted the interest of commercial producers. But, this technique still faces some problems that hindered the production of date palm plantlets in vitro. Tissue browning is one of the serious problems that reduce callus growth and shoot regeneration. So the objective of the present study is to investigate the effect of cold pretreatment on callus growth, shoot regeneration, and polyphenol oxidase (PPO) activity during the callus culture. Results showed that a high survival rate of callus cultures (100%) were obtained when cultures were incubated in low temperature (cold treatment) for 45 and 75 days. On the other hand, total amount on phenolic compounds was also reduced to 0.47 and 0.53 mg GAE/g after same period of incubation (45 and 75 days respectively) at low temperature. In additional, our results showed that the highest frequency of shoot formation (66.67 and 73.34, %) and the highest shoot numbers (7.8 and 8.6 shoots/100 mg) were obtained from callus treated with low temperature for 45 and 75 days, respectively.

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“…Free amino acid and total soluble protein contents of the plants treated with Beltanol and chloramphenicol at a high concentration of 100 mg/l decreased by up to twofold lower than those observed in the , 2012). Therefore, the decrease in the free amino acid and total soluble protein contents could be accounted for the delay of procallus emergence and the reduction of fresh and dry weights of procallus (Mustafa et al, 2013, Shehata et al, 2014. Biochemical analysis revealed that 50 mg/l chloramphenicol did not stimulate any negative effect of procallus on 2, 4-D and dicamba auxin.…”

Section: The Effect Of Decontamination Agents On Date Palm Procallus mentioning

confidence: 99%

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

Abass¹,

Al-Utbi²,

Al-Samir³

2016

Aust J Crop Sci

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This study was conducted to evaluate the phytotoxic effects of different antibiotics and fungicides on the procallus of date palm (Phoenix dactylifera L.). The fungicides Switch® and Beltanol and the antibiotics chloramphenicol and gentamycin were used in this study. Results confirmed the lethal effects of Switch®, gentamycin, and chloramphenicol (at 100 mg/l in dicamba treatment) on date palm tissues. The treated shoot tips did not develop into procallus, and their growth was completely inhibited. Beltanol treatments significantly decreased the biochemical parameters of the procallus treated with 2, 4-D and dicamba. In the Beltanol-treated tissues, the initial period of callus induction was prolonged from 63.33 and 69.66 days to 97.66 and 117.66 days. The callus induction percentage of the plants treated with the decontamination agents was also decreased. The fresh and dry weights of the procallus and the browning percentage and intensity of the cultured tissues in Beltanol and chloramphenicol treatments (100 mg/l) increased compared with those in the control treatments. Total carbohydrates, free proline content, total phenolic compounds, free amino acid, total soluble protein, and peroxidase activity were significantly affected by Beltanol and chloramphenicol (100 mg/l). By contrast, low chloramphenicol concentrations resulted in acceptable levels of biochemical components. Therefore, we recommend the use of low chloramphenicol and Beltanol concentrations instead of Switch and gentamycin as decontamination agents to prevent or treat microbial contamination in date palm tissue cultures.

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Effect of Ammonium Nitrate on Antioxidants Production of Date Palm (Phoenix dactylifera L.)in vitro

Cited by 11 publications

References 16 publications

Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium

Differential expression of anthocyanin biosynthesis genes in Daucus carota callus culture in response to ammonium and potassium nitrate ratio in the culture medium

Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee’

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

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