Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

Chudobová, Dagmar; Dostálová, Simona; Blažková, Iva; Michálek, Petr; Ruttkay-Nedecký, Branislav; Sklenar, Matej; Nejdl, Lukáš; Kudr, Jiří; Gumulec, Jaromír; Tmejová, Kateřina; Konečná, Marie; Vaculovičová, Markéta; Hynek, David; Masařík, Michal; Kynický, Jindřích; Kizek, René; Adam, Vojtěch

doi:10.3390/ijerph110303233

Cited by 56 publications

(44 citation statements)

References 64 publications

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“…The strains of the bacterium were stored as a spore suspension in 20% ( v/v ) glycerol at −20°C according to the conditions mentioned in Chudobova et al [36, 37]. Prior to use in this study, the strains were thawed and the glycerol was removed by washing with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8–11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.

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Section: Methodsmentioning

confidence: 99%

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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“…In terms of biological properties, the effect of UV radiation (laboratory and stratospheric) on the resistance of bacterial strains to environmental externalities, such as conventional antibiotic drugs or their alternative as selenium nanoparticles [24,25], was further monitored. It was shown that the exposure of UV significantly weakened the resistance of bacteria, and the application of antibiotic drugs or nanoparticles became more effective.…”

Section: Resistancementioning

confidence: 99%

Effects of Stratospheric Conditions on the Viability, Metabolism and Proteome of Prokaryotic Cells

et al. 2015

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Abstract:The application of ultraviolet (UV) radiation to inhibit bacterial growth is based on the principle that the exposure of DNA to UV radiation results in the formation of cytotoxic lesions, leading to inactivation of microorganisms. Herein, we present the impacts of UV radiation on bacterial cultures' properties from the biological, biochemical and molecular biological perspective. For experiments, commercial bacterial cultures (Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) and isolates from patients with bacterial infections (Proteus mirabilis and Pseudomonas aeruginosa) were employed. The above-mentioned strains were exposed to UV using a laboratory source and to stratospheric UV using a 3D printed probe carried by a stratospheric balloon. The length of flight was approximately two hours, and the probe was enriched by sensors for the external environment (temperature, pressure and relative humidity). After the landing, bacterial cultures were OPEN ACCESSAtmosphere 2015, 6 1291 cultivated immediately. Experimental results showed a significant effect of UV radiation (both laboratory UV and UV from the stratosphere) on the growth, reproduction, behavior and structure of bacterial cultures. In all parts of the experiment, UV from the stratosphere showed stronger effects when compared to the effects of laboratory UV. The growth of bacteria was inhibited by more than 50% in all cases; moreover, in the case of P. aeruginosa, the growth was even totally inhibited. Due to the effect of UV radiation, an increased susceptibility of bacterial strains to environmental influences was also observed. By using commercial tests for biochemical markers of Gram-positive and Gram-negative strains, significant disparities in exposed and non-exposed strains were found. Protein patterns obtained using MALDI-TOF mass spectrometry revealed that UV exposure is able to affect the proteins' expression, leading to their downregulation, observed as the disappearance of their peaks from the mass spectrum.

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“…Agricultural practices have contributed as a major source of heavy metals and antibiotics in contamination of water and soils (Zhu et al 2013). Moreover, metal ions accumulated to critical concentration levels in the environment promote the development of bacterial resistance to other xenobiotics, including antibiotics, which may impact public health (Seiler and Berendonk 2012;Chudobova et al 2014). Therefore, the co-selections of c Fig.…”

Section: Effects Of Zn Concentration and Binary Exposure On Bacterialmentioning

confidence: 98%

“…More and more bacterial strains have been found to be resistant to both heavy metals and antibiotics in different niches, including petroleum hydrocarbon contaminated soils (Máthé et al 2012), agricultural soils , surface water (Koc et al 2013), mangrove (Zhang et al 2012a;Pan et al 2010) and even Antarctic shallow sediments (Giudice et al 2013). Heavy metals have been established as one of the antibiotic resistance determinants (Martinez 2009;Chudobova et al 2014). Moreover, bacteria may pose threat to human and ecological health (Martinez 2008;Hazen et al 2010) once antibiotic resistance genes are acquired by environmental isolates including pathogens through gene transfer , which deserves more attention in both research and management.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative responses of Pseudomonas fluorescens YZ2 to simultaneous exposure of Zn and Cefradine

Chen

et al. 2015

Ecotoxicology

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Binary pollution of both heavy metals and antibiotics has received increasing attentions for their joint effects of eco-toxicity and health hazards. To reveal the effects of mixtures of different pollutants on bacterial antioxidant response system, Pseudomonas fluorescens ZY2, a new strain isolated from swine wastewater, was chosen to determinate growth (bacterial density OD600), reactive oxygen species (ROS) concentration, protein concentration and superoxide dismutase (SOD) activity under exposure treatments of Zn, Cefradine or Zn + Cefradine. Bacterial densities of all the treatment groups increased significantly over the incubation time, but those containing pollutant addition were slightly lower than the control at different times of incubation. Both ROS concentration and SOD activity increased first and then decreased (p < 0.01) over time, which was opposite to the protein concentrations (p < 0.01), showing a much significant increase by Cefradine alone. With Zn concentration increasing from 40 to 160 mg/L, the intracellular SOD activity increased as a response to the improvement of ROS (p < 0.05), while the balance between ROS and SOD was broken down due to the disproportionate change of total SOD activity and ROS concentration, the bacterial densities therefore decreased for the weak resistance. With the combined treatment of Zn (200 mg/L) and Cefradine (1 mg/L), though the toxicity of Zn caused a much significant increase of ROS, the bacterial resistance was further improved showing a more significant increase of total SOD activity and the bacterial densities therefore increased bacterial growth. Zn concentration also affected the protein synthesis. Either single or binary stress induced the bacterial resistance by regulating SOD activity to eliminate ROS. All results of the bacterial oxidant stress, SOD response and protein synthesis in the combined treatment groups were more complicated than those in single treatment groups, which depended on the properties of the single treatment as well as the interaction between the two treatments upon bacterial activity. For P. fluorescens ZY2, the mediation of SOD activity to eliminate ROS in response to the combined exposure to Zn and Cefradine was first revealed as one of the co-resistance mechanisms, which is informative to further understanding the risk of antibiotics resistant bacteria to human and environmental health more accurately.

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Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

Cited by 56 publications

References 64 publications

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Effects of Stratospheric Conditions on the Viability, Metabolism and Proteome of Prokaryotic Cells

Antioxidative responses of Pseudomonas fluorescens YZ2 to simultaneous exposure of Zn and Cefradine

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