Effect of an essential oil‐containing antiseptic mouthrinse on induction of platelet aggregation by oral bacteria in vitro

Whitaker, Eugene J.; Pham, Khoa; Feik, Diane; Rams, Thomas E.; Barnett, Michael L.; Pan, Pauline C.

doi:10.1034/j.1600-051x.2000.027005370.x

Cited by 9 publications

(12 citation statements)

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“…Listerine had a faster and stronger effect than did Xipayi. The essential-oil mouth rinse has the ability to affect cell surface-associated activities, and interfere with their main functions [17]. The mechanism for the anti-plaque activity is the disruption of the cell wall and the inhibition of bacterial enzyme systems [30].…”

Section: Discussionmentioning

confidence: 99%

“…They were then centrifuged, the supernatant was removed, and the cells were washed three times by resuspending them in sterile PBS, centrifuging, and replacing the supernatant with sterile PBS [17]. The same amount of the solutions described above (30 μL) were added to the PRP (250 μL).…”

Section: Effect Of the Two Antimicrobial Drugs On The Bacteriamentioning

confidence: 99%

“…This process was repeated three times. The bacteria were then dispersed in sterile PBS to achieve an optical density (OD = 2.0 at 600 nm) corresponding to 2.0×10 8 cells/mL, as determined by counting in a hemocytometer [17].…”

Section: Bacterial Cell Treatmentmentioning

confidence: 99%

“…Some studies have found that oil-containing antiseptics may have additional effects on bacteria exposed to sublethal levels [16]. Whitaker et al showed that essentialoil mouth rinse can interfere with S. sanguinis and P. gingivalis cell surface-associated activities that can induce platelet aggregation in vitro [17]. Fine et al [18] found that rinsing with essential-oil mouth rinse has an impact on the subgingival plaque flora.…”

Section: Introductionmentioning

confidence: 99%

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Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

Chen

Zheng

et al. 2016

J Infect Dev Ctries

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Introduction: The purpose of this study was to observe platelet aggregation promoted by biofilms of Streptococcus sanguinis and Porphyromonas gingivalis and to evaluate the effect of two different mouth rinses on this process. Methodology: In the first experiment, the same amount of S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solution was added to an equivalent amount of platelet-rich plasma (PRP). Aggregation was measured using a recording platelet aggregometer. In the second experiment, S. sanguinis, P. gingivalis, S sanguinis + P. gingivalis mixed solutions were pretreated with either Listerine antiseptic mouth rinse or Xipayi mouth rinse for 3 minutes, 6 minutes, and 10 minutes, respectively. The same amount of solution was added to the PRP, and the inhibition of aggregation was measured. Results: In the first experiment, S. sanguinis and P. gingivalis were able to induce platelet aggregation. The aggregation rate of S. sanguinis + P. gingivalis was significantly lower than that of either S. sanguinis or P. gingivalis. In the second experiment, when S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solutions were pretreated with Listerine antiseptic mouth rinse for 3 minutes and Xipayi mouth rinse for 10 minutes, there was no significant platelet aggregation. Conclusions: Platelets could adhere to S. sanguinis or P. gingivalis, but when S. sanguinis was mixed with P. gingivalis, the aggregation rate was reduced significantly. Treatment with Listerine antiseptic mouth rinse or Xipayi mouth rinse inhibited the ability of the bacteria to induce platelet aggregation.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of the Two Antimicrobial Drugs On The Bacteriamentioning

confidence: 99%

Section: Bacterial Cell Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

Chen

Zheng

et al. 2016

J Infect Dev Ctries

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“…Among the existing chemical plaque control methods, mouth washing is currently mainly used, and its use tends to increase because it is easy to use and because it is easy to carry a mouthwash bottle [5]. The chemical plaque control agents include Chlorhexidine (CHX) [6], listerine ® [7], fluoride [8], and slightly acidic hypochlorous acid (HOCl) obtained through the electrolysis of water containing sodium chloride (NaCl) [9]. In particular, CHX is a bisguanide agent absorbed by hydroxyapatite, the tooth surface, and salivary mucin, and is slowly released, thereby changing into the active state.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the preventive effects of slightly acidic HOCl mouthwash and CHX mouthwash for oral diseases

Kim¹,

Nam²

2018

biomedicalresearch

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The purpose of this study was to compare the preventive effects of the slightly acidic hypochlorous acid (HOCl) mouthwash and chlorhexidine (CHX) mouthwash as a chemical plaque control agent for oral diseases. This study was conducted for 15 patients who had no systemic disease among the patients who visited M Dental Clinic in Busan, South Korea, excluding the patients with incomplete data. The patients were divided into three groups: five patients in the 0.005% CHX (Bukwang Pharm Co., Ltd.) gargling group, five in the slightly acidic HOCl gargling group, and five in the saline gargling group. The patients were instructed to gargle with 15 ml of each mouthwash for 1 minute, and to then spit saliva for 1 min to remove the remaining gargling solution. Then the halitosis, O'Leary index, dental caries activity, bacterial species, and motility were measured. As a result, these decreased in the CHX gargling group compared to the saline gargling group, but the difference was not statistically significant (P>0.005), while there were significant decreases in the HOCl gargling group. The O'Leary index, Snyder test, bacterial motility, and Filamentous more distinctly and effectively decreased in the HOCl gargling group than in the CHX gargling group, showing statistically significant differences (p<0.05). Based on this study, CHX, which is currently widely used in dentistry, can be said to be inconvenient to use due to its strong fragrance and taste, and there have been many reports on the side effects on the oral cavity of its long-term use. Therefore, if HOCl, which is harmless to the human body and has an extensive sterilizing power, is used instead of CHX, safe and effective oral care will be achieved.

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Identification of oral cavity biofilm forming bacteria and determination of their growth inhibition by Acacia arabica , Tamarix aphylla L. and Melia azedarach L. medicinal plants

Khalid

Hassani

Bilal

et al. 2017

Archives of Oral Biology

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Effect of an essential oil‐containing antiseptic mouthrinse on induction of platelet aggregation by oral bacteria in vitro

Abstract: This study provides additional evidence that the essential oil mouthrinse can interfere with bacterial cell surface-associated activities which may have clinical relevance.

Cited by 9 publications

References 10 publications

Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

Comparison of the preventive effects of slightly acidic HOCl mouthwash and CHX mouthwash for oral diseases

Identification of oral cavity biofilm forming bacteria and determination of their growth inhibition by Acacia arabica , Tamarix aphylla L. and Melia azedarach L. medicinal plants

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