Background: Foodborne diseases are challenge to global health. These diseases are caused by consuming contaminated food and can range in severity from mild discomfort to life-threatening illness. Therefore, food safety healthy instructions can help in preventing the spread of food borne diseases especially in primary school stage. Aim: Evaluate the effect of self-learning regarding food borne diseases on knowledge and practices of primary school teachers. Subjects and Method: Study design: a quasi-experimental research design. Setting: This study was carried out in ten primary schools which were selected randomly from (forty -seven) schools which are the total number of primary schools at Tanta city. Subjects: The total sample was 150 primary school teachers were chosen by systematic random sample technique. Tools of the study: Two tools were used to collect the needed data, Tool I: A structured questionnaire sheet to assess socio demographic characteristics of the teachers and teachers' knowledge regarding food borne diseases. Tool ІІ: Teachers' reported practices regarding prevention of food borne diseases. Results: The majority (89.3%) of the studied school teachers had poor knowledge score before self-learning intervention while after self-learning intervention there was an amazing improvement in their knowledge as 99.3% of them had good knowledge score. In addition, the majority (93.3%) of the studied school teachers reported unsatisfactory practices before self-learning, while 88% of them reported satisfactory practices after self-learning intervention. Conclusion: Self-learning intervention was effective in improving in teacher's knowledge and practices regarding food borne diseases. Recommendations: Encouraging continuous training and self-learning of primary school teachers to enhance their knowledge and practices related to food safety and prevention of food borne diseases.