Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells

Abstract

Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and Portland cement (PC) on the viability, adhesion, migration, and differentiation of human dental pulp stem cells (hDPSCs) were examined. Based on a Cell Counting Kit-8 assay, hDPSCs exposed to Exp extracts showed limited viability at 24 and 48 h, but displayed comparable viability to the control at 72 h. hDPSC treatment with Exp extracts enhanced cellular adhesion and migration according to in vitro scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition in vitro compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping.

Retracted: The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway

“…Another crucial finding was in relation to the migration rate and cell adherence enhancement observed in the miR-143-5p inhibitors group. This finding was in concert with that of a previously conducted study, which demonstrated that a new direct pulp-capping material containing an antibacterial resin monomer as well as Portland cement could potentially enhance the adhesion and migration of DPSCs [Yu et al, 2016]. A demineralized dentin matrix has displayed its ability in enhancing DPSC adhesion and migration [Liu et al, 2016].…”

Physicochemical properties and cytotoxicity of an experimental resin‐based pulp capping material containing the quaternary ammonium salt and Portland cement

“…This study aimed at developing a light-curable material for pulp capping with potential antibacterial properties and potential ability to induce mineralized tissue formation. In previous studies, this material retained contact inhibition antibacterial activity for at least 6 months (Yang et al 2014) and had excellent biocompatibility (Yu et al 2016). In the present study, the basic properties of the material were investigated and compared with two commercial pulp capping materials.…”

“…Notably, a high DC can improve biocompatibility, which has been demonstrated in a previous study (Yu et al . ), because less unreacted monomers are able to leach into the surrounding tissues and cause an inflammatory reaction (Gandolfi et al . , Mehdawi et al .…”

“…The experimental material contained PC and resin matrix at a mass ratio of 2 : 1 (Yang et al 2014, Yu et al 2016. The experimental resin matrix contained a 4 : 3 : 1 mass ratio of HEMA-Bis-GMA-TEGDMA mixture (94 wt%), MAE-DB monomer (5% wt%), photoinitiator CQ (0.5% wt%) and coinitiator EDMAB (0.5% wt%).…”

“…) and promoted the adhesion, migration and osteogenic differentiation of human dental pulp stem cells (hDPSCs) in vitro , indicating excellent biocompatibility (Yu et al . ).…”